IRESite record type: plasmid_with_promoter_and_putative_IRES_translationally_characterized
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: our_best_guess
The mRNA/+RNA description:
Putative spliced in vivo SV40 promoter-derived transcript produced from bicistronic plasmid pstemRL which
comprises renilla and firefly luciferases as the first and the second cistron, respectively and the whole
mouse VEGF 5' UTR (nt from -1014 to nt +3 of the original sequence) mRNA with a hairpin in front of the first
cistron.
The sequence ends at its 3'-end right after the poly(A) signal from SV40 mRNA and thus the 3'-UTR might be
slightly wrong.
The mRNA/+RNA sequence represented in the +DNA notation:
Warning: mRNA sequence when devoid of trailing 'A's is still not a substring of the plasmid sequence. Is it because an intron is spliced out? Stay calm then. :-)
Credibility of mRNA sequence: end-to-end_sequence_reverse_engineered_and_should_match_experiment
The name of the promoter used to express this mRNA: SV40
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing whole mouse VEGF 5' UTR (nt from -1014 to nt +3 of the original sequence) inserted between
renilla and firefly luciferase reporter genes.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid: VEGF