Description of the plasmid (facultative for promoter-less plasmid records): Part (from nt -803 to nt -461 of the original sequence)of 5' UTR from human ELG1 mRNA has been cloned between
the beta galactosidase and chloramphenicol acetyl transferase reporter genes.
This promoter-less variant of pBiCELG1 (IRESite ID: 487) was prepared by removing the CMV promoter using NruI
and HindIII restriction sites.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The description of the protein encoded in this ORF: chloramphenicol acetyl transferase
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 4368-5027
Tested ELG1 5' UTR sequence contains 35 AUG repeats.
The plasmid was used as the negative control for experiments in article A search for structurally similar
cellular internal ribosome entry sites (Baird et al., 2007; PMID:17591613).