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The nucleic acid data:
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IRESite record type:
natural_transcript
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IRESs:
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IRES:
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IRES trans-acting factor (ITAFS):
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IRES trans-acting factor (ITAF):
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Type of the interaction between ITAF and the RNA subject to translation: direct_interaction_with_rna
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OPTIONAL: The interacting RNA base range (if any): 268-286,310-326
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ITAF protein characteristics:
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ITAF abbreviated name: PTB-1
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ITAF fullname: polypyrimidine tract-binding protein isoform 1
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ITAF description (long): polypyrimidine tract-binding protein isoform 1 binds dsRNA with (CCU)n motif where n is at least 3. By SELEX
approach it was found it binds to 5'-CAGCCUGGUGCCUCUCUUUCGG-3' (Singh et al. (1995) Science 268:1173-1176)
but also UCUU or UCUUC within pyrimidine-rich sequence (Perez et al. (1997) RNA3:1334-1347).
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3.1.2. Organisms or in vitro systems where this ITAF was functionally studied:
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Organism or in vitro system where ITAF was shown:
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Necessity of ITAF for translation in this particular organism or system: required_but_available_internally
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Method used to demonstrate ITAF effect: in_vivo
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Organism or in vitro system where ITAF was shown:
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Necessity of ITAF for translation in this particular organism or system: required_and_must_be_supplemented
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Method used to demonstrate ITAF effect: in_vitro
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In vitro system used to demonstrate ITAF effect: rabbit reticulocytes lysate
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Remarks:
Data from Figures 3 (in vivo) and 4E,F (in vitro). The binding region and unchanged structure due to the
binding was shown in Figure 6D(i) and Supplementary material C.
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Citations:
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IRES trans-acting factor (ITAF):
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Type of the interaction between ITAF and the RNA subject to translation: direct_interaction_with_rna
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OPTIONAL: The interacting RNA base range (if any): 268-286,310-326
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ITAF protein characteristics:
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ITAF abbreviated name: PTB-2
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ITAF fullname: polypyrimidine tract-binding protein isoform 2
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ITAF description (long): polypyrimidine tract-binding protein isoform 2 binds (CCU)n motif
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3.2.2. Organisms or in vitro systems where this ITAF was functionally studied:
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Organism or in vitro system where ITAF was shown:
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Necessity of ITAF for translation in this particular organism or system: required_and_must_be_supplemented
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Method used to demonstrate ITAF effect: in_vitro
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In vitro system used to demonstrate ITAF effect: rabbit reticulocytes lysate
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Remarks:
Data from Figures 4E,F (in vitro).
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Citations:
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IRES trans-acting factor (ITAF):
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Type of the interaction between ITAF and the RNA subject to translation: no_direct_interaction_with_rna
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ITAF protein characteristics:
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ITAF abbreviated name: PTB-4
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ITAF fullname: polypyrimidine tract-binding protein isoform 4
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ITAF description (long): polypyrimidine tract-binding protein isoform 4 binds (CCU)n motif
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3.3.2. Organisms or in vitro systems where this ITAF was functionally studied:
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Organism or in vitro system where ITAF was shown:
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Necessity of ITAF for translation in this particular organism or system: no_effect_on_translation
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Method used to demonstrate ITAF effect: in_vitro
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In vitro system used to demonstrate ITAF effect: rabbit reticulocytes lysate
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Remarks:
Data from Figures 4E,F (in vitro).
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Citations:
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IRES trans-acting factor (ITAF):
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Type of the interaction between ITAF and the RNA subject to translation: direct_interaction_with_rna
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OPTIONAL: The interacting RNA base range (if any): 268-286,310-326
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ITAF protein characteristics:
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ITAF abbreviated name: nPTB
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ITAF fullname: polypyrimidine tract-binding protein isoform n
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ITAF description (long): neuronal polypyrimidine tract-binding protein isoform binds (CCU)n motif
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3.4.2. Organisms or in vitro systems where this ITAF was functionally studied:
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Organism or in vitro system where ITAF was shown:
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Necessity of ITAF for translation in this particular organism or system: required_and_must_be_supplemented
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Method used to demonstrate ITAF effect: in_vitro
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In vitro system used to demonstrate ITAF effect: rabbit reticulocytes lysate
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Remarks:
Data from Figures 4E,F (in vitro).
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Citations:
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IRES trans-acting factor (ITAF):
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Type of the interaction between ITAF and the RNA subject to translation: no_direct_interaction_with_rna
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ITAF protein characteristics:
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ITAF abbreviated name: La
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ITAF fullname: La autoantigen
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ITAF description (long): La autoantigen (p52), 52 kDa RNA binding protein, predominantly localized to nucleus, unwinds the dsRNA in
ATP-dependent manner, forms a dimer
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3.5.2. Organisms or in vitro systems where this ITAF was functionally studied:
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Organism or in vitro system where ITAF was shown:
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Necessity of ITAF for translation in this particular organism or system: no_effect_on_translation
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Method used to demonstrate ITAF effect: in_vitro
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In vitro system used to demonstrate ITAF effect: rabbit reticulocytes lysate
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Remarks:
Data from Figures 4E,F (in vitro).
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Citations:
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IRES trans-acting factor (ITAF):
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Type of the interaction between ITAF and the RNA subject to translation: no_direct_interaction_with_rna
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ITAF protein characteristics:
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ITAF abbreviated name: p97/DAP5/NAT1
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ITAF fullname: p97/DAP5/NAT1
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ITAF description (long): p97/DAP5/NAT1 cleavage product p86 oe eIF4G, 86 kDa, RNA binding protein
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3.6.2. Organisms or in vitro systems where this ITAF was functionally studied:
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Organism or in vitro system where ITAF was shown:
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Necessity of ITAF for translation in this particular organism or system: no_effect_on_translation
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Method used to demonstrate ITAF effect: in_vitro
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In vitro system used to demonstrate ITAF effect: rabbit reticulocytes lysate
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Remarks:
Data from Figures 4E,F (in vitro).
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Citations:
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IRES trans-acting factor (ITAF):
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Type of the interaction between ITAF and the RNA subject to translation: no_direct_interaction_with_rna
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ITAF protein characteristics:
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ITAF abbreviated name: ITAF45
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ITAF fullname: ITAF45 (murine proliferation-associated protein, Mpp1)
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ITAF description (long): proliferation-dependent protein that is not expressed in murine brain cells, its human homolog is called PAG24
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3.7.2. Organisms or in vitro systems where this ITAF was functionally studied:
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Organism or in vitro system where ITAF was shown:
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Necessity of ITAF for translation in this particular organism or system: no_effect_on_translation
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Method used to demonstrate ITAF effect: in_vitro
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In vitro system used to demonstrate ITAF effect: rabbit reticulocytes lysate
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Remarks:
Data from Figures 4E,F (in vitro).
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Citations:
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Regions with experimentally determined secondary structures:
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A region with the experimentally determined secondary structure:
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