IRESite_Id:487 characteristics of ELG1 IRES studied in pBiCELG1 plasmid

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `487`	Version: `3`
Originaly submitted by: Václav Vopálenský
Reviewed by: Václav Vopálenský	Last change: `2008-07-16 23:14:55`

IRESite record type:

plasmid_with_promoter_and_putative_IRES_translationally_characterized

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
hopefully_full-length_mRNA

The mRNA/+RNA description:

Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pBiCELG1 which comprises
beta galactosidase and chloramphenicol acetyl transferase as the first and the second cistron, respectively
and the part (from nt -803 to nt -461 of the original sequence)of 5' UTR from human ELG1 mRNA.
The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be
slightly wrong.

The mRNA/+RNA sequence represented in the +DNA notation:

agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc gagctcggat ccactagtaa cggccgccag 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

tgtgctggaa ttctgcagat atccatcaca ctggcggccg caattCCCGG GGATCGAAAG AGCCTGCTAA AGCAAAAAAG AAGTCACCAT GTCGTTTACT 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

TTGACCAACA AGAACGTGAT TTTCGTTGCC GGTCTGGGAG GCATTGGTCT GGACACCAGC AAGGAGCTGC TCAAGCGCGA TCCCGTCGTT TTACAACGTC 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

TTCCCAACAG TTGCGCAGCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTTCGAT CTTCCTGAGG 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

CCGATACTGT CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT ACGGTCAATC CGCCGTTTGT 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGGA CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

AAAACCGCCT CGCGGTGATG GTGCTGCGTT GGAGTTACGG CAGTTATCTG GAAGATCAGG ATATTTGGCG GATGAGCGGC ATTTTCCGTG ACGTCTCGTT 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT TCAGATGTGC 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

AGCGTGGTGG TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA AATCCCGAAT CTCTATCGTG CGGTGGTTGA 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

ACTGCACACC GCCGACGGCA CGCTGATTGA AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT GAACGGCAAG 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATATCCTG CTGATGAAGC 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

AGAACAACTT TAACGCCGTG CGCTGTTCGC ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG CTACGGCCTG TATGTGGTGG ATGAAGCCAA 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

TATTGAAACC CACGGCATGG TGCCAATGAA TCGTCTGACC GATGATCCGC GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT GCAGCGCGAT 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

CGTAATCACC CGAGTGTGAT CATCTGGTCG CTGGGGAATG AATCAGGCCA CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

CCCGCCCGGT GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC CGATGTACGC GCGCGTGGAT GAAGACCAGC CCTTCCCGGC 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

TGTGCCGAAA TGGTCCATCA AAAAATGGCT TTCGCTACCT GGAGAGACGC GCCCGCTGAT CCTTTGCGAA TACGCCCACG CGATGGGTAA CAGTCTTGGC 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

GGTTTCGCTA AATACTGGCA GGCGTTTCGT CAGTATCCCC GTTTACAGGG CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA TATGATGAAA 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

ACGGCAACCC GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC GATCGCCAGT TCTGTATGAA CGGTCTGGTC TTTGCCGACC GCACGCCGCA 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

TCCAGCGCTG ACGGAAGCAA AACACCAGCA GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT CCGTCATAGC 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

AACTGCCTGA ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA CGCGACCGCA TGGTCAGAAG CCGGGCACAT 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

CAGCGCCTGG CAGCAGTGGC GTCTGGCGGA AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA CCACCAGCGA AATGGATTTT 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC AGTCAGGCTT TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

GCGATCAGTT CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA TTGACCCTAA CGCCTGGGTC GAACGCTGGA AGGCGGCGGG 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

CCATTACCAG GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG AACGCTCACG CGTGGCAGCA TCAGGGGAAA 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

ACCTTATTTA TCAGCCGGAA AACCTACCGG ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG CATCCGGCGC 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

TTTTGACCGC TGGGATCTGC CATTGTCAGA CATGTATACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT GAATTATGGC 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

GCACATGGCT GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC GGCGGAATTA CAGCTGAGCG CCGGTCGCTA 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

CCATTACCAG TTGGTCTGGT GTCAAAAATA ATAATAACCG GGCAGGCCAT GTCTGCCCGT ATTTCGCGTA AGGAAATCCA TTATGTACTA TTTAAAAAAC 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

ACAAACTTTT GGATGTTCGG TTTATTCTTT TTCTTTTACT TTTTTATCAT GGGAGCCTAC TTCCCGTTTT TCCCGATTTG GCTACATGAC ATCAACCATA 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

TCAGCAAAAG TGATACGGGT ATTATTTTTG CCGCTATTTC TCTGTTCTCG CTATTATTCC AACCGCTGTT TGGTCTGCTT TCTGACAAAC TCGGCCTCGA 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

CTCTAGGCGG CCGCTCGACG CTAGCACTTT TGGTGGGCAT TTAAAAATGT GTGTGTATGT GTATATATGT ATGTGTATGT ATGTGTATAT ATGTATATGT 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

ATGTATGTAT CGCGTGTATG TGTGTATGTA TGCATGTGTA TGTATGTATA TGCATGTATG TGTATGTGTA TATATGTATG TGTGTGTATG TATATGTGTG 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

TGTATGTGTA TGTGTGTGTG TATGTGTGTG TGTATGTATG TATGTATGTA TATGTATTAT ACACATATAC ACATATTGGT TTTTTTAATC ATTTGAGAGT 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

TAGTTGAAGA TAAAAACCCA TCACCCCTAA ATGTATTCCA AAGAATAAGA ACATTGTTTT ATACATAGCA CACTTAACAA AATCAAGAAA TTTAACATTA 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

ATACAGTACT GTTACCTAAT CCGTAGTCGA TTTTCAAATT TTGTCAGTTG TTCCAATAAT GTCCTTTATA TATTCCCCGC CCAGCTCGAG atggagaaaa 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa TTTTTTTAAG GCAGTTATTG GTGCCCTTAA ACGCCTGGTT CTACGCCTGA 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

ATAAGTGATA ATAAGCGGAT GAATGGCAGA AATTCGCCGg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgTCCT AGAGGGCCCT 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

ATTctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

tgaccctgga aggtgccact cccactgtcc tttcctaata a
^5801      ^5811      ^5821      ^5831      ^5841

Credibility of mRNA sequence:

reverse_engineered_sequence_and_should_match_experiment_maybe_except_3UTR

The name of the plasmid:
pBiCELG1 The name of the promoter used to express this mRNA:
CMV

Description of the plasmid (facultative for promoter-less plasmid records):
Part (from nt -803 to nt -461 of the original sequence)of 5' UTR from human ELG1 mRNA has been cloned between the beta galactosidase and chloramphenicol acetyl transferase reporter genes.

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

no

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

no

A promoter reported in cDNA corresponding to IRES sequence:

no

Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:

Summary of experiments studying integrity of the in vivo transcripts in a particular host:

Integrity (uniformity) of mRNA tested using Northern-blot:
not_tested

Integrity (uniformity) of mRNA tested using RNase protection:
not_tested

Integrity (uniformity) of mRNA tested using 5'-RACE:
not_tested

Integrity (uniformity) of mRNA tested using primer extension :
not_tested

Integrity (uniformity) of mRNA tested using RT-PCR:
not_tested

Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR):
homogeneous_population_of_molecules_confirmed

Integrity (uniformity) of mRNA tested using RNAi:
not_tested

Integrity (uniformity) of mRNA tested using S1 nuclease mapping:
not_tested

Cryptic promoter presence was confirmed by expression from a promoter-less plasmid:
no_promoter_confirmed

Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter:
not_tested

Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks:
not_tested

The organism used:

Homo sapiens HEK293 (ATCC CRL-1573)

The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
ELG1

The origin of IRES in the plasmid:

nuclear

The donor organism of the IRES segment:

Homo sapiens HEK 293T/17 (ATCC CRL-11268)

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaATAAA CAAATAGGGG TTCCGCGCAC 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

GCCAGTATCT GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC TTGACCGACA ATTGCATGAA 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

GAATCTGCTT AGGGTTAGGC GTTTTGCGCT GCTTcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg tgctggaatt ctgcagatat ccatcacact ggcggccgca 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

attCCCGGGG ATCGAAAGAG CCTGCTAAAG CAAAAAAGAA GTCACCATGT CGTTTACTTT GACCAACAAG AACGTGATTT TCGTTGCCGG TCTGGGAGGC 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

ATTGGTCTGG ACACCAGCAA GGAGCTGCTC AAGCGCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCTGA ATGGCGAATG GCGCTTTGCC 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTTCGATCT TCCTGAGGCC GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

ACGATGCGCC CATCTACACC AACGTGACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT TGTTACTCGC TCACATTTAA 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

GGCCAGGACA GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA AACCGCCTCG CGGTGATGGT GCTGCGTTGG AGTTACGGCA 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

GTTATCTGGA AGATCAGGAT ATTTGGCGGA TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA ATCAGCGATT TCCATGTTGC 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

CACTCGCTTT AATGATGATT TCAGCCGCGC TGTACTGGAG GCTGAAGTTC AGATGTGCGG CGAGTTGCGT GACTACCTAC GGGTAACAGT TTCTTTATGG 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

CAGGGTGAAA CGCAGGTCGC CAGCGGCACC GCGCCTTTCG GCGGTGAAAT TATCGATGAG CGTGGTGGTT ATGCCGATCG CGTCACACTA CGTCTGAACG 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

TCGAAAACCC GAAACTGTGG AGCGCCGAAA TCCCGAATCT CTATCGTGCG GTGGTTGAAC TGCACACCGC CGACGGCACG CTGATTGAAG CAGAAGCCTG 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

CGATGTCGGT TTCCGCGAGG TGCGGATTGA AAATGGTCTG CTGCTGCTGA ACGGCAAGCC GTTGCTGATT CGAGGCGTTA ACCGTCACGA GCATCATCCT 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

CTGCATGGTC AGGTCATGGA TGAGCAGACG ATGGTGCAGG ATATCCTGCT GATGAAGCAG AACAACTTTA ACGCCGTGCG CTGTTCGCAT TATCCGAACC 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

ATCCGCTGTG GTACACGCTG TGCGACCGCT ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG CCAATGAATC GTCTGACCGA 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG CGAATGGTGC AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGAA 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT CGATCCTTCC CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

CCACCGATAT TATTTGCCCG ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG GTCCATCAAA AAATGGCTTT CGCTACCTGG 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

AGAGACGCGC CCGCTGATCC TTTGCGAATA CGCCCACGCG ATGGGTAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA GTATCCCCGT 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA TGATGAAAAC GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

CGCCGAACGA TCGCCAGTTC TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

GTTCCGTTTA TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC CTGCACTGGA TGGTGGCGCT GGATGGTAAG 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC GGGCAACTCT 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC GGGCACATCA GCGCCTGGCA GCAGTGGCGT CTGGCGGAAA ACCTCAGTGT 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

TAAGTGAAGC GACCCGCATT GACCCTAACG CCTGGGTCGA ACGCTGGAAG GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

TACACTTGCT GATGCGGTGC TGATTACGAA CGCTCACGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC AGCCGGAAAA CCTACCGGAT TGATGGTAGT 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

GGTCAAATGG CGATTACCGT TGATGTTGAA GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA GCAGAGCGGG 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTATACCCC 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

GTACGTCTTC CCGAGCGAAA ACGGTCTGCG CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA CATCAGCCGC 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

GCGACGACTC CTGGAGCCCG TCAGTATCGG CGGAATTACA GCTGAGCGCC GGTCGCTACC ATTACCAGTT GGTCTGGTGT CAAAAATAAT AATAACCGGG 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

CAGGCCATGT CTGCCCGTAT TTCGCGTAAG GAAATCCATT ATGTACTATT TAAAAAACAC AAACTTTTGG ATGTTCGGTT TATTCTTTTT CTTTTACTTT 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

TTTATCATGG GAGCCTACTT CCCGTTTTTC CCGATTTGGC TACATGACAT CAACCATATC AGCAAAAGTG ATACGGGTAT TATTTTTGCC GCTATTTCTC 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

TGTTCTCGCT ATTATTCCAA CCGCTGTTTG GTCTGCTTTC TGACAAACTC GGCCTCGACT CTAGGCGGCC GCTCGACGCT AGCACTTTTG GTGGGCATTT 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

AAAAATGTGT GTGTATGTGT ATATATGTAT GTGTATGTAT GTGTATATAT GTATATGTAT GTATGTATCG CGTGTATGTG TGTATGTATG CATGTGTATG 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

TATGTATATG CATGTATGTG TATGTGTATA TATGTATGTG TGTGTATGTA TATGTGTGTG TATGTGTATG TGTGTGTGTA TGTGTGTGTG TATGTATGTA 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

TGTATGTATA TGTATTATAC ACATATACAC ATATTGGTTT TTTTAATCAT TTGAGAGTTA GTTGAAGATA AAAACCCATC ACCCCTAAAT GTATTCCAAA 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

GAATAAGAAC ATTGTTTTAT ACATAGCACA CTTAACAAAA TCAAGAAATT TAACATTAAT ACAGTACTGT TACCTAATCC GTAGTCGATT TTCAAATTTT 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

GTCAGTTGTT CCAATAATGT CCTTTATATA TTCCCCGCCC AGCTCGAGat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

gggcgtaaTT TTTTTAAGGC AGTTATTGGT GCCCTTAAAC GCCTGGTTCT ACGCCTGAAT AAGTGATAAT AAGCGGATGA ATGGCAGAAA TTCGCCGgat 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgTCCTAG AGGGCCCTAT Tctatagtgt cacctaaatg ctagagctcg ctgatcagcc 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

gcatgctggg gatgcggTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG Cgcattaagc 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaCA 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcggA TCTGATCAAG AGACAGGATG 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

AGGATCGTTT CGCatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 
^8001      ^8011      ^8021      ^8031      ^8041      ^8051      ^8061      ^8071      ^8081      ^8091      

agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 
^8101      ^8111      ^8121      ^8131      ^8141      ^8151      ^8161      ^8171      ^8181      ^8191      

acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 
^8201      ^8211      ^8221      ^8231      ^8241      ^8251      ^8261      ^8271      ^8281      ^8291      

aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 
^8301      ^8311      ^8321      ^8331      ^8341      ^8351      ^8361      ^8371      ^8381      ^8391      

catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 
^8401      ^8411      ^8421      ^8431      ^8441      ^8451      ^8461      ^8471      ^8481      ^8491      

gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 
^8501      ^8511      ^8521      ^8531      ^8541      ^8551      ^8561      ^8571      ^8581      ^8591      

tcttctgagc gGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG 
^8601      ^8611      ^8621      ^8631      ^8641      ^8651      ^8661      ^8671      ^8681      ^8691      

GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAacttgttt attgcagctt 
^8701      ^8711      ^8721      ^8731      ^8741      ^8751      ^8761      ^8771      ^8781      ^8791      

ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
^8801      ^8811      ^8821      ^8831      ^8841      ^8851      ^8861      ^8871      ^8881      ^8891      

ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
^8901      ^8911      ^8921      ^8931      ^8941      ^8951      ^8961      ^8971      ^8981      ^8991      

aACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
^9001      ^9011      ^9021      ^9031      ^9041      ^9051      ^9061      ^9071      ^9081      ^9091      

GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 
^9101      ^9111      ^9121      ^9131      ^9141      ^9151      ^9161      ^9171      ^9181      ^9191      

GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 
^9201      ^9211      ^9221      ^9231      ^9241      ^9251      ^9261      ^9271      ^9281      ^9291      

GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
^9301      ^9311      ^9321      ^9331      ^9341      ^9351      ^9361      ^9371      ^9381      ^9391      

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
^9401      ^9411      ^9421      ^9431      ^9441      ^9451      ^9461      ^9471      ^9481      ^9491      

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
^9501      ^9511      ^9521      ^9531      ^9541      ^9551      ^9561      ^9571      ^9581      ^9591      

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
^9601      ^9611      ^9621      ^9631      ^9641      ^9651      ^9661      ^9671      ^9681      ^9691      

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
^9701      ^9711      ^9721      ^9731      ^9741      ^9751      ^9761      ^9771      ^9781      ^9791      

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
^9801      ^9811      ^9821      ^9831      ^9841      ^9851      ^9861      ^9871      ^9881      ^9891      

gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
^9901      ^9911      ^9921      ^9931      ^9941      ^9951      ^9961      ^9971      ^9981      ^9991      

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
^10001     ^10011     ^10021     ^10031     ^10041     ^10051     ^10061     ^10071     ^10081     ^10091     

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
^10101     ^10111     ^10121     ^10131     ^10141     ^10151     ^10161     ^10171     ^10181     ^10191     

actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
^10201     ^10211     ^10221     ^10231     ^10241     ^10251     ^10261     ^10271     ^10281     ^10291     

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
^10301     ^10311     ^10321     ^10331     ^10341     ^10351     ^10361     ^10371     ^10381     ^10391     

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
^10401     ^10411     ^10421     ^10431     ^10441     ^10451     ^10461     ^10471     ^10481     ^10491     

gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
^10501     ^10511     ^10521     ^10531     ^10541     ^10551     ^10561     ^10571     ^10581     ^10591     

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
^10601     ^10611     ^10621     ^10631     ^10641     ^10651     ^10661     ^10671     ^10681     ^10691     

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
^10701     ^10711     ^10721     ^10731     ^10741     ^10751     ^10761     ^10771     ^10781     ^10791     

aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
^10801     ^10811     ^10821     ^10831     ^10841     ^10851     ^10861     ^10871     ^10881     ^10891     

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc c
^10901     ^10911     ^10921     ^10931     ^10941     ^10951     ^10961     ^10971     ^10981

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

The total number of notable open-reading frames (ORFs):

2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Václav Vopálenský	Reviewed by: Václav Vopálenský

The abbreviated name of this ORF/gene:
LacZ

The description of the protein encoded in this ORF:
beta galactosidase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

533-3331

ORF

ORF position:

2

Version: `0`
Originaly submitted by: Václav Vopálenský	Reviewed by: Václav Vopálenský

The abbreviated name of this ORF/gene:
CAT

The description of the protein encoded in this ORF:
chloramphenicol acetyl transferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

4091-4750

Remarks:

Tested ELG1 5' UTR sequence contains 35 AUG repeats.

Citations:

Baird S. D., Lewis S. M., Turcotte M., Holcik M. (2007) A search for structurally similar cellular internal ribosome entry sites. Nucleic. Acids. Res. 35(14):4664-4677

IRESs:

IRES:

Version: `3`	Last change: `2008-10-27 17:51:20`
Originaly submitted by: Václav Vopálenský	Reviewed by: Václav Vopálenský
The IRES name: `ELG1`	The functional status of IRES: `functional`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

3626-4085

How IRES boundaries were determined:
guessed

5'-end of IRES relative to last base of the STOP codon of the upstream ORF: `295`	3'-end of IRES relative to last base of the STOP codon of the upstream ORF: `754`
5'-end of IRES relative to first base of the START codon of the downstream ORF: `-465`	3'-end of IRES relative to first base of the START codon of the downstream ORF: `-6`

The sequence of IRES region aligned to its secondary structure (if available):

ACTTTTGGTG GGCATTTAAA AATGTGTGTG TATGTGTATA TATGTATGTG TATGTATGTG TATATATGTA TATGTATGTA TGTATCGCGT GTATGTGTGT 
^3626      ^3636      ^3646      ^3656      ^3666      ^3676      ^3686      ^3696      ^3706      ^3716      

ATGTATGCAT GTGTATGTAT GTATATGCAT GTATGTGTAT GTGTATATAT GTATGTGTGT GTATGTATAT GTGTGTGTAT GTGTATGTGT GTGTGTATGT 
^3726      ^3736      ^3746      ^3756      ^3766      ^3776      ^3786      ^3796      ^3806      ^3816      

GTGTGTGTAT GTATGTATGT ATGTATATGT ATTATACACA TATACACATA TTGGTTTTTT TAATCATTTG AGAGTTAGTT GAAGATAAAA ACCCATCACC 
^3826      ^3836      ^3846      ^3856      ^3866      ^3876      ^3886      ^3896      ^3906      ^3916      

CCTAAATGTA TTCCAAAGAA TAAGAACATT GTTTTATACA TAGCACACTT AACAAAATCA AGAAATTTAA CATTAATACA GTACTGTTAC CTAATCCGTA 
^3926      ^3936      ^3946      ^3956      ^3966      ^3976      ^3986      ^3996      ^4006      ^4016      

GTCGATTTTC AAATTTTGTC AGTTGTTCCA ATAATGTCCT TTATATATTC CCCGCCCAGC
^4026      ^4036      ^4046      ^4056      ^4066      ^4076

Remarks:

ELG1 IRES represents part (from nt -803 to nt -461 of the original sequence) of 5' UTR from human ELG1 mRNA.

The translation experiments:

Translation results:

IRESite Translation Id: 539

Version: `1`	Last change: `2009-09-03 19:20:56`
Originaly submitted by: Václav Vopálenský	Reviewed by: Václav Vopálenský

The translation method used to study IRES function:
in vivo

The organism used for translation:

Homo sapiens HEK293 (ATCC CRL-1573)

The temperature (in degrees of Celsia):
37

The relative translation efficiency in % of this IRES:

7.300

Name of IRES used as the positive control:

XIAP

Name of the plasmid used as the positive control. `pbetaGAL/5'(-162)/CAT`	Name of the plasmid used as the negative control. `pBiCmod`
`IRESite Id` of the plasmid used as positive control. `486`	`IRESite Id` of the plasmid used as negative control. `636`
The relative translation efficiency in % of the positive control: `100.000`	The relative translation efficiency in % of the negative control: `0.300`
The size (length) of intercistronic region in the positive control: `504`	The size (length) of intercistronic region in the negative control: `294`

The effect of 5'-cap analogs on translation:
not tested

Rapamycin affects translation:
not tested

Type of RNA subject to translation:

endogenous_nuclear_RNA_Pol_II_transcript

Remarks:

Translational data are derived from Fig. 4A.

Citations:

Baird S. D., Lewis S. M., Turcotte M., Holcik M. (2007) A search for structurally similar cellular internal ribosome entry sites. Nucleic. Acids. Res. 35(14):4664-4677

IRES trans-acting factor (ITAFS):

IRES trans-acting factor (ITAF):

Version: `0`
Originaly submitted by: Václav Vopálenský	Reviewed by: Václav Vopálenský

Type of the interaction between ITAF and the RNA subject to translation:
direct_interaction_with_rna

ITAF protein characteristics:

Version: `2`	Last change: `2009-08-29 12:19:15`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

ITAF abbreviated name:
La

ITAF fullname:
La autoantigen

ITAF description (long):
La autoantigen (p52), 52 kDa RNA binding protein, predominantly localized to nucleus, unwinds the dsRNA in ATP-dependent manner, forms a dimer

3.1.2. Organisms or in vitro systems where this ITAF was functionally studied:

Organism or in vitro system where ITAF was shown:

Necessity of ITAF for translation in this particular organism or system:
required_but_available_internally

Method used to demonstrate ITAF effect:
in_vitro In vitro system used to demonstrate ITAF effect:
other

Remarks:

HEK 293T cell extracts were used to demonstrate in vitro binding to ELG1 RNA. Data from Figure 8A.

Citations:

Baird S. D., Lewis S. M., Turcotte M., Holcik M. (2007) A search for structurally similar cellular internal ribosome entry sites. Nucleic. Acids. Res. 35(14):4664-4677

IRES trans-acting factor (ITAF):

Version: `0`
Originaly submitted by: Václav Vopálenský	Reviewed by: Václav Vopálenský

Type of the interaction between ITAF and the RNA subject to translation:
direct_interaction_with_rna

ITAF protein characteristics:

Version: `1`	Last change: `2008-06-27 20:14:51`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

ITAF abbreviated name:
hnRNP_A1

ITAF fullname:
heterogeneous nuclear ribonucleoprotein A1

ITAF description (long):
heterogeneous nuclear ribonucleoprotein A1 (p37), 37 kDa

3.2.2. Organisms or in vitro systems where this ITAF was functionally studied:

Organism or in vitro system where ITAF was shown:

Necessity of ITAF for translation in this particular organism or system:
required_but_available_internally

Method used to demonstrate ITAF effect:
in_vitro In vitro system used to demonstrate ITAF effect:
other

Remarks:

HEK 293T cell extracts were used to demonstrate in vitro binding to ELG1 RNA. Data from Figure 8A.

Citations:

Baird S. D., Lewis S. M., Turcotte M., Holcik M. (2007) A search for structurally similar cellular internal ribosome entry sites. Nucleic. Acids. Res. 35(14):4664-4677

Last change to the database: 2019-03-18 09:32:49 GMT+1