IRESite record type: negative_control_plasmid_with_promoter_and_without_putative_IRES
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: hopefully_full-length_mRNA
The mRNA/+RNA description:
Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid hp-pbetaGAL/CAT which
comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron,
respectively with a hairpin structure in front of the first cistron.
The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be
slightly wrong.
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: end-to-end_sequence_reverse_engineered_and_should_match_experiment
The name of the promoter used to express this mRNA: CMV
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing beta galactosidase and chloramphenicol acetyltransferase reporter genes. A synthetic DNA
fragment was inserted into the bicistronic constructs to introduce a stable stem loop structure 72 nt
downstream from CMV transcription start site.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:
GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
A promoter reported in cDNA corresponding to IRES sequence: not tested
The total number of notable open-reading frames (ORFs): 2
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence: