IRESite record type: plasmid_with_promoter_and_putative_IRES_translationally_characterized
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: 3UTR_possibly_incomplete
The mRNA/+RNA description:
Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid
pbetaGAL/HIAP2(870-1222)/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the
first and the second cistron respectively and the part of human c-IAP1 5' UTR (nt from -353 to nt -1 of the
original sequence) mRNA cloned between them.
The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: reverse_engineered_sequence_and_should_match_experiment_except_3UTR
The name of the promoter used to express this mRNA: CMV
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing part of human c-IAP1 5' UTR (nt from -353 to nt -1 of the original sequence) inserted
between beta galactosidase and chloramphenicol acetyltransferase reporter genes.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid: c-IAP1
The relative translation efficiency in % of this IRES: 124.200
Name of IRES used as the positive control: cIAP_-1222/-1
Name of the plasmid used as the positive control. pbetaGAL/HIAP2(-1222/-1)/CAT
IRESite Id of the plasmid used as positive control. 524
The relative translation efficiency in % of the positive control: 100.000
The size (length) of intercistronic region in the positive control: 1565
The effect of 5'-cap analogs on translation: not tested
Rapamycin affects translation: not tested
Type of RNA subject to translation: endogenous_nuclear_RNA_Pol_II_transcript
The relative IRES activity was determined in p86-cotrasfected HEK293T cells because the caspase-mediated
cleavage fragment of p97/DAP5/NAT1, p86, selectively enhances translation of c-IAP1 IRES element both in vivo
and in vitro in a manner that is independent of the translation of the upstream cistron
(Warnakulasuriyarachchi et al., 2004).
Translational data are derived from Fig. 5A.