A promoter reported in cDNA corresponding to IRES sequence: no
The total number of notable open-reading frames (ORFs): 1
Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:
Summary of experiments studying integrity of the in vivo transcripts in a particular host:
Integrity (uniformity) of mRNA tested using Northern-blot: homogeneous_population_of_molecules_confirmed
Integrity (uniformity) of mRNA tested using RNase protection: not_tested
Integrity (uniformity) of mRNA tested using 5'-RACE: not_tested
Integrity (uniformity) of mRNA tested using primer extension : not_tested
Integrity (uniformity) of mRNA tested using RT-PCR: not_tested
Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR): not_tested
Integrity (uniformity) of mRNA tested using RNAi: not_tested
Integrity (uniformity) of mRNA tested using S1 nuclease mapping: not_tested
Cryptic promoter presence was confirmed by expression from a promoter-less plasmid: no_promoter_confirmed
Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter: not_tested
Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks: not_tested
The description of the protein encoded in this ORF: angiotensin II receptor, type 1
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: no
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 382-1461
OPTIONAL: The function of the encoded protein
GO:0005886; Cellular component: plasma membrane
GO:0016021; Cellular component: integral to membrane
GO:0005887; Cellular component: integral to plasma membrane
GO:0004872; Molecular function: receptor activity
GO:0004947; Molecular function: bradykinin receptor activity
GO:0001584; Molecular function: rhodopsin-like receptor activity
GO:0016494; Molecular function: C-X-C chemokine receptor activity
GO:0004945; Molecular function: angiotensin type II receptor activity
GO:0007589; Biological process: fluid secretion
GO:0008015; Biological process: circulation
GO:0007165; Biological process: signal transduction
GO:0007204; Biological process: elevation of cytosolic calcium ion concentration
GO:0007186; Biological process: G-protein coupled receptor protein signaling pathway
GO:0007200; Biological process: G-protein signaling, coupled to IP3 second messenger (phospholipase C
activating)
Remarks:
Integrity of the bicistronic transcripts transcribed in vivo was verified by Northern blot analysis using cDNA
probes against firefly luciferase. Cryptic promoter existence within the AT1R 5'-UTR was ruled out using a
promoter-less plasmid and subsequent measurement of luciferase activity in cells (1/30 of the positive control
containing SV40 promoter and enhancer).
The IRES name: AT1R_var1 Warning: please make ires_name same as the gene_name and optionally append to it coordinates. E.g. when gene/virus name is EMCV-R use EMCV-R_-222_to_-1 or EMCV-R_1-456, etc. but not Emcv-R-... or EMCV-222_to_-1. Please keep case of letters as well. This rewards when searching through the database.
The IRES absolute position (the range includes START and STOP codons or their equivalents): 26-381
Conclusion: putative_IRES
How IRES boundaries were determined: guessed
The sequence of IRES region aligned to its secondary structure (if available):