IRESite_Id:97 negative control plasmid pFGAL4-L270

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `97`	Version: `3`
Originaly submitted by: Tomáš Mašek	Submission date: `2005-12-15 00:00:00`
Reviewed by: Tomáš Mašek	Last change: `2008-07-17 17:21:45`

IRESite record type:

negative_control_plasmid_with_promoter_and_without_putative_IRES

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
both_UTRs_incomplete

The mRNA/+RNA description:

mRNA produced by tricistronic plasmid pFGAL4-L270 which comprises luciferase, L270 and Gal4 ORFs. L270 clone
was selected from pFGAL4::lambda library containing randomly cleaved phage lambda DNA for its low expression
of Gal4p in vivo.

The mRNA/+RNA sequence represented in the +DNA notation:

ATGGAAGACG CCAAAAACAT AAAGAAAGGC CCGGCGCCAT TCTATCCGCT GGAAGATGGA ACCGCTGGAG AGCAACTGCA TAAGGCTATG AAGAGATACG 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

CCCTGGTTCC TGGAACAATT GCTTTTACAG ATGCACATAT CGAGGTGGAC ATCACTTACG CTGAGTACTT CGAAATGTCC GTTCGGTTGG CAGAAGCTAT 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

GAAACGATAT GGGCTGAATA CAAATCACAG AATCGTCGTA TGCAGTGAAA ACTCTCTTCA ATTCTTTATG CCGGTGTTGG GCGCGTTATT TATCGGAGTT 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

GCAGTTGCGC CCGCGAACGA CATTTATAAT GAACGTGAAT TGCTCAACAG TATGGGCATT TCGCAGCCTA CCGTGGTGTT CGTTTCCAAA AAGGGGTTGC 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

AAAAAATTTT GAACGTGCAA AAAAAGCTCC CAATCATCCA AAAAATTATT ATCATGGATT CTAAAACGGA TTACCAGGGA TTTCAGTCGA TGTACACGTT 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

CGTCACATCT CATCTACCTC CCGGTTTTAA TGAATACGAT TTTGTGCCAG AGTCCTTCGA TAGGGACAAG ACAATTGCAC TGATCATGAA CTCCTCTGGA 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

TCTACTGGTC TGCCTAAAGG TGTCGCTCTG CCTCATAGAA CTGCCTGCGT GAGATTCTCG CATGCCAGAG ATCCTATTTT TGGCAATCAA ATCATTCCGG 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

ATACTGCGAT TTTAAGTGTT GTTCCATTCC ATCACGGTTT TGGAATGTTT ACTACACTCG GATATTTGAT ATGTGGATTT CGAGTCGTCT TAATGTATAG 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ATTTGAAGAA GAGCTGTTTC TGAGGAGCCT TCAGGATTAC AAGATTCAAA GTGCGCTGCT GGTGCCAACC CTATTCTCCT TCTTCGCCAA AAGCACTCTG 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

ATTGACAAAT ACGATTTATC TAATTTACAC GAAATTGCTT CTGGTGGCGC TCCCCTCTCT AAGGAAGTCG GGGAAGCGGT TGCCAAGAGG TTCCATCTGC 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

CAGGTATCAG GCAAGGATAT GGGCTCACTG AGACTACATC AGCTATTCTG ATTACACCCG AGGGGGATGA TAAACCGGGC GCGGTCGGTA AAGTTGTTCC 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

ATTTTTTGAA GCGAAGGTTG TGGATCTGGA TACCGGGAAA ACGCTGGGCG TTAATCAAAG AGGCGAACTG TGTGTGAGAG GTCCTATGAT TATGTCCGGT 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

TATGTAAACA ATCCGGAAGC GACCAACGCC TTGATTGACA AGGATGGATG GCTACATTCT GGAGACATAG CTTACTGGGA CGAAGACGAA CACTTCTTCA 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

TCGTTGACCG CCTGAAGTCT CTGATTAAGT ACAAAGGCTA TCAGGTGGCT CCCGCTGAAT TGGAATCCAT CTTGCTCCAA CACCCCAACA TCTTCGACGC 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

AGGTGTCGCA GGTCTTCCCG ACGATGACGC CGGTGAACTT CCCGCCGCCG TTGTTGTTTT GGAGCACGGA AAGACGATGA CGGAAAAAGA GATCGTGGAT 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

TACGTCGCCA GTCAAGTAAC AACCGCGAAA AAGTTGCGCG GAGGAGTTGT GTTTGTGGAC GAAGTACCGA AAGGTCTTAC CGGAAAACTC GACGCAAGAA 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

AAATCAGAGA GATCCTCATA AAGGCCAAGA AGGGCGGAAA GATCGCCGTG TAATTCTAGC CTAGGGCCCA CAAGCTTGAA TTGTTTTATT TACTATCACA 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

AGATTAGTAG ATGTCGTTGG CGAAAAGTCA TTAGTTTTAT TCGATGAACC AGAGGTTCAT CTGCATCCAC CTTTGCTCTC TGCTTTTTTA CGAACATTAA 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

GCGACTTACT CGATGCACGC AATGGTGTAG CAATAATTCA GATCTCTCAC CTACCAAACA ATGCCCCCCT GCAAAAAATA AATTAACATC GCTCTTGAGC 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

AAAAAGGGTC CGGGAATTCA AAAATGAAGC TACTGTCTTC TATCGAACAA GCATGCGATA TTTGCCGACT TAAAAAGCTC AAGTGCTCCA AAGAAAAACC 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

GAAGTGCGCC AAGTGTCTGA AGAACAACTG GGAGTGTCGC TACTCTCCCA AAACCAAAAG GTCTCCGCTG ACTAGGGCAC ATCTGACAGA AGTGGAATCA 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

AGGCTAGAAA GACTGGAACA GCTATTTCTA CTGATTTTTC CTCGAGAAGA CCTTGACATG ATTTTGAAAA TGGATTCTTT ACAGGATATA AAAGCATTGT 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

TAACAGGATT ATTTGTACAA GATAATGTGA ATAAAGATGC CGTCACAGAT AGATTGGCTT CAGTGGAGAC TGATATGCCT CTAACATTGA GACAGCATAG 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

AATAAGTGCG ACATCATCAT CGGAAGAGAG TAGTAACAAA GGTCAAAGAC AGTTGACTGT ATCGATTGAC TCGGCAGCTC ATCATGATAA CTCCACAATT 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

CCGTTGGATT TTATGCCCAG GGATGCTCTT CATGGATTTG ATTGGTCTGA AGAGGATGAC ATGTCGGATG GCTTGCCCTT CCTGAAAACG GACCCCAACA 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

ATAATGGGTT CTTTGGCGAC GGTTCTCTCT TATGTATTCT TCGATCTATT GGCTTTAAAC CGGAAAATTA CACGAACTCT AACGTTAACA GGCTCCCGAC 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

CATGATTACG GATAGATACA CGTTGGCTTC TAGATCCACA ACATCCCGTT TACTTCAAAG TTATCTCAAT AATTTTCACC CCTACTGCCC TATCGTGCAC 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

TCACCGACGC TAATGATGTT GTATAATAAC CAGATTGAAA TCGCGTCGAA GGATCAATGG CAAATCCTTT TTAACTGCAT ATTAGCCATT GGAGCCTGGT 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

GTATAGAGGG GGAATCTACT GATATAGATG TTTTTTACTA TCAAAATGCT AAATCTCATT TGACGAGCAA GGTCTTCGAG TCAGGTTCCA TAATTTTGGT 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

GACAGCCCTA CATCTTCTGT CGCGATATAC ACAGTGGAGG CAGAAAACAA ATACTAGCTA TAATTTTCAC AGCTTTTCCA TAAGAATGGC CATATCATTG 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

GGCTTGAATA GGGACCTCCC CTCGTCCTTC AGTGATAGCA GCATTCTGGA ACAAAGACGC CGAATTTGGT GGTCTGTCTA CTCTTGGGAG ATCCAATTGT 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

CCCTGCTTTA TGGTCGATCC ATCCAGCTTT CTCAGAATAC AATCTCCTTC CCTTCTTCTG TCGACGATGT GCAGCGTACC ACAACAGGTC CCACCATATA 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

TCATGGCATC ATTGAAACAG CAAGGCTCTT ACAAGTTTTC ACAAAAATCT ATGAACTAGA CAAAACAGTA ACTGCAGAAA AAAGTCCTAT ATGTGCAAAA 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

AAATGCTTGA TGATTTGTAA TGAGATTGAG GAGGTTTCGA GACAGGCACC AAAGTTTTTA CAAATGGATA TTTCCACCAC CGCTCTAACC AATTTGTTGA 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

AGGAACACCC TTGGCTATCC TTTACAAGAT TCGAACTGAA GTGGAAACAG TTGTCTCTTA TCATTTATGT ATTAAGAGAT TTTTTCACTA ATTTTACCCA 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

GAAAAAGTCA CAACTAGAAC AGGATCAAAA TGATCATCAA AGTTATGAAG TTAAACGATG CTCCATCATG TTAAGCGATG CAGCACAAAG AACTGTTATG 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

TCTGTAAGTA GCTATATGGA CAATCATAAT GTCACCCCAT ATTTTGCCTG GAATTGTTCT TATTACTTGT TCAATGCAGT CCTAGTACCC ATAAAGACTC 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

TACTCTCAAA CTCAAAATCG AATGCTGAGA ATAACGAGAC CGCACAATTA TTACAACAAA TTAACACTGT TCTGATGCTA TTAAAAAAAC TGGCCACTTT 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

TAAAATCCAG ACTTGTGAAA AATACATTCA AGTACTGGAA GAGGTATGTG CGCCGTTTCT GTTATCACAG TGTGCAATCC CATTACCGCA TATCAGTTAT 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

AACAATAGTA ATGGTAGCGC CATTAAAAAT ATTGTCGGTT CTGCAACTAT CGCCCAATAC CCTACTCTTC CGGAGGAAAA TGTCAACAAT ATCAGTGTTA 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

AATATGTTTC TCCTGGCTCA GTAGGGCCTT CACCTGTGCC ATTGAAATCA GGAGCAAGTT TCAGTGATCT AGTCAAGCTG TTATCTAACC GTCCACCCTC 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

TCGTAACTCT CCAGTGACAA TACCAAGAAG CACACCTTCG CATCGCTCAG TCACGCCTTT TCTAGGGCAA CAGCAACAGC TGCAATCATT AGTGCCACTG 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

ACCCCGTCTG CTTTGTTTGG TGGCGCCAAT TTTAATCAAA GTGGGAATAT TGCTGATAGC TCATTGTCCT TCACTTTCAC TAACAGTAGC AACGGTCCGA 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

ACCTCATAAC AACTCAAACA AATTCTCAAG CGCTTTCACA ACCAATTGCC TCCTCTAACG TTCATGATAA CTTCATGAAT AATGAAATCA CGGCTAGTAA 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

AATTGATGAT GGTAATAATT CAAAACCACT GTCACCTGGT TGGACGGACC AAACTGCGTA TAACGCGTTT GGAATCACTA CAGGGATGTT TAATACCACT 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

ACAATGGATG ATGTATATAA CTATCTATTC GATGATGAAG ATACCCCACC AAACCCAAAA AAAGAGTAA
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561

Credibility of mRNA sequence:

end-to-end_sequence_completely_same_as_in_the_experiment

The name of the plasmid:
pFGAL4-L270 The name of the promoter used to express this mRNA:
TPI

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

GAATTAATTC ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT ACGCTGAGTA CTTCGAAATG TCCGTTCGGT 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

TGGCAGAAGC TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT TGGGCGCGTT 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTGC CAGAGTCCTT CGATAGGGAC AAGACAATTG CACTGATCAT 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

GAACTCCTCT GGATCTACTG GTCTGCCTAA AGGTGTCGCT CTGCCTCATA GAACTGCCTG CGTGAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

CAAATCATTC CGGATACTGC GATTTTAAGT GTTGTTCCAT TCCATCACGG TTTTGGAATG TTTACTACAC TCGGATATTT GATATGTGGA TTTCGAGTCG 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTCTGAGGAG CCTTCAGGAT TACAAGATTC AAAGTGCGCT GCTGGTGCCA ACCCTATTCT CCTTCTTCGC 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG CTTCTGGTGG CGCTCCCCTC TCTAAGGAAG TCGGGGAAGC GGTTGCCAAG 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

AGGTTCCATC TGCCAGGTAT CAGGCAAGGA TATGGGCTCA CTGAGACTAC ATCAGCTATT CTGATTACAC CCGAGGGGGA TGATAAACCG GGCGCGGTCG 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT GGATACCGGG AAAACGCTGG GCGTTAATCA AAGAGGCGAA CTGTGTGTGA GAGGTCCTAT 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG ACAAGGATGG ATGGCTACAT TCTGGAGACA TAGCTTACTG GGACGAAGAC 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

GAACACTTCT TCATCGTTGA CCGCCTGAAG TCTCTGATTA AGTACAAAGG CTATCAGGTG GCTCCCGCTG AATTGGAATC CATCTTGCTC CAACACCCCA 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

ACATCTTCGA CGCAGGTGTC GCAGGTCTTC CCGACGATGA CGCCGGTGAA CTTCCCGCCG CCGTTGTTGT TTTGGAGCAC GGAAAGACGA TGACGGAAAA 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

AGAGATCGTG GATTACGTCG CCAGTCAAGT AACAACCGCG AAAAAGTTGC GCGGAGGAGT TGTGTTTGTG GACGAAGTAC CGAAAGGTCT TACCGGAAAA 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

CTCGACGCAA GAAAAATCAG AGAGATCCTC ATAAAGGCCA AGAAGGGCGG AAAGATCGCC GTGTAATTCT AGCCTAGGGC CCACAAGCTT GAATTGTTTT 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

ATTTACTATC ACAAGATTAG TAGATGTCGT TGGCGAAAAG TCATTAGTTT TATTCGATGA ACCAGAGGTT CATCTGCATC CACCTTTGCT CTCTGCTTTT 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

TTACGAACAT TAAGCGACTT ACTCGATGCA CGCAATGGTG TAGCAATAAT TCAGATCTCT CACCTACCAA ACAATGCCCC CCTGCAAAAA ATAAATTAAC 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

ATCGCTCTTG AGCAAAAAGG GTCCGGGAAT TCAAAAATGA AGCTACTGTC TTCTATCGAA CAAGCATGCG ATATTTGCCG ACTTAAAAAG CTCAAGTGCT 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

CCAAAGAAAA ACCGAAGTGC GCCAAGTGTC TGAAGAACAA CTGGGAGTGT CGCTACTCTC CCAAAACCAA AAGGTCTCCG CTGACTAGGG CACATCTGAC 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

AGAAGTGGAA TCAAGGCTAG AAAGACTGGA ACAGCTATTT CTACTGATTT TTCCTCGAGA AGACCTTGAC ATGATTTTGA AAATGGATTC TTTACAGGAT 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

ATAAAAGCAT TGTTAACAGG ATTATTTGTA CAAGATAATG TGAATAAAGA TGCCGTCACA GATAGATTGG CTTCAGTGGA GACTGATATG CCTCTAACAT 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

TGAGACAGCA TAGAATAAGT GCGACATCAT CATCGGAAGA GAGTAGTAAC AAAGGTCAAA GACAGTTGAC TGTATCGATT GACTCGGCAG CTCATCATGA 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

TAACTCCACA ATTCCGTTGG ATTTTATGCC CAGGGATGCT CTTCATGGAT TTGATTGGTC TGAAGAGGAT GACATGTCGG ATGGCTTGCC CTTCCTGAAA 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

ACGGACCCCA ACAATAATGG GTTCTTTGGC GACGGTTCTC TCTTATGTAT TCTTCGATCT ATTGGCTTTA AACCGGAAAA TTACACGAAC TCTAACGTTA 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

ACAGGCTCCC GACCATGATT ACGGATAGAT ACACGTTGGC TTCTAGATCC ACAACATCCC GTTTACTTCA AAGTTATCTC AATAATTTTC ACCCCTACTG 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

CCCTATCGTG CACTCACCGA CGCTAATGAT GTTGTATAAT AACCAGATTG AAATCGCGTC GAAGGATCAA TGGCAAATCC TTTTTAACTG CATATTAGCC 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

ATTGGAGCCT GGTGTATAGA GGGGGAATCT ACTGATATAG ATGTTTTTTA CTATCAAAAT GCTAAATCTC ATTTGACGAG CAAGGTCTTC GAGTCAGGTT 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

CCATAATTTT GGTGACAGCC CTACATCTTC TGTCGCGATA TACACAGTGG AGGCAGAAAA CAAATACTAG CTATAATTTT CACAGCTTTT CCATAAGAAT 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

GGCCATATCA TTGGGCTTGA ATAGGGACCT CCCCTCGTCC TTCAGTGATA GCAGCATTCT GGAACAAAGA CGCCGAATTT GGTGGTCTGT CTACTCTTGG 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

GAGATCCAAT TGTCCCTGCT TTATGGTCGA TCCATCCAGC TTTCTCAGAA TACAATCTCC TTCCCTTCTT CTGTCGACGA TGTGCAGCGT ACCACAACAG 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

GTCCCACCAT ATATCATGGC ATCATTGAAA CAGCAAGGCT CTTACAAGTT TTCACAAAAA TCTATGAACT AGACAAAACA GTAACTGCAG AAAAAAGTCC 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

TATATGTGCA AAAAAATGCT TGATGATTTG TAATGAGATT GAGGAGGTTT CGAGACAGGC ACCAAAGTTT TTACAAATGG ATATTTCCAC CACCGCTCTA 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

ACCAATTTGT TGAAGGAACA CCCTTGGCTA TCCTTTACAA GATTCGAACT GAAGTGGAAA CAGTTGTCTC TTATCATTTA TGTATTAAGA GATTTTTTCA 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

CTAATTTTAC CCAGAAAAAG TCACAACTAG AACAGGATCA AAATGATCAT CAAAGTTATG AAGTTAAACG ATGCTCCATC ATGTTAAGCG ATGCAGCACA 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

AAGAACTGTT ATGTCTGTAA GTAGCTATAT GGACAATCAT AATGTCACCC CATATTTTGC CTGGAATTGT TCTTATTACT TGTTCAATGC AGTCCTAGTA 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

CCCATAAAGA CTCTACTCTC AAACTCAAAA TCGAATGCTG AGAATAACGA GACCGCACAA TTATTACAAC AAATTAACAC TGTTCTGATG CTATTAAAAA 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

AACTGGCCAC TTTTAAAATC CAGACTTGTG AAAAATACAT TCAAGTACTG GAAGAGGTAT GTGCGCCGTT TCTGTTATCA CAGTGTGCAA TCCCATTACC 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

GCATATCAGT TATAACAATA GTAATGGTAG CGCCATTAAA AATATTGTCG GTTCTGCAAC TATCGCCCAA TACCCTACTC TTCCGGAGGA AAATGTCAAC 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

AATATCAGTG TTAAATATGT TTCTCCTGGC TCAGTAGGGC CTTCACCTGT GCCATTGAAA TCAGGAGCAA GTTTCAGTGA TCTAGTCAAG CTGTTATCTA 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

ACCGTCCACC CTCTCGTAAC TCTCCAGTGA CAATACCAAG AAGCACACCT TCGCATCGCT CAGTCACGCC TTTTCTAGGG CAACAGCAAC AGCTGCAATC 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

ATTAGTGCCA CTGACCCCGT CTGCTTTGTT TGGTGGCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGT AAGAGCTCAG CTAGCTAACT 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

GAATAAGGAA CAATGAACGT TTTTCCTTTC TCTTGTTCCT AGTATTAATG ACTGACCGAT ACATCCCTTT TTTTTTTTGT CTTTGTCTAG CTCCAATTCG 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

CCCTATAGTG AGTCGTATTA CAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGACGCGCC CTGTAGCGGC 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT AGGGTAATAA 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

CTGATATAAT TAAATTGAAG CTCTAATTTG TGAGTTTAGT ATACATGCAT TTACTTATAA TACAGTTTTT TAGTTTTGCT GGCCGCATCT TCTCAAATAT 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

GCTTCCCAGC CTGCTTTTCT GTAACGTTCA CCCTCTACCT TAGCATCCCT TCCCTTTGCA AATAGTCCTC TTCCAACAAT AATAATGTCA GATCCTGTAG 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

AGACCACATC ATCCACGGTT CTATACTGTT GACCCAATGC GTCTCCCTTG TCATCTAAAC CCACACCGGG TGTCATAATC AACCAATCGT AACCTTCATC 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

TCTTCCACCC ATGTCTCTTT GAGCAATAAA GCCGATAACA AAATCTTTGT CGCTCTTCGC AATGTCAACA GTACCCTTAG TATATTCTCC AGTAGATAGG 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

GAGCCCTTGC ATGACAATTC TGCTAACATC AAAAGGCCTC TAGGTTCCTT TGTTACTTCT TCTGCCGCCT GCTTCAAACC GCTAACAATA CCTGGCCCCA 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

CCACACCGTG TGCATTCGTA ATGTCTGCCC ATTCTGCTAT TCTGTATACA CCCGCAGAGT ACTGCAATTT GACTGTATTA CCAATGTCAG CAAATTTTCT 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

GTCTTCGAAG AGTAAAAAAT TGTACTTGGC GGATAATGCC TTTAGCGGCT TAACTGTGCC CTCCATCGAA AAATCAGTCA ATATATCCAC ATGTGTTTTT 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

AGTAAACAAA TTTTGGGACC TAATGCTTCA ACTAACTCCA GTAATTCCTT GGTGGTACGA ACATCCAATG AAGCACACAA GTTTGTTTGC TTTTCGTGCA 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

TGATATTAAA TAGCTTGGCA GCAACAGGAC TAGGATGAGT AGCAGCACGT TCCTTATATG TAGCTTTCGA CATGATTTAT CTTCGTTTCC TGCAGGTTTT 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

TGTTCTGTGC AGTTGGGTTA AGAATACTGG GCAATTTCAT GTTTCTTCAA CACTACATAT GCGTATATAT ACCAATCTAA GTCTGTGCTC CTTCCTTCGT 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

TCTTCCTTCT GTTCGGAGAT TACCGAATCA AAAAAATTTC AAGGAAACCG AAATCAAAAA AAAGAATAAA AAAAAAATGA TGAATTGAAA AGGTGGTATG 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TGCGGCCGCT CTAGAACTAG TGGATCAATT CCACGGACTA TAGACTATAC 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

TAGTATACTC CGTCTACTGT ACGATACACT TCCGCTCAGG TCCTTGTCCT TTAACGAGGC CTTACCACTC TTTTGTTACT CTATTGATCC AGCTCAGCAA 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

AGGCAGTGTG ATCTAAGATT CTATCTTCGC GATGTAGTAA AACTAGCTAG ACCGAGAAAG AGACTAGAAA TGCAAAAGGC ACTTCTACAA TGGCTGCCAT 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

CATTATTATC CGATGTGACG CTGCAGCTTC TCAATGATAT TCGAATACGC TTTGAGGAGA TACAGCCTAA TATCCGACAA ACTGTTTTAC AGATTTACGA 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

TCGTACTTGT TACCCATCAT TGAATTTTGA ACATCCGAAC CTGGGAGTTT TCCCTGAAAC AGATAGTATA TTTGAACCTG TATAATAATA TATAGTCTAG 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

CGCTTTACGG AAGACAATGT ATGTATTTCG GTTCCTGGAG AAACTATTGC ATCTATTGCA TAGGTAATCT TGCACGTCGC ATCCCCGGTT CATTTTCTGC 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

GTTTCCATCT TGCACTTCAA TAGCATATCT TTGTTAACGA AGCATCTGTG CTTCATTTTG TAGAACAAAA ATGCAACGCG AGAGCGCTAA TTTTTCAAAC 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAAAGCGC TATTTTACCA ACGAAGAATC TGTGCTTCAT TTTTGTAAAA CAAAAATGCA 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

ACGCGAGAGC GCTAATTTTT CAAACAAAGA ATCTGAGCTG CATTTTTACA GAACAGAAAT GCAACGCGAG AGCGCTATTT TACCAACAAA GAATCTATAC 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

TTCTTTTTTG TTCTACAAAA ATGCATCCCG AGAGCGCTAT TTTTCTAACA AAGCATCTTA GATTACTTTT TTTCTCCTTT GTGCGCTCTA TAATGCAGTC 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

TCTTGATAAC TTTTTGCACT GTAGGTCCGT TAAGGTTAGA AGAAGGCTAC TTTGGTGTCT ATTTTCTCTT CCATAAAAAA AGCCTGACTC CACTTCCCGC 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

GTTTACTGAT TACTAGCGAA GCTGCGGGTG CATTTTTTCA AGATAAAGGC ATCCCCGATT ATATTCTATA CCGATGTGGA TTGCGCATAC TTTGTGAACA 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

GAAAGTGATA GCGTTGATGA TTCTTCATTG GTCAGAAAAT TATGAACGGT TTCTTCTATT TTGTCTCTAT ATACTACGTA TAGGAAATGT TTACATTTTC 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

GTATTGTTTT CGATTCACTC TATGAATAGT TCTTACTACA ATTTTTTTGT CTAAAGAGTA ATACTAGAGA TAAACATAAA AAATGTAGAG GTCGAGTTTA 
^8001      ^8011      ^8021      ^8031      ^8041      ^8051      ^8061      ^8071      ^8081      ^8091      

GATGCAAGTT CAAGGAGCGA AAGGTGGATG GGTAGGTTAT ATAGGGATAT AGCACAGAGA TATATAGCAA AGAGATACTT TTGAGCAATG TTTGTGGAAG 
^8101      ^8111      ^8121      ^8131      ^8141      ^8151      ^8161      ^8171      ^8181      ^8191      

CGGTATTCGC AATATTTTAG TAGCTCGTTA CAGTCCGGTG CGTTTTTGGT TTTTTGAAAG TGCGTCTTCA GAGCGCTTTT GGTTTTCAAA AGCGCTCTGA 
^8201      ^8211      ^8221      ^8231      ^8241      ^8251      ^8261      ^8271      ^8281      ^8291      

AGTTCCTATA CTTTCTAGAG AATAGGAACT TCGGAATAGG AACTTCAAAG CGTTTCCGAA AACGAGCGCT TCCGAAAATG CAACGCGAGC TGCGCACATA 
^8301      ^8311      ^8321      ^8331      ^8341      ^8351      ^8361      ^8371      ^8381      ^8391      

CAGCTCACTG TTCACGTCGC ACCTATATCT GCGTGTTGCC TGTATATATA TATACATGAG AAGAACGGCA TAGTGCGTGT TTATGCTTAA ATGCGTACTT 
^8401      ^8411      ^8421      ^8431      ^8441      ^8451      ^8461      ^8471      ^8481      ^8491      

ATATGCGTCT ATTTATGTAG GATGAAAGGT AGTCTAGTAC CTCCTGTGAT ATTATCCCAT TCCATGCGGG GTATCGTATG CTTCCTTCAG CACTACCCTT 
^8501      ^8511      ^8521      ^8531      ^8541      ^8551      ^8561      ^8571      ^8581      ^8591      

TAGCTGTTCT ATATGCTGCC ACTCCTCAAT TGGATTAGTC TCATCCTTCA ATGCTATCAT TTCCTTTGAT ATTGGATCAT ATGCATAGTA CCGAGAAACT 
^8601      ^8611      ^8621      ^8631      ^8641      ^8651      ^8661      ^8671      ^8681      ^8691      

AGTGCGAAGT AGTGATCAGG TATTGCTGTT ATCTGATGAG TATACGTTGT CCTGGCCACG GCAGAAGCAC GCTTATCGCT CCAATTTCCC ACAACATTAG 
^8701      ^8711      ^8721      ^8731      ^8741      ^8751      ^8761      ^8771      ^8781      ^8791      

TCAACTCCGT TAGGCCCTTC ATTGAAAGAA ATGAGGTCAT CAAATGTCTT CCAATGTGAG ATTTTGGGCC ATTTTTTATA GCAAAGATTG AATAAGGCGC 
^8801      ^8811      ^8821      ^8831      ^8841      ^8851      ^8861      ^8871      ^8881      ^8891      

ATTTTTCTTC AAAGCTGCGG CCGCACTCTC ACTAGTACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC 
^8901      ^8911      ^8921      ^8931      ^8941      ^8951      ^8961      ^8971      ^8981      ^8991      

ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 
^9001      ^9011      ^9021      ^9031      ^9041      ^9051      ^9061      ^9071      ^9081      ^9091      

TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT 
^9101      ^9111      ^9121      ^9131      ^9141      ^9151      ^9161      ^9171      ^9181      ^9191      

TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG 
^9201      ^9211      ^9221      ^9231      ^9241      ^9251      ^9261      ^9271      ^9281      ^9291      

CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 
^9301      ^9311      ^9321      ^9331      ^9341      ^9351      ^9361      ^9371      ^9381      ^9391      

TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG 
^9401      ^9411      ^9421      ^9431      ^9441      ^9451      ^9461      ^9471      ^9481      ^9491      

AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG 
^9501      ^9511      ^9521      ^9531      ^9541      ^9551      ^9561      ^9571      ^9581      ^9591      

ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 
^9601      ^9611      ^9621      ^9631      ^9641      ^9651      ^9661      ^9671      ^9681      ^9691      

GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC 
^9701      ^9711      ^9721      ^9731      ^9741      ^9751      ^9761      ^9771      ^9781      ^9791      

ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG 
^9801      ^9811      ^9821      ^9831      ^9841      ^9851      ^9861      ^9871      ^9881      ^9891      

AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 
^9901      ^9911      ^9921      ^9931      ^9941      ^9951      ^9961      ^9971      ^9981      ^9991      

CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 
^10001     ^10011     ^10021     ^10031     ^10041     ^10051     ^10061     ^10071     ^10081     ^10091     

TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 
^10101     ^10111     ^10121     ^10131     ^10141     ^10151     ^10161     ^10171     ^10181     ^10191     

TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 
^10201     ^10211     ^10221     ^10231     ^10241     ^10251     ^10261     ^10271     ^10281     ^10291     

GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 
^10301     ^10311     ^10321     ^10331     ^10341     ^10351     ^10361     ^10371     ^10381     ^10391     

AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 
^10401     ^10411     ^10421     ^10431     ^10441     ^10451     ^10461     ^10471     ^10481     ^10491     

AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 
^10501     ^10511     ^10521     ^10531     ^10541     ^10551     ^10561     ^10571     ^10581     ^10591     

CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 
^10601     ^10611     ^10621     ^10631     ^10641     ^10651     ^10661     ^10671     ^10681     ^10691     

AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT 
^10701     ^10711     ^10721     ^10731     ^10741     ^10751     ^10761     ^10771     ^10781     ^10791     

TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC 
^10801     ^10811     ^10821     ^10831     ^10841     ^10851     ^10861     ^10871     ^10881     ^10891     

CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT 
^10901     ^10911     ^10921     ^10931     ^10941     ^10951     ^10961     ^10971     ^10981     ^10991     

TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT 
^11001     ^11011     ^11021     ^11031     ^11041     ^11051     ^11061     ^11071     ^11081     ^11091     

ACGCCAAGCT CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG GGCCGGCCGT CGAGCTTGAT GGCATCGTGG TGTCACGCTC GTCGTTTGGT 
^11101     ^11111     ^11121     ^11131     ^11141     ^11151     ^11161     ^11171     ^11181     ^11191     

ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
^11201     ^11211     ^11221     ^11231     ^11241     ^11251     ^11261     ^11271     ^11281     ^11291     

TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 
^11301     ^11311     ^11321     ^11331     ^11341     ^11351     ^11361     ^11371     ^11381     ^11391     

TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT 
^11401     ^11411     ^11421     ^11431     ^11441     ^11451     ^11461     ^11471     ^11481     ^11491     

TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
^11501     ^11511     ^11521     ^11531     ^11541     ^11551     ^11561     ^11571     ^11581     ^11591     

ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 
^11601     ^11611     ^11621     ^11631     ^11641     ^11651     ^11661     ^11671     ^11681     ^11691     

TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
^11701     ^11711     ^11721     ^11731     ^11741     ^11751     ^11761     ^11771     ^11781     ^11791     

CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA 
^11801     ^11811     ^11821     ^11831     ^11841     ^11851     ^11861     ^11871     ^11881     ^11891     

GGCCCTTTCG TCTTCAAGAA TTGGGGATCT ACGTATGGTC ATTTCTTCTT CAGATTCCCT CATGGAGAAA GTGCGGCAGA TGTATATGAC AGAGTCGCCA 
^11901     ^11911     ^11921     ^11931     ^11941     ^11951     ^11961     ^11971     ^11981     ^11991     

GTTTCCAAGA GACTTTATTC AGGCACTTCC ATGATAGGCA AGAGAGAAGA CCCAGAGATG TTGTTGTCCT AGTTACACAT GGTATTTATT CCAGAGTATT 
^12001     ^12011     ^12021     ^12031     ^12041     ^12051     ^12061     ^12071     ^12081     ^12091     

CCTGATGAAA TGGTTTAGAT GGACATACGA AGAGTTTGAA TCGTTTACCA ATGTTCCTAA CGGGAGCGTA ATGGTGATGG AACTGGACGA ATCCATCAAT 
^12101     ^12111     ^12121     ^12131     ^12141     ^12151     ^12161     ^12171     ^12181     ^12191     

AGATACGTCC TGAGGACCGT GCTACCCAAA TGGACTGATT GTGAGGGAGA CCTAACTACA TAGTGTTTAA AGATTACGGA TATTTAACTT ACTTAGAATA 
^12201     ^12211     ^12221     ^12231     ^12241     ^12251     ^12261     ^12271     ^12281     ^12291     

ATGCCATTTT TTTGAGTTAT AATAATCCTA CGTTAGTGTG AGCGGGATTT AAACTGTGAG GACCTTAATA CATTCAGACA CTTCTGCGGT ATCACCCTAC 
^12301     ^12311     ^12321     ^12331     ^12341     ^12351     ^12361     ^12371     ^12381     ^12391     

TTATTCCCTT CGAGATTATA TCTAGGAACC CATCAGGTTG GTGGAAGATT ACCCGTTCTA AGACTTTTCA GCTTCCTCTA TTGATGTTAC ACCTGGACAC 
^12401     ^12411     ^12421     ^12431     ^12441     ^12451     ^12461     ^12471     ^12481     ^12491     

CCCTTTTCTG GCATCCAGTT TTTAATCTTC AGTGGCATGT GAGATTCTCC GAAATTAATT AAAGCAATCA CACAATTCTC TCGGATACCA CCTCGGTTGA 
^12501     ^12511     ^12521     ^12531     ^12541     ^12551     ^12561     ^12571     ^12581     ^12591     

AACTGACAGG TGGTTTGTTA CGCATGCTAA TGCAAAGGAG CCTATATACC TTTGGCTCGG CTGCTGTAAC AGGGAATATA AAGGGCAGCA TAATTTAGGA 
^12601     ^12611     ^12621     ^12631     ^12641     ^12651     ^12661     ^12671     ^12681     ^12691     

GTTTAGTGAA CTTGCAACAT TTACTATTTT CCCTTCTTAC GTAAATATTT TTCTTTTTAA TTCTAAATCA ATCTTTTTCA ATTTTTTGTT TGTATTCTTT 
^12701     ^12711     ^12721     ^12731     ^12741     ^12751     ^12761     ^12771     ^12781     ^12791     

TCTTGCTTAA ATCTATAACT ACAAAAAACA CATACAG
^12801     ^12811     ^12821     ^12831

A promoter reported in cDNA corresponding to IRES sequence:
not tested The total number of notable open-reading frames (ORFs):
3

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Tomáš Mašek	Reviewed by: Tomáš Mašek

The abbreviated name of this ORF/gene:
Fluc

The description of the protein encoded in this ORF:
firefly luciferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

no

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1-1653

ORF

ORF position:

2

Version: `0`
Originaly submitted by: Tomáš Mašek	Reviewed by: Tomáš Mašek

The abbreviated name of this ORF/gene:
L270

The description of the protein encoded in this ORF:
arteficial short ORF derived from bacteriophage lambda DNA (25802-25746, GI:215104)

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

no

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1744-1800

ORF

ORF position:

3

Version: `0`
Originaly submitted by: Tomáš Mašek	Reviewed by: Tomáš Mašek

The abbreviated name of this ORF/gene:
GAL4

The description of the protein encoded in this ORF:
DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

no

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1924-4569

OPTIONAL: The function of the encoded protein

Molecular Function: transcriptional activator activity (TAS)
Biological Process: galactose metabolism (TAS) regulation of transcription, DNA-dependent (TAS)
Cellular Component: nucleus (TAS)

Remarks:

L270 sequence was inserted into intercistronic position of pFGAL4 bicistronic plasmid to create tricistronic
control vector that can be used in the pFGAL4h/PJ69A reporter system. The yeast strain PJ69A contains besides
the GAL4 and GAL80 deletions also reporter genes coding for the yeast Ade2 and His3 proteins and for the
bacterial beta-galactosidase - all of them under the control of the Gal4-inducible Gal1 promoter. Thus
pFGAL4/PJ69A represents a specialised and sensitive system, which allows an enhancement of the measured signal
by in vivo coupled transcription and enzymatic detection. This system can be used for both measuring the ratio
of the beta-gal/luc enzymatic activities and searching for IRES activity by screening the colony growth rates
on selection media lacking adenine and histidine and containing various concentrations of the competitive
inhibitor of the histidine biosynthetic pathway.

Citations:

Masek T., Vopalensky V., Horvath O., Vortelova L., Feketova Z., Pospisek M. (2007) Hepatitis C virus internal ribosome entry site initiates protein synthesis at the authentic initiation codon in yeast. J. Gen. Virol. 88(Pt 7):1992-2002

Last change to the database: 2019-03-18 09:32:49 GMT+1