The nucleic acid data:
IRESite Id: 117 Version: 4
Originaly submitted by: Václav Vopálenský
Reviewed by: Václav Vopálenský Last change: 2009-09-02 01:47:02
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  hopefully_full-length_mRNA
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  DAP5
The genetic origin of this natural mRNA/+RNA:
  nuclear
The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
111494227
Synonyms of the gene name:
Synonym: p97
Synonym: AAG1
Synonym: NAT1
Synonym: FLJ41344
The mRNA/+RNA description: 
Death-associated protein 5 (DAP5) (also named p97 and NAT1), a 97-kDa protein homologous to eukaryotic
translation initiation factor 4GI (eIF4GI)
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  guessed_as_the_sequence_was_never_published_by_authors_nor_described_in_sufficient_detail
The organism containing this mRNA with IRES segment in its genome:
Homo sapiens
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
  1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 1 Last change: 2009-03-22 23:31:23
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The abbreviated name of this ORF/gene:
DAP5
The description of the protein encoded in this ORF:
death-associated protein 5
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  418-3141
Remarks:
IRESite annotation is based on NM_001418.3 sequence. The record NM_001418.1 contained CDS between positions
307-3030 which matched the comments in the article by Henis-Korenblit et al. (2000) that the 5'-UTR was about
300 bp long and in Imataka et al. (1997) that the GUG-initiated CDS encodes 907 aa residues. A sequence of the
cloned cDNA was shown in Figure 2 in Levy-Strumpf et al. (1997) and aligns with this RefSeq sequence since its
position 127 (thus positions 1-7 shown in the Figure 2 do not have a counterpart). The sequence of p97/DAP5
published by Imataka is in AF104913 (GI:3941723).

The forward primer described in Henis-Korenblit et al. (2000) aligns well to both these RefSeq sequences while
the reverse primer mismatches in several positions. EST sequences confirm this NM_001418.3 sequence since
position 81 downstream while such ESTs begin at that position (GI:83041139).

Although RefSeq annotates this sequence as eIF4G2 we removed this gene name from this record because the DNA
sequence differs from both eIF4GI and eIF4GII. Probably because Shaughnessy et al. (1997) called this 907 aa
residues spanning CDS eIF4G2.
Citations:
Henis-Korenblit S., Strumpf N. L., Goldstaub D., Kimchi A. (2000) A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation. Mol. Cell. Biol. 20(2):496-506
Imataka H., Olsen H. S., Sonenberg N. (1997) A new translational regulator with homology to eukaryotic translation initiation factor 4G. EMBO J. 16(4):817-825
Levy-Strumpf N., Deiss L. P., Berissi H., Kimchi A. (1997) DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death. Mol. Cell. Biol. 17(3):1615-1625
Shaughnessy JD J. r., Jenkins N. A., Copeland N. G. (1997) cDNA cloning, expression analysis, and chromosomal localization of a gene with high homology to wheat eIF-(iso)4F and mammalian eIF-4G. Genomics. 39(2):192-197
IRESs:
IRES:
Version: 6 Last change: 2009-03-22 23:40:09
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The IRES name:
  DAP5
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  112-416
Conclusion:
  putative_IRES
How IRES boundaries were determined:
experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
This IRES region is located on the NM_001418.1 sequence between positions 1-305.
Citations:
Henis-Korenblit S., Strumpf N. L., Goldstaub D., Kimchi A. (2000) A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation. Mol. Cell. Biol. 20(2):496-506
RNA:protein interactions:
The RNA:protein interaction:
Version: 1 Last change: 2006-10-28 00:00:00
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The description of the protein interacting with the RNA:
DAP5/p86
The organism where this RNA:protein interaction occurs:
Homo sapiens
Remarks:
DAP5/p86 is a caspase cleaved DAP5 protein form. It has been shown that transient over-expression of the
DAP5/p86 truncated protein form supports internal ribosome entry site-mediated translation of DAP5, Apaf-1,
c-Myc and XIAP (so-called death proteins).
Citations:
Henis-Korenblit S., Shani G., Sines T., Marash L., Shohat G., Kimchi A. (2002) The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins. Proc. Natl. Acad. Sci. U. S. A. 99(8):5400-5405
Nevins T. A., Harder Z. M., Korneluk R. G., Holcik M. (2003) Distinct regulation of internal ribosome entry site-mediated translation following cellular stress is mediated by apoptotic fragments of eIF4G translation initiation factor family members eIF4GI and p97/DAP5/NAT1. J. Biol. Chem. 278(6):3572-3579
The RNA:protein interaction:
Version: 1 Last change: 2006-10-28 00:00:00
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The description of the protein interacting with the RNA:
DAP5
The organism where this RNA:protein interaction occurs:
Homo sapiens SKW 6.4 (ATCC TIB-215)
Remarks:
The DAP5 protein mediates DAP5-IRES driven translation in vitro (in human SKW B-lymphoma cells).
Citations:
Henis-Korenblit S., Strumpf N. L., Goldstaub D., Kimchi A. (2000) A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation. Mol. Cell. Biol. 20(2):496-506
The RNA:protein interaction:
Version: 2 Last change: 2009-03-22 23:31:23
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The description of the protein interacting with the RNA:
DAP5
The organism where this RNA:protein interaction occurs:
Mus musculus
Remarks:
Direct binding of the DAP5 protein to DAP5 IRES was not demonstrated. DAP5 is not necessary for DAP5 IRES
driven translation in vivo (in mouse).
Citations:
Yamanaka S., Zhang X. Y., Maeda M., Miura K., Wang S., Farese RV J. r., Iwao H., Innerarity T. L. (2000) Essential role of NAT1/p97/DAP5 in embryonic differentiation and the retinoic acid pathway. EMBO J. 19(20):5533-5541
Last change to the database: 2015-04-16 16:45:23 GMT+1