The nucleic acid data:
IRESite Id: 122 Version: 7
Originaly submitted by: Václav Vopálenský
Reviewed by: Václav Vopálenský Last change: 2007-07-24 00:00:00
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  nonexisting_chimera_of_GenBank_record_and_tested_IRES_fragment
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  FMR1
The genetic origin of this natural mRNA/+RNA:
  nuclear
The GenBankId GI:# number of the most similar mRNA/+RNA sequence to this one.
50053960 
Synonyms of the gene name:
Synonym: FMRP
Synonym: FRAXA
Synonym: MGC87458
The mRNA/+RNA description: 
Homo sapiens fragile X mental retardation 1 (FMR1), mRNA.
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  reverse_engineered_sequence_and_should_match_experiment_except_3UTR
The organism containing this mRNA with IRES segment in its genome:
Homo sapiens
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
  1
Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:
Summary of experiments studying integrity of the in vivo transcripts in a particular host:
Integrity (uniformity) of mRNA tested using Northern-blot:
heterogeneous_population_of_molecules_found
Integrity (uniformity) of mRNA tested using RNase protection:
not_tested
Integrity (uniformity) of mRNA tested using 5'-RACE:
not_tested
Integrity (uniformity) of mRNA tested using primer extension :
not_tested
Integrity (uniformity) of mRNA tested using RT-PCR:
not_tested
Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR):
not_tested
Integrity (uniformity) of mRNA tested using RNAi:
not_tested
Integrity (uniformity) of mRNA tested using S1 nuclease mapping:
not_tested
Cryptic promoter presence was confirmed by expression from a promoter-less plasmid:
not_tested
Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter:
not_tested
Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks:
not_tested
The organism used:
Homo sapiens SK-N-MC (ATCC HTB-10)
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 0
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The abbreviated name of this ORF/gene:
FMR1
The description of the protein encoded in this ORF:
fragile X mental retardation 1
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  no
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  252-2153
OPTIONAL: The function of the encoded protein
RNA-binding protein. Associated to polysomes and might be involved in the transport of mRNA from the nucleus
to the cytoplasm.

GO:0005737;  Cellular component: cytoplasm
GO:0005730;  Cellular component: nucleolus
GO:0005654;  Cellular component: nucleoplasm
GO:0005625;  Cellular component: soluble fraction
GO:0003729;  Molecular function: mRNA binding
GO:0005515;  Molecular function: protein binding
Remarks:
This sequence is composed of Figure 1 (L29074) in cited article (5' UTR) and GenBank sequence number 50053960
(the rest of the mRNA) by curator [it does not start at the base 251 but at the base 252].
Citations:
Chiang P. W., Carpenter L. E., Hagerman P. J. (2001) The 5'-untranslated region of the FMR1 message facilitates translation by internal ribosome entry. J. Biol. Chem. 276(41):37916-37921
IRESs:
IRES:
Version: 5 Last change: 2007-07-24 00:00:00
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The IRES name:
  FMR1
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  1-252
Conclusion:
  putative_IRES
How IRES boundaries were determined:
experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
Sequences between -223 and -209 [bases 29 to 43 here in database] appear to contribute most of the observed
IRES activity of the FMR1 5'-UTR in the dicistronic context. This sequence is located mainly within a
21-nucleotide, pyrimidine-rich region spanning nucleotides -232 through -212 [bases 20 to 40 here in
database].
Citations:
Chiang P. W., Carpenter L. E., Hagerman P. J. (2001) The 5'-untranslated region of the FMR1 message facilitates translation by internal ribosome entry. J. Biol. Chem. 276(41):37916-37921
Last change to the database: 2015-04-16 16:45:23 GMT+1