IRESite_Id:126 negative control plasmid pFGAL4-L428

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `126`	Version: `2`
Originaly submitted by: Tomáš Mašek
Reviewed by: Tomáš Mašek	Last change: `2008-07-04 13:02:03`

IRESite record type:

negative_control_plasmid_with_promoter_and_without_putative_IRES

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
both_UTRs_incomplete

The mRNA/+RNA description:

mRNA produced by control plasmid pFGAL4-L428 which comprises luciferase and Gal4 ORFs. L428 clone
was selected from pFGAL4::lambda library containing randomly cleaved phage lambda DNA for its comparable
length  of intercistronic region to tested pFGAL4h-HCV1.

The mRNA/+RNA sequence represented in the +DNA notation:

ATGGAAGACG CCAAAAACAT AAAGAAAGGC CCGGCGCCAT TCTATCCGCT GGAAGATGGA ACCGCTGGAG AGCAACTGCA TAAGGCTATG AAGAGATACG 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

CCCTGGTTCC TGGAACAATT GCTTTTACAG ATGCACATAT CGAGGTGGAC ATCACTTACG CTGAGTACTT CGAAATGTCC GTTCGGTTGG CAGAAGCTAT 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

GAAACGATAT GGGCTGAATA CAAATCACAG AATCGTCGTA TGCAGTGAAA ACTCTCTTCA ATTCTTTATG CCGGTGTTGG GCGCGTTATT TATCGGAGTT 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

GCAGTTGCGC CCGCGAACGA CATTTATAAT GAACGTGAAT TGCTCAACAG TATGGGCATT TCGCAGCCTA CCGTGGTGTT CGTTTCCAAA AAGGGGTTGC 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

AAAAAATTTT GAACGTGCAA AAAAAGCTCC CAATCATCCA AAAAATTATT ATCATGGATT CTAAAACGGA TTACCAGGGA TTTCAGTCGA TGTACACGTT 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

CGTCACATCT CATCTACCTC CCGGTTTTAA TGAATACGAT TTTGTGCCAG AGTCCTTCGA TAGGGACAAG ACAATTGCAC TGATCATGAA CTCCTCTGGA 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

TCTACTGGTC TGCCTAAAGG TGTCGCTCTG CCTCATAGAA CTGCCTGCGT GAGATTCTCG CATGCCAGAG ATCCTATTTT TGGCAATCAA ATCATTCCGG 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

ATACTGCGAT TTTAAGTGTT GTTCCATTCC ATCACGGTTT TGGAATGTTT ACTACACTCG GATATTTGAT ATGTGGATTT CGAGTCGTCT TAATGTATAG 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ATTTGAAGAA GAGCTGTTTC TGAGGAGCCT TCAGGATTAC AAGATTCAAA GTGCGCTGCT GGTGCCAACC CTATTCTCCT TCTTCGCCAA AAGCACTCTG 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

ATTGACAAAT ACGATTTATC TAATTTACAC GAAATTGCTT CTGGTGGCGC TCCCCTCTCT AAGGAAGTCG GGGAAGCGGT TGCCAAGAGG TTCCATCTGC 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

CAGGTATCAG GCAAGGATAT GGGCTCACTG AGACTACATC AGCTATTCTG ATTACACCCG AGGGGGATGA TAAACCGGGC GCGGTCGGTA AAGTTGTTCC 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

ATTTTTTGAA GCGAAGGTTG TGGATCTGGA TACCGGGAAA ACGCTGGGCG TTAATCAAAG AGGCGAACTG TGTGTGAGAG GTCCTATGAT TATGTCCGGT 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

TATGTAAACA ATCCGGAAGC GACCAACGCC TTGATTGACA AGGATGGATG GCTACATTCT GGAGACATAG CTTACTGGGA CGAAGACGAA CACTTCTTCA 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

TCGTTGACCG CCTGAAGTCT CTGATTAAGT ACAAAGGCTA TCAGGTGGCT CCCGCTGAAT TGGAATCCAT CTTGCTCCAA CACCCCAACA TCTTCGACGC 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

AGGTGTCGCA GGTCTTCCCG ACGATGACGC CGGTGAACTT CCCGCCGCCG TTGTTGTTTT GGAGCACGGA AAGACGATGA CGGAAAAAGA GATCGTGGAT 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

TACGTCGCCA GTCAAGTAAC AACCGCGAAA AAGTTGCGCG GAGGAGTTGT GTTTGTGGAC GAAGTACCGA AAGGTCTTAC CGGAAAACTC GACGCAAGAA 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

AAATCAGAGA GATCCTCATA AAGGCCAAGA AGGGCGGAAA GATCGCCGTG TAATTCTAGC CTAGGGCCCA CAAGCTTGGC TTGGAGCCTG TTGGTGCGGT 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

CATGGAATTA TGCGGAACAG AATCATGCCT GGTGTTTACA TAGTAATAAT TTGTGAGAGA TAGACTTTCT CCGTGGTGAA GGGATAGCTC TCACGGAAAA 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

AGAGACGCAG AAACAGCGGA TCAAACTTAA ATTGCATCTA CTCGTCGCGA ACCGCTTTCA TCCGGCACAG TATCAAGGTA TTTTATGCGC GCACGAAAAG 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

CATCAGGTCT TTCCTTCGAA GGGGATCCGC CTACCTTTCA CGAGTTGCGC AGTTTGTCTG CAAGACTCTA TGAGAAGCAG ATAAGCGATA AGTTTGCTCA 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

ACATCTTCTC GGGCATAAGT CGGACACCAT GGCATCACAG TATCGTGATG ACAGAGGCAG GGAGTGGGAC AAAATTCAAA AATGAAGCTA CTGTCTTCTA 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

TCGAACAAGC ATGCGATATT TGCCGACTTA AAAAGCTCAA GTGCTCCAAA GAAAAACCGA AGTGCGCCAA GTGTCTGAAG AACAACTGGG AGTGTCGCTA 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

CTCTCCCAAA ACCAAAAGGT CTCCGCTGAC TAGGGCACAT CTGACAGAAG TGGAATCAAG GCTAGAAAGA CTGGAACAGC TATTTCTACT GATTTTTCCT 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

CGAGAAGACC TTGACATGAT TTTGAAAATG GATTCTTTAC AGGATATAAA AGCATTGTTA ACAGGATTAT TTGTACAAGA TAATGTGAAT AAAGATGCCG 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

TCACAGATAG ATTGGCTTCA GTGGAGACTG ATATGCCTCT AACATTGAGA CAGCATAGAA TAAGTGCGAC ATCATCATCG GAAGAGAGTA GTAACAAAGG 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

TCAAAGACAG TTGACTGTAT CGATTGACTC GGCAGCTCAT CATGATAACT CCACAATTCC GTTGGATTTT ATGCCCAGGG ATGCTCTTCA TGGATTTGAT 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

TGGTCTGAAG AGGATGACAT GTCGGATGGC TTGCCCTTCC TGAAAACGGA CCCCAACAAT AATGGGTTCT TTGGCGACGG TTCTCTCTTA TGTATTCTTC 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

GATCTATTGG CTTTAAACCG GAAAATTACA CGAACTCTAA CGTTAACAGG CTCCCGACCA TGATTACGGA TAGATACACG TTGGCTTCTA GATCCACAAC 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

ATCCCGTTTA CTTCAAAGTT ATCTCAATAA TTTTCACCCC TACTGCCCTA TCGTGCACTC ACCGACGCTA ATGATGTTGT ATAATAACCA GATTGAAATC 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

GCGTCGAAGG ATCAATGGCA AATCCTTTTT AACTGCATAT TAGCCATTGG AGCCTGGTGT ATAGAGGGGG AATCTACTGA TATAGATGTT TTTTACTATC 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

AAAATGCTAA ATCTCATTTG ACGAGCAAGG TCTTCGAGTC AGGTTCCATA ATTTTGGTGA CAGCCCTACA TCTTCTGTCG CGATATACAC AGTGGAGGCA 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

GAAAACAAAT ACTAGCTATA ATTTTCACAG CTTTTCCATA AGAATGGCCA TATCATTGGG CTTGAATAGG GACCTCCCCT CGTCCTTCAG TGATAGCAGC 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

ATTCTGGAAC AAAGACGCCG AATTTGGTGG TCTGTCTACT CTTGGGAGAT CCAATTGTCC CTGCTTTATG GTCGATCCAT CCAGCTTTCT CAGAATACAA 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

TCTCCTTCCC TTCTTCTGTC GACGATGTGC AGCGTACCAC AACAGGTCCC ACCATATATC ATGGCATCAT TGAAACAGCA AGGCTCTTAC AAGTTTTCAC 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

AAAAATCTAT GAACTAGACA AAACAGTAAC TGCAGAAAAA AGTCCTATAT GTGCAAAAAA ATGCTTGATG ATTTGTAATG AGATTGAGGA GGTTTCGAGA 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

CAGGCACCAA AGTTTTTACA AATGGATATT TCCACCACCG CTCTAACCAA TTTGTTGAAG GAACACCCTT GGCTATCCTT TACAAGATTC GAACTGAAGT 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

GGAAACAGTT GTCTCTTATC ATTTATGTAT TAAGAGATTT TTTCACTAAT TTTACCCAGA AAAAGTCACA ACTAGAACAG GATCAAAATG ATCATCAAAG 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

TTATGAAGTT AAACGATGCT CCATCATGTT AAGCGATGCA GCACAAAGAA CTGTTATGTC TGTAAGTAGC TATATGGACA ATCATAATGT CACCCCATAT 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

TTTGCCTGGA ATTGTTCTTA TTACTTGTTC AATGCAGTCC TAGTACCCAT AAAGACTCTA CTCTCAAACT CAAAATCGAA TGCTGAGAAT AACGAGACCG 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

CACAATTATT ACAACAAATT AACACTGTTC TGATGCTATT AAAAAAACTG GCCACTTTTA AAATCCAGAC TTGTGAAAAA TACATTCAAG TACTGGAAGA 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

GGTATGTGCG CCGTTTCTGT TATCACAGTG TGCAATCCCA TTACCGCATA TCAGTTATAA CAATAGTAAT GGTAGCGCCA TTAAAAATAT TGTCGGTTCT 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

GCAACTATCG CCCAATACCC TACTCTTCCG GAGGAAAATG TCAACAATAT CAGTGTTAAA TATGTTTCTC CTGGCTCAGT AGGGCCTTCA CCTGTGCCAT 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

TGAAATCAGG AGCAAGTTTC AGTGATCTAG TCAAGCTGTT ATCTAACCGT CCACCCTCTC GTAACTCTCC AGTGACAATA CCAAGAAGCA CACCTTCGCA 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

TCGCTCAGTC ACGCCTTTTC TAGGGCAACA GCAACAGCTG CAATCATTAG TGCCACTGAC CCCGTCTGCT TTGTTTGGTG GCGCCAATTT TAATCAAAGT 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

GGGAATATTG CTGATAGCTC ATTGTCCTTC ACTTTCACTA ACAGTAGCAA CGGTCCGAAC CTCATAACAA CTCAAACAAA TTCTCAAGCG CTTTCACAAC 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

CAATTGCCTC CTCTAACGTT CATGATAACT TCATGAATAA TGAAATCACG GCTAGTAAAA TTGATGATGG TAATAATTCA AAACCACTGT CACCTGGTTG 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

GACGGACCAA ACTGCGTATA ACGCGTTTGG AATCACTACA GGGATGTTTA ATACCACTAC AATGGATGAT GTATATAACT ATCTATTCGA TGATGAAGAT 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

ACCCCACCAA ACCCAAAAAA AGAGTAA
^4701      ^4711      ^4721

Credibility of mRNA sequence:

end-to-end_sequence_completely_same_as_in_the_experiment

The name of the plasmid:
pFGAL4-L428 The name of the promoter used to express this mRNA:
TPI

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

GAATTAATTC ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT ACGCTGAGTA CTTCGAAATG TCCGTTCGGT 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

TGGCAGAAGC TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT TGGGCGCGTT 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTGC CAGAGTCCTT CGATAGGGAC AAGACAATTG CACTGATCAT 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

GAACTCCTCT GGATCTACTG GTCTGCCTAA AGGTGTCGCT CTGCCTCATA GAACTGCCTG CGTGAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

CAAATCATTC CGGATACTGC GATTTTAAGT GTTGTTCCAT TCCATCACGG TTTTGGAATG TTTACTACAC TCGGATATTT GATATGTGGA TTTCGAGTCG 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTCTGAGGAG CCTTCAGGAT TACAAGATTC AAAGTGCGCT GCTGGTGCCA ACCCTATTCT CCTTCTTCGC 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG CTTCTGGTGG CGCTCCCCTC TCTAAGGAAG TCGGGGAAGC GGTTGCCAAG 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

AGGTTCCATC TGCCAGGTAT CAGGCAAGGA TATGGGCTCA CTGAGACTAC ATCAGCTATT CTGATTACAC CCGAGGGGGA TGATAAACCG GGCGCGGTCG 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT GGATACCGGG AAAACGCTGG GCGTTAATCA AAGAGGCGAA CTGTGTGTGA GAGGTCCTAT 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG ACAAGGATGG ATGGCTACAT TCTGGAGACA TAGCTTACTG GGACGAAGAC 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

GAACACTTCT TCATCGTTGA CCGCCTGAAG TCTCTGATTA AGTACAAAGG CTATCAGGTG GCTCCCGCTG AATTGGAATC CATCTTGCTC CAACACCCCA 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

ACATCTTCGA CGCAGGTGTC GCAGGTCTTC CCGACGATGA CGCCGGTGAA CTTCCCGCCG CCGTTGTTGT TTTGGAGCAC GGAAAGACGA TGACGGAAAA 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

AGAGATCGTG GATTACGTCG CCAGTCAAGT AACAACCGCG AAAAAGTTGC GCGGAGGAGT TGTGTTTGTG GACGAAGTAC CGAAAGGTCT TACCGGAAAA 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

CTCGACGCAA GAAAAATCAG AGAGATCCTC ATAAAGGCCA AGAAGGGCGG AAAGATCGCC GTGTAATTCT AGCCTAGGGC CCACAAGCTT GGCTTGGAGC 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

CTGTTGGTGC GGTCATGGAA TTATGCGGAA CAGAATCATG CCTGGTGTTT ACATAGTAAT AATTTGTGAG AGATAGACTT TCTCCGTGGT GAAGGGATAG 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

CTCTCACGGA AAAAGAGACG CAGAAACAGC GGATCAAACT TAAATTGCAT CTACTCGTCG CGAACCGCTT TCATCCGGCA CAGTATCAAG GTATTTTATG 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

CGCGCACGAA AAGCATCAGG TCTTTCCTTC GAAGGGGATC CGCCTACCTT TCACGAGTTG CGCAGTTTGT CTGCAAGACT CTATGAGAAG CAGATAAGCG 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

ATAAGTTTGC TCAACATCTT CTCGGGCATA AGTCGGACAC CATGGCATCA CAGTATCGTG ATGACAGAGG CAGGGAGTGG GACAAAATTC AAAAATGAAG 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

CTACTGTCTT CTATCGAACA AGCATGCGAT ATTTGCCGAC TTAAAAAGCT CAAGTGCTCC AAAGAAAAAC CGAAGTGCGC CAAGTGTCTG AAGAACAACT 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

GGGAGTGTCG CTACTCTCCC AAAACCAAAA GGTCTCCGCT GACTAGGGCA CATCTGACAG AAGTGGAATC AAGGCTAGAA AGACTGGAAC AGCTATTTCT 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

ACTGATTTTT CCTCGAGAAG ACCTTGACAT GATTTTGAAA ATGGATTCTT TACAGGATAT AAAAGCATTG TTAACAGGAT TATTTGTACA AGATAATGTG 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

AATAAAGATG CCGTCACAGA TAGATTGGCT TCAGTGGAGA CTGATATGCC TCTAACATTG AGACAGCATA GAATAAGTGC GACATCATCA TCGGAAGAGA 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

GTAGTAACAA AGGTCAAAGA CAGTTGACTG TATCGATTGA CTCGGCAGCT CATCATGATA ACTCCACAAT TCCGTTGGAT TTTATGCCCA GGGATGCTCT 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

TCATGGATTT GATTGGTCTG AAGAGGATGA CATGTCGGAT GGCTTGCCCT TCCTGAAAAC GGACCCCAAC AATAATGGGT TCTTTGGCGA CGGTTCTCTC 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

TTATGTATTC TTCGATCTAT TGGCTTTAAA CCGGAAAATT ACACGAACTC TAACGTTAAC AGGCTCCCGA CCATGATTAC GGATAGATAC ACGTTGGCTT 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

CTAGATCCAC AACATCCCGT TTACTTCAAA GTTATCTCAA TAATTTTCAC CCCTACTGCC CTATCGTGCA CTCACCGACG CTAATGATGT TGTATAATAA 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

CCAGATTGAA ATCGCGTCGA AGGATCAATG GCAAATCCTT TTTAACTGCA TATTAGCCAT TGGAGCCTGG TGTATAGAGG GGGAATCTAC TGATATAGAT 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

GTTTTTTACT ATCAAAATGC TAAATCTCAT TTGACGAGCA AGGTCTTCGA GTCAGGTTCC ATAATTTTGG TGACAGCCCT ACATCTTCTG TCGCGATATA 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

CACAGTGGAG GCAGAAAACA AATACTAGCT ATAATTTTCA CAGCTTTTCC ATAAGAATGG CCATATCATT GGGCTTGAAT AGGGACCTCC CCTCGTCCTT 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

CAGTGATAGC AGCATTCTGG AACAAAGACG CCGAATTTGG TGGTCTGTCT ACTCTTGGGA GATCCAATTG TCCCTGCTTT ATGGTCGATC CATCCAGCTT 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

TCTCAGAATA CAATCTCCTT CCCTTCTTCT GTCGACGATG TGCAGCGTAC CACAACAGGT CCCACCATAT ATCATGGCAT CATTGAAACA GCAAGGCTCT 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

TACAAGTTTT CACAAAAATC TATGAACTAG ACAAAACAGT AACTGCAGAA AAAAGTCCTA TATGTGCAAA AAAATGCTTG ATGATTTGTA ATGAGATTGA 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

GGAGGTTTCG AGACAGGCAC CAAAGTTTTT ACAAATGGAT ATTTCCACCA CCGCTCTAAC CAATTTGTTG AAGGAACACC CTTGGCTATC CTTTACAAGA 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

TTCGAACTGA AGTGGAAACA GTTGTCTCTT ATCATTTATG TATTAAGAGA TTTTTTCACT AATTTTACCC AGAAAAAGTC ACAACTAGAA CAGGATCAAA 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

ATGATCATCA AAGTTATGAA GTTAAACGAT GCTCCATCAT GTTAAGCGAT GCAGCACAAA GAACTGTTAT GTCTGTAAGT AGCTATATGG ACAATCATAA 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

TGTCACCCCA TATTTTGCCT GGAATTGTTC TTATTACTTG TTCAATGCAG TCCTAGTACC CATAAAGACT CTACTCTCAA ACTCAAAATC GAATGCTGAG 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

AATAACGAGA CCGCACAATT ATTACAACAA ATTAACACTG TTCTGATGCT ATTAAAAAAA CTGGCCACTT TTAAAATCCA GACTTGTGAA AAATACATTC 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

AAGTACTGGA AGAGGTATGT GCGCCGTTTC TGTTATCACA GTGTGCAATC CCATTACCGC ATATCAGTTA TAACAATAGT AATGGTAGCG CCATTAAAAA 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

TATTGTCGGT TCTGCAACTA TCGCCCAATA CCCTACTCTT CCGGAGGAAA ATGTCAACAA TATCAGTGTT AAATATGTTT CTCCTGGCTC AGTAGGGCCT 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

TCACCTGTGC CATTGAAATC AGGAGCAAGT TTCAGTGATC TAGTCAAGCT GTTATCTAAC CGTCCACCCT CTCGTAACTC TCCAGTGACA ATACCAAGAA 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

GCACACCTTC GCATCGCTCA GTCACGCCTT TTCTAGGGCA ACAGCAACAG CTGCAATCAT TAGTGCCACT GACCCCGTCT GCTTTGTTTG GTGGCGCCAA 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

TTTTAATCAA AGTGGGAATA TTGCTGATAG CTCATTGTCC TTCACTTTCA CTAACAGTAG CAACGGTCCG AACCTCATAA CAACTCAAAC AAATTCTCAA 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

GCGCTTTCAC AACCAATTGC CTCCTCTAAC GTTCATGATA ACTTCATGAA TAATGAAATC ACGGCTAGTA AAATTGATGA TGGTAATAAT TCAAAACCAC 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

TGTCACCTGG TTGGACGGAC CAAACTGCGT ATAACGCGTT TGGAATCACT ACAGGGATGT TTAATACCAC TACAATGGAT GATGTATATA ACTATCTATT 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

CGATGATGAA GATACCCCAC CAAACCCAAA AAAAGAGTAA GAGCTCAGCT AGCTAACTGA ATAAGGAACA ATGAACGTTT TTCCTTTCTC TTGTTCCTAG 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

TATTAATGAC TGACCGATAC ATCCCTTTTT TTTTTTGTCT TTGTCTAGCT CCAATTCGCC CTATAGTGAG TCGTATTACA ATTCACTGGC CGTCGTTTTA 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTCCT 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAG GGTAATAACT GATATAATTA AATTGAAGCT CTAATTTGTG AGTTTAGTAT 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

ACATGCATTT ACTTATAATA CAGTTTTTTA GTTTTGCTGG CCGCATCTTC TCAAATATGC TTCCCAGCCT GCTTTTCTGT AACGTTCACC CTCTACCTTA 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

GCATCCCTTC CCTTTGCAAA TAGTCCTCTT CCAACAATAA TAATGTCAGA TCCTGTAGAG ACCACATCAT CCACGGTTCT ATACTGTTGA CCCAATGCGT 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

CTCCCTTGTC ATCTAAACCC ACACCGGGTG TCATAATCAA CCAATCGTAA CCTTCATCTC TTCCACCCAT GTCTCTTTGA GCAATAAAGC CGATAACAAA 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

ATCTTTGTCG CTCTTCGCAA TGTCAACAGT ACCCTTAGTA TATTCTCCAG TAGATAGGGA GCCCTTGCAT GACAATTCTG CTAACATCAA AAGGCCTCTA 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

GGTTCCTTTG TTACTTCTTC TGCCGCCTGC TTCAAACCGC TAACAATACC TGGCCCCACC ACACCGTGTG CATTCGTAAT GTCTGCCCAT TCTGCTATTC 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

TGTATACACC CGCAGAGTAC TGCAATTTGA CTGTATTACC AATGTCAGCA AATTTTCTGT CTTCGAAGAG TAAAAAATTG TACTTGGCGG ATAATGCCTT 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

TAGCGGCTTA ACTGTGCCCT CCATCGAAAA ATCAGTCAAT ATATCCACAT GTGTTTTTAG TAAACAAATT TTGGGACCTA ATGCTTCAAC TAACTCCAGT 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

AATTCCTTGG TGGTACGAAC ATCCAATGAA GCACACAAGT TTGTTTGCTT TTCGTGCATG ATATTAAATA GCTTGGCAGC AACAGGACTA GGATGAGTAG 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

CAGCACGTTC CTTATATGTA GCTTTCGACA TGATTTATCT TCGTTTCCTG CAGGTTTTTG TTCTGTGCAG TTGGGTTAAG AATACTGGGC AATTTCATGT 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

TTCTTCAACA CTACATATGC GTATATATAC CAATCTAAGT CTGTGCTCCT TCCTTCGTTC TTCCTTCTGT TCGGAGATTA CCGAATCAAA AAAATTTCAA 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

GGAAACCGAA ATCAAAAAAA AGAATAAAAA AAAAATGATG AATTGAAAAG GTGGTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

CTTAGACGTG CGGCCGCTCT AGAACTAGTG GATCAATTCC ACGGACTATA GACTATACTA GTATACTCCG TCTACTGTAC GATACACTTC CGCTCAGGTC 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

CTTGTCCTTT AACGAGGCCT TACCACTCTT TTGTTACTCT ATTGATCCAG CTCAGCAAAG GCAGTGTGAT CTAAGATTCT ATCTTCGCGA TGTAGTAAAA 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

CTAGCTAGAC CGAGAAAGAG ACTAGAAATG CAAAAGGCAC TTCTACAATG GCTGCCATCA TTATTATCCG ATGTGACGCT GCAGCTTCTC AATGATATTC 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

GAATACGCTT TGAGGAGATA CAGCCTAATA TCCGACAAAC TGTTTTACAG ATTTACGATC GTACTTGTTA CCCATCATTG AATTTTGAAC ATCCGAACCT 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

GGGAGTTTTC CCTGAAACAG ATAGTATATT TGAACCTGTA TAATAATATA TAGTCTAGCG CTTTACGGAA GACAATGTAT GTATTTCGGT TCCTGGAGAA 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

ACTATTGCAT CTATTGCATA GGTAATCTTG CACGTCGCAT CCCCGGTTCA TTTTCTGCGT TTCCATCTTG CACTTCAATA GCATATCTTT GTTAACGAAG 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

CATCTGTGCT TCATTTTGTA GAACAAAAAT GCAACGCGAG AGCGCTAATT TTTCAAACAA AGAATCTGAG CTGCATTTTT ACAGAACAGA AATGCAACGC 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

GAAAGCGCTA TTTTACCAAC GAAGAATCTG TGCTTCATTT TTGTAAAACA AAAATGCAAC GCGAGAGCGC TAATTTTTCA AACAAAGAAT CTGAGCTGCA 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

TTTTTACAGA ACAGAAATGC AACGCGAGAG CGCTATTTTA CCAACAAAGA ATCTATACTT CTTTTTTGTT CTACAAAAAT GCATCCCGAG AGCGCTATTT 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

TTCTAACAAA GCATCTTAGA TTACTTTTTT TCTCCTTTGT GCGCTCTATA ATGCAGTCTC TTGATAACTT TTTGCACTGT AGGTCCGTTA AGGTTAGAAG 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

AAGGCTACTT TGGTGTCTAT TTTCTCTTCC ATAAAAAAAG CCTGACTCCA CTTCCCGCGT TTACTGATTA CTAGCGAAGC TGCGGGTGCA TTTTTTCAAG 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

ATAAAGGCAT CCCCGATTAT ATTCTATACC GATGTGGATT GCGCATACTT TGTGAACAGA AAGTGATAGC GTTGATGATT CTTCATTGGT CAGAAAATTA 
^8001      ^8011      ^8021      ^8031      ^8041      ^8051      ^8061      ^8071      ^8081      ^8091      

TGAACGGTTT CTTCTATTTT GTCTCTATAT ACTACGTATA GGAAATGTTT ACATTTTCGT ATTGTTTTCG ATTCACTCTA TGAATAGTTC TTACTACAAT 
^8101      ^8111      ^8121      ^8131      ^8141      ^8151      ^8161      ^8171      ^8181      ^8191      

TTTTTTGTCT AAAGAGTAAT ACTAGAGATA AACATAAAAA ATGTAGAGGT CGAGTTTAGA TGCAAGTTCA AGGAGCGAAA GGTGGATGGG TAGGTTATAT 
^8201      ^8211      ^8221      ^8231      ^8241      ^8251      ^8261      ^8271      ^8281      ^8291      

AGGGATATAG CACAGAGATA TATAGCAAAG AGATACTTTT GAGCAATGTT TGTGGAAGCG GTATTCGCAA TATTTTAGTA GCTCGTTACA GTCCGGTGCG 
^8301      ^8311      ^8321      ^8331      ^8341      ^8351      ^8361      ^8371      ^8381      ^8391      

TTTTTGGTTT TTTGAAAGTG CGTCTTCAGA GCGCTTTTGG TTTTCAAAAG CGCTCTGAAG TTCCTATACT TTCTAGAGAA TAGGAACTTC GGAATAGGAA 
^8401      ^8411      ^8421      ^8431      ^8441      ^8451      ^8461      ^8471      ^8481      ^8491      

CTTCAAAGCG TTTCCGAAAA CGAGCGCTTC CGAAAATGCA ACGCGAGCTG CGCACATACA GCTCACTGTT CACGTCGCAC CTATATCTGC GTGTTGCCTG 
^8501      ^8511      ^8521      ^8531      ^8541      ^8551      ^8561      ^8571      ^8581      ^8591      

TATATATATA TACATGAGAA GAACGGCATA GTGCGTGTTT ATGCTTAAAT GCGTACTTAT ATGCGTCTAT TTATGTAGGA TGAAAGGTAG TCTAGTACCT 
^8601      ^8611      ^8621      ^8631      ^8641      ^8651      ^8661      ^8671      ^8681      ^8691      

CCTGTGATAT TATCCCATTC CATGCGGGGT ATCGTATGCT TCCTTCAGCA CTACCCTTTA GCTGTTCTAT ATGCTGCCAC TCCTCAATTG GATTAGTCTC 
^8701      ^8711      ^8721      ^8731      ^8741      ^8751      ^8761      ^8771      ^8781      ^8791      

ATCCTTCAAT GCTATCATTT CCTTTGATAT TGGATCATAT GCATAGTACC GAGAAACTAG TGCGAAGTAG TGATCAGGTA TTGCTGTTAT CTGATGAGTA 
^8801      ^8811      ^8821      ^8831      ^8841      ^8851      ^8861      ^8871      ^8881      ^8891      

TACGTTGTCC TGGCCACGGC AGAAGCACGC TTATCGCTCC AATTTCCCAC AACATTAGTC AACTCCGTTA GGCCCTTCAT TGAAAGAAAT GAGGTCATCA 
^8901      ^8911      ^8921      ^8931      ^8941      ^8951      ^8961      ^8971      ^8981      ^8991      

AATGTCTTCC AATGTGAGAT TTTGGGCCAT TTTTTATAGC AAAGATTGAA TAAGGCGCAT TTTTCTTCAA AGCTGCGGCC GCACTCTCAC TAGTACGTCA 
^9001      ^9011      ^9021      ^9031      ^9041      ^9051      ^9061      ^9071      ^9081      ^9091      

GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA 
^9101      ^9111      ^9121      ^9131      ^9141      ^9151      ^9161      ^9171      ^9181      ^9191      

TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA 
^9201      ^9211      ^9221      ^9231      ^9241      ^9251      ^9261      ^9271      ^9281      ^9291      

CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 
^9301      ^9311      ^9321      ^9331      ^9341      ^9351      ^9361      ^9371      ^9381      ^9391      

TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC 
^9401      ^9411      ^9421      ^9431      ^9441      ^9451      ^9461      ^9471      ^9481      ^9491      

GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC 
^9501      ^9511      ^9521      ^9531      ^9541      ^9551      ^9561      ^9571      ^9581      ^9591      

CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 
^9601      ^9611      ^9621      ^9631      ^9641      ^9651      ^9661      ^9671      ^9681      ^9691      

ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC 
^9701      ^9711      ^9721      ^9731      ^9741      ^9751      ^9761      ^9771      ^9781      ^9791      

TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC 
^9801      ^9811      ^9821      ^9831      ^9841      ^9851      ^9861      ^9871      ^9881      ^9891      

GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 
^9901      ^9911      ^9921      ^9931      ^9941      ^9951      ^9961      ^9971      ^9981      ^9991      

GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC 
^10001     ^10011     ^10021     ^10031     ^10041     ^10051     ^10061     ^10071     ^10081     ^10091     

AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC 
^10101     ^10111     ^10121     ^10131     ^10141     ^10151     ^10161     ^10171     ^10181     ^10191     

TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 
^10201     ^10211     ^10221     ^10231     ^10241     ^10251     ^10261     ^10271     ^10281     ^10291     

ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA 
^10301     ^10311     ^10321     ^10331     ^10341     ^10351     ^10361     ^10371     ^10381     ^10391     

ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 
^10401     ^10411     ^10421     ^10431     ^10441     ^10451     ^10461     ^10471     ^10481     ^10491     

TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 
^10501     ^10511     ^10521     ^10531     ^10541     ^10551     ^10561     ^10571     ^10581     ^10591     

CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC 
^10601     ^10611     ^10621     ^10631     ^10641     ^10651     ^10661     ^10671     ^10681     ^10691     

CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 
^10701     ^10711     ^10721     ^10731     ^10741     ^10751     ^10761     ^10771     ^10781     ^10791     

TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 
^10801     ^10811     ^10821     ^10831     ^10841     ^10851     ^10861     ^10871     ^10881     ^10891     

GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 
^10901     ^10911     ^10921     ^10931     ^10941     ^10951     ^10961     ^10971     ^10981     ^10991     

GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT 
^11001     ^11011     ^11021     ^11031     ^11041     ^11051     ^11061     ^11071     ^11081     ^11091     

TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 
^11101     ^11111     ^11121     ^11131     ^11141     ^11151     ^11161     ^11171     ^11181     ^11191     

TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCG AAATTAACCC TCACTAAAGG GAACAAAAGC 
^11201     ^11211     ^11221     ^11231     ^11241     ^11251     ^11261     ^11271     ^11281     ^11291     

TGGTACCGGG CCGGCCGTCG AGCTTGATGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 
^11301     ^11311     ^11321     ^11331     ^11341     ^11351     ^11361     ^11371     ^11381     ^11391     

ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
^11401     ^11411     ^11421     ^11431     ^11441     ^11451     ^11461     ^11471     ^11481     ^11491     

CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 
^11501     ^11511     ^11521     ^11531     ^11541     ^11551     ^11561     ^11571     ^11581     ^11591     

ACCGAGTTGC TCTTGCCCGG CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 
^11601     ^11611     ^11621     ^11631     ^11641     ^11651     ^11661     ^11671     ^11681     ^11691     

CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
^11701     ^11711     ^11721     ^11731     ^11741     ^11751     ^11761     ^11771     ^11781     ^11791     

GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 
^11801     ^11811     ^11821     ^11831     ^11841     ^11851     ^11861     ^11871     ^11881     ^11891     

TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
^11901     ^11911     ^11921     ^11931     ^11941     ^11951     ^11961     ^11971     ^11981     ^11991     

GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCAAGAATT GGGGATCTAC GTATGGTCAT 
^12001     ^12011     ^12021     ^12031     ^12041     ^12051     ^12061     ^12071     ^12081     ^12091     

TTCTTCTTCA GATTCCCTCA TGGAGAAAGT GCGGCAGATG TATATGACAG AGTCGCCAGT TTCCAAGAGA CTTTATTCAG GCACTTCCAT GATAGGCAAG 
^12101     ^12111     ^12121     ^12131     ^12141     ^12151     ^12161     ^12171     ^12181     ^12191     

AGAGAAGACC CAGAGATGTT GTTGTCCTAG TTACACATGG TATTTATTCC AGAGTATTCC TGATGAAATG GTTTAGATGG ACATACGAAG AGTTTGAATC 
^12201     ^12211     ^12221     ^12231     ^12241     ^12251     ^12261     ^12271     ^12281     ^12291     

GTTTACCAAT GTTCCTAACG GGAGCGTAAT GGTGATGGAA CTGGACGAAT CCATCAATAG ATACGTCCTG AGGACCGTGC TACCCAAATG GACTGATTGT 
^12301     ^12311     ^12321     ^12331     ^12341     ^12351     ^12361     ^12371     ^12381     ^12391     

GAGGGAGACC TAACTACATA GTGTTTAAAG ATTACGGATA TTTAACTTAC TTAGAATAAT GCCATTTTTT TGAGTTATAA TAATCCTACG TTAGTGTGAG 
^12401     ^12411     ^12421     ^12431     ^12441     ^12451     ^12461     ^12471     ^12481     ^12491     

CGGGATTTAA ACTGTGAGGA CCTTAATACA TTCAGACACT TCTGCGGTAT CACCCTACTT ATTCCCTTCG AGATTATATC TAGGAACCCA TCAGGTTGGT 
^12501     ^12511     ^12521     ^12531     ^12541     ^12551     ^12561     ^12571     ^12581     ^12591     

GGAAGATTAC CCGTTCTAAG ACTTTTCAGC TTCCTCTATT GATGTTACAC CTGGACACCC CTTTTCTGGC ATCCAGTTTT TAATCTTCAG TGGCATGTGA 
^12601     ^12611     ^12621     ^12631     ^12641     ^12651     ^12661     ^12671     ^12681     ^12691     

GATTCTCCGA AATTAATTAA AGCAATCACA CAATTCTCTC GGATACCACC TCGGTTGAAA CTGACAGGTG GTTTGTTACG CATGCTAATG CAAAGGAGCC 
^12701     ^12711     ^12721     ^12731     ^12741     ^12751     ^12761     ^12771     ^12781     ^12791     

TATATACCTT TGGCTCGGCT GCTGTAACAG GGAATATAAA GGGCAGCATA ATTTAGGAGT TTAGTGAACT TGCAACATTT ACTATTTTCC CTTCTTACGT 
^12801     ^12811     ^12821     ^12831     ^12841     ^12851     ^12861     ^12871     ^12881     ^12891     

AAATATTTTT CTTTTTAATT CTAAATCAAT CTTTTTCAAT TTTTTGTTTG TATTCTTTTC TTGCTTAAAT CTATAACTAC AAAAAACACA TACAG
^12901     ^12911     ^12921     ^12931     ^12941     ^12951     ^12961     ^12971     ^12981     ^12991

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

Plasmid sequence verified (partially/completely) by IRESite (more details in Remarks):

plasmid sequence confirmed by IRESite curators by restriction analysis + parts by PCR + sequencing

A promoter reported in cDNA corresponding to IRES sequence:
not tested The total number of notable open-reading frames (ORFs):
2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Tomáš Mašek	Reviewed by: Tomáš Mašek

The abbreviated name of this ORF/gene:
Fluc

The description of the protein encoded in this ORF:
firefly luciferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

no

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1-1653

ORF

ORF position:

2

Version: `0`
Originaly submitted by: Tomáš Mašek	Reviewed by: Tomáš Mašek

The abbreviated name of this ORF/gene:
GAL4

The description of the protein encoded in this ORF:
DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

no

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

2082-4727

OPTIONAL: The function of the encoded protein

Molecular Function: transcriptional activator activity (TAS)
Biological Process: galactose metabolism (TAS) regulation of transcription, DNA-dependent (TAS)
Cellular Component: nucleus (TAS)

Remarks:

L428 sequence was inserted into intercistronic position of pFGAL4 bicistronic plasmid to create bicistronic
control vector that can be used in the pFGAL4h/PJ69A reporter system. The yeast strain PJ69A contains besides
the GAL4 and GAL80 deletions also reporter genes coding for the yeast Ade2 and His3 proteins and for the
bacterial beta-galactosidase - all of them under the control of the Gal4-inducible Gal1 promoter. Thus
pFGAL4/PJ69A represents a specialised and sensitive system, which allows an enhancement of the measured signal
by in vivo coupled transcription and enzymatic detection. This system can be used for both measuring the ratio
of the beta-gal/luc enzymatic activities and searching for IRES activity by screening the colony growth rates
on selection media lacking adenine and histidine and containing various concentrations of the competitive
inhibitor of the histidine biosynthetic pathway.

Vector was created by IRESite curator - thus whole sequence is verified by above mentioned methods.

Citations:

Masek T., Vopalensky V., Horvath O., Vortelova L., Feketova Z., Pospisek M. (2007) Hepatitis C virus internal ribosome entry site initiates protein synthesis at the authentic initiation codon in yeast. J. Gen. Virol. 88(Pt 7):1992-2002

Last change to the database: 2019-03-18 09:32:49 GMT+1