IRESite record type: negative_control_plasmid_with_promoter_and_without_putative_IRES
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: both_UTRs_incomplete
The mRNA/+RNA description:
mRNA produced by control plasmid pFGAL4-L456 which comprises luciferase and Gal4 ORFs. L456 clone
was selected from pFGAL4::lambda library containing randomly cleaved phage lambda DNA for its comparable
length of intercistronic region to tested pFGAL4h-HCV1.
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: end-to-end_sequence_completely_same_as_in_the_experiment
L456 sequence was inserted into intercistronic position of pFGAL4 bicistronic plasmid to create bicistronic
control vector that can be used in the pFGAL4h/PJ69A reporter system. The yeast strain PJ69A contains besides
the GAL4 and GAL80 deletions also reporter genes coding for the yeast Ade2 and His3 proteins and for the
bacterial beta-galactosidase - all of them under the control of the Gal4-inducible Gal1 promoter. Thus
pFGAL4/PJ69A represents a specialised and sensitive system, which allows an enhancement of the measured signal
by in vivo coupled transcription and enzymatic detection. This system can be used for both measuring the ratio
of the beta-gal/luc enzymatic activities and searching for IRES activity by screening the colony growth rates
on selection media lacking adenine and histidine and containing various concentrations of the competitive
inhibitor of the histidine biosynthetic pathway.
Vector was created by IRESite curator - thus whole sequence is verified by above mentioned methods.