IRESite_Id:136 negative control plasmid pA

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `136`	Version: `1`
Originaly submitted by: Martin Mokrejš
Reviewed by: Martin Mokrejš	Last change: `2008-12-16 00:49:00`

IRESite record type:

negative_control_plasmid_with_promoter_and_without_putative_IRES

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
both_UTRs_incomplete

The mRNA/+RNA description:

Putative "monocistronic" mRNA derived from the empty plasmid with the STOP codon encoded in the intercistronic
region: GTCGACTTCCTCAG-TGA-TCCCCGAGGATC. Albeit huge the sequence provided here contains just the two CDS (the
second should be not functional) and the intercistronic region.

The mRNA/+RNA sequence represented in the +DNA notation:

atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg acggcagtta tctggaagat 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aatgtcgtcg acttcctcag TGAtcccgag 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

gatccttcaa cttccctgag ctcgaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag agcaactgca 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg gggaagcggt 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg taa
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771

Warning: available plasmid sequence is exactly same as the mRNA sequence. It seems we never managed to reconstruct the original plasmid sequence used to express the studied mRNA.

Credibility of mRNA sequence:

only_fragment_published_or_from_author_and_the_rest_is_a_guess

The name of the plasmid:
pA_LL The name of the promoter used to express this mRNA:
Lac

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg acggcagtta tctggaagat 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aatgtcgtcg acttcctcag TGAtcccgag 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

gatccttcaa cttccctgag ctcgaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag agcaactgca 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg gggaagcggt 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg taa
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

A promoter reported in cDNA corresponding to IRES sequence:
not tested The total number of notable open-reading frames (ORFs):
2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
LacZ

The description of the protein encoded in this ORF:
beta-galactosidase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1-3093

ORF

ORF position:

2

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
Luc

The description of the protein encoded in this ORF:
luciferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

3208-4773

Citations:

Maier D., Nagel A. C., Preiss A. (2002) Two isoforms of the Notch antagonist Hairless are produced by differential translation initiation. Proc. Natl. Acad. Sci. U. S. A. 99(24):15480-15485

Last change to the database: 2019-03-18 09:32:49 GMT+1