The 5'-UTR sequence was replaced by the 5'-UTR sequence used by Wang et al. (2005), which is by 33nt longer on
the 5'-end compared to the GenBank record. The 5'-UTR sequence used hereby was determined by 5'-RACE and thus
should be complete and is by 47nt longer than the sequence used by Johannes et al., 1999. The coding region
and 3'-UTR were taken over from GenBank.
Strong promoter activity was confirmed in human NIH3T3, Jurkat, HEK293, K562 cells. The promoter start sites
relative to ATG (+1) found in human cell lines are as follows: -412, -53, -33, -21. It is unclear whether the
extra 47 bases are necessary for the promoter activity observed by Wang et al. (2005) and not reported
by Johannes et al. (1999).
The IRES name: Pim-1 Warning: please make ires_name same as the gene_name and optionally append to it coordinates. E.g. when gene/virus name is EMCV-R use EMCV-R_-222_to_-1 or EMCV-R_1-456, etc. but not Emcv-R-... or EMCV-222_to_-1. Please keep case of letters as well. This rewards when searching through the database.
The IRES absolute position (the range includes START and STOP codons or their equivalents): 1-396
How IRES boundaries were determined: experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):
The formerly published Pim-1 IRES (Johannes et al., 1999) was found to contain promoter and in direct mRNA
transfection assays found not to have IRES activity (Wang et al., 2005, Figure 4C). The originally studied
Pim-1 5'-UTR in HeLa cells (Johannes et al., 1999) was shorter by 47nt compared to the 5'-RACE derived
sequence used by Wang et al. (2005) and was preceded by supposedly defective EMCV IRES (440 nt). The
"defective" EMCV possibly retained some activity, like ability to bind certain protein cofactors which could
have contributed the observed IRES activity of Pim-1.