IRESite record type: negative_control_plasmid_with_promoter_and_without_putative_IRES
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: 3UTR_incomplete
The mRNA/+RNA description:
Bicistronic in vivo transcript mRNA with chloramphenicol acetyltransferase and firefly luciferase genes with
fragment of the BVDV genome segment from polyprotein(nt 2198–2654) in the intercistronic region.
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: guessed_as_the_sequence_was_never_published_by_authors_nor_described_in_sufficient_detail
The description of the protein encoded in this ORF: firefly luciferase
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: no
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 1288-2940
This plasmid was used as the negative control for experiments in article Genetic Analysis of the Internal
Ribosome Entry Segment of Bovine Viral Diarrhea Virus (Chon et al., 1998; PMID:9837801).
This item is labeled as 3' UTR incomplete because the mRNA sequence deposited here ends at the stop codon of
the second cistron.