IRESite_Id:212 negative control plasmid pRSTF

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `212`	Version: `0`
Originaly submitted by: Martin Mokrejš
Reviewed by: Martin Mokrejš

IRESite record type:

negative_control_plasmid_with_promoter_and_without_putative_IRES

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
hopefully_full-length_mRNA

The mRNA/+RNA description:

In vitro T7 transcript containing renilla_luciferase - hairpin_from_multiple_cloning_site - firefly_luciferase
and terminated at XbaI site immediately after the STOP codon.

The mRNA/+RNA sequence represented in the +DNA notation:

GGctagccac catGacttcg aaagtttatg atccagaaca aaggaaacgg atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

tgattcattt attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac gcggcctctt cttatttatg gcgacatgtt 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

gtgccacata ttgagccagt agcgcggtgt attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg ttacttgatc 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ctatgagcat caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg gatgaatggc ctgatattga agaagatatt 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

gcgttgatca aatctgaaga aggagaaaaa atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag ttagaaccag 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

acctgacgtt gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg tttattgaat cggacccagg attcttttcc 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

aatgctattg ttgaaggtgc caagaagttt cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat gaaatgggaa 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaaatGtcg aTCGACgggg gcccctaggA gatccAAGCT TATCGATTTC GAACCCGGGG 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

TACCGAATTC CTCGAGTCTA GAGGAgcatg cTCCTCTAGA CTCGAGGAAT TCGGTACCCC GGGTTCGAAA TCGATAAGCT TgGATCTAGC GCTGGATCCA 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

TGGaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aatT
^2701      ^2711      ^2721      ^2731      ^2741      ^2751

Credibility of mRNA sequence:

end-to-end_sequence_reverse_engineered_and_should_match_experiment

The name of the plasmid:
pRSTF The name of the promoter used to express this mRNA:
T7

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

agatcgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc aggtaagtat 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtacTT AATACGACTC ACTATAGGct agccaccatG 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

acttcgaaag tttatgatcc agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac ttgatcatta caaatatctt 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

actgcatggt ttgaacttct taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag aaccagaaga atttgcagca 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa tgggaaaata tatcaaatcg 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

ttcgttgagc gagttctcaa aaatgaacaa atGtcgaTCG ACgggggccc ctaggAgatc cAAGCTTATC GATTTCGAAC CCGGGGTACC GAATTCCTCG 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

AGTCTAGAGG AgcatgcTCC TCTAGACTCG AGGAATTCGG TACCCCGGGT TCGAAATCGA TAAGCTTgGA TCTAGCGCTG GATCCATGGa agacgccaaa 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

gatatgggct cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

tcataaaggc caagaagggc ggaaagatcg ccgtgtaatT CTAGAgcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata agtttaaaca 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

gatccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

ccttttgctc acatggctcg ac
^5501      ^5511      ^5521

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

A promoter reported in cDNA corresponding to IRES sequence:
not tested The total number of notable open-reading frames (ORFs):
2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
RLuc

The description of the protein encoded in this ORF:
Renilla luciferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

12-968

ORF

ORF position:

2

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
FLuc

The description of the protein encoded in this ORF:
Firefly luciferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

1100-2752

Remarks:

A hairpin stemp loop is in the intercistronic region as is actually based on sequences from multiple cloning
site from pGEM-4z vector. Transcript is terminated at the XbaI site after the second cistron.

Citations:

Jang G. M., Leong L. E., Hoang L. T., Wang P. H., Gutman G. A., Semler B. L. (2004) Structurally distinct elements mediate internal ribosome entry within the 5'-noncoding region of a voltage-gated potassium channel mRNA. J. Biol. Chem. 279(46):47419-47430

Last change to the database: 2019-03-18 09:32:49 GMT+1