IRESite record type: negative_control_plasmid_with_promoter_and_without_putative_IRES
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: our_best_guess
The mRNA/+RNA description:
Putative spliced bicistronic mRNA derived from pCAT/LUC plasmid.
The mRNA/+RNA sequence represented in the +DNA notation:
Warning: mRNA sequence when devoid of trailing 'A's is still not a substring of the plasmid sequence. Is it because an intron is spliced out? Stay calm then. :-)
Credibility of mRNA sequence: guessed_as_the_sequence_was_never_published_by_authors_nor_described_in_sufficient_detail
The description of the protein encoded in this ORF: firefly luciferase
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: no
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 807-2459
Remarks:
This plasmid was used as the negative control for experiments in article A Secreted DNA-Binding Protein That
Is Translated through an Internal Ribosome Entry Site (IRES) and Distributed in a Discrete Pattern in the
Central Nervous System (Kim et al., 1998; PMID: 9790734).