Remarks:
Hypothetical mRNA sequence of NDST2 assembled in silico by two-step multiple sequence alignment polished by
manual editing. First, EST sequences matching the cloned 5'-UTR region obtained from IMAGE mouse Soares clone
1498585 (GI:3720009) (and re-deposited to GenBank under GI:21780280, 750bp) were aligned and resulting 5'-UTR
region consensus sequence partly overlapping to ORF region was created. The ORF overlapping sequence was used
to find NDST2 protein records, and their mRNA sequences were aligned to the consensus sequence created
previously. The final consensus sequence is this hypothetical mRNA and because the mRNAs used to create the
alignment did not have poly(A) tail so there was nothing to trim.
To get consensus sequence of 5'-UTR were used records GI# 22820019, 76234473, 76234563, 27176879, 27184368,
76273157, 26383535, 21780280 (the one reported by Grobe et al., 2002), 3720009, 26383556.
For consensus sequence of ORF and 3'-UTR were used records GI# 3720009, 113195687, 74211928, 83405502.
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