The name of the promoter used to express this mRNA: T7
Description of the plasmid (facultative for promoter-less plasmid records): pXL-Id is derivative of pXLJCon plasmid. It contains T7 and SP6 promoter sites and is typically used
in in vitro transcription/translation assays. Bicistronic RNA is transcribed by T7 polymerase after vector
linearization by EcoRI. IRES sequence (ranging from 502 to 1024 bp of Idefix sequence, GI: 4165192)
was inserted as EcoRI filled-in fragment into BamHI digested and blunted pXLJCon plasmid.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid: Id
The description of the protein encoded in this ORF: Truncated form of influenza NS1 protein which is N-terminally fused with 9 amino acids.
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: no
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 1927-2625
IRESite notes about verification of plasmid sequence:
Plasmid DNA was digested by EcoRI/SalI giving fragments 4202/1237 bp. Amplification with T7 and NS gene
specific primers (T7, ATACGACTCACTATAGGG,position within plasmid sequence:2785, NS_revseq,CTGCAACTCGTTTGCGGAC,
position within plasmid sequence:4801) gave one band of expected size. The complete sequence of IRES and 5-
and 3- flanking regions was determined by sequencing (region 3800-5172 of plasmid sequence). The rest of the
sequence presented here was reverse engineered with the help of authors description and IRESite entry 329.
There is one mutation detected in the vector backbone and 5 mutation in Idefix IRES in respect to
GI:4165192. More detailed data are annotated in the GenBank formatted sequence file provided by this page