The description of the protein encoded in this ORF: Firefly luciferase
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 1757-3409
The putative Apaf-1 IRES (read 5'-UTR) has been cloned in frame with the AUG codon of FLuc. Therefore, there
is missing 'ag' from the 3'-end of 5'-UTR to provide space for 'cc' bases for NcoI site (ccATGg).
Analysis of integrity of mRNA transcripts produced in vivo from this plasmid was done as a Northern-blot
analysis of poly(A)+ messages using randomly-primed probe against FLuc. Unexpected transcript of about 1.3kb
was detected in all lanes (pGL3, pGAL, pRF, pRAF).