Description of the plasmid (facultative for promoter-less plasmid records): Promoter-less variant of pEGFP-N1 (Clontech; #U55762) vector was prepared by excising the VspI/NheI fragment
containing the CMV IE promoter, filling-in the recessed 3'-termini by Klenow fragment of the DNA polymerase I
and by subsequent re-ligation of blunt ended vector.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:
GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
Plasmid sequence verified (partially/completely) by IRESite (more details in Remarks): plasmid sequence confirmed by IRESite curators by restriction analysis
The total number of notable open-reading frames (ORFs): 1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
The description of the protein encoded in this ORF: red-shifted variant of wild-type GFP optimized for brighter fluorescence and higher expression in mammalian
cells (Clontech)
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 97-816
Remarks:
The plasmid was used as the negative control for experiments in article Firefly luciferase gene contains a
cryptic promoter (Vopalensky et al., 2008).