Description of the plasmid (facultative for promoter-less plasmid records): L270 sequence was inserted into intercistronic position of pRG(-P) bicistronic plasmid (IRESiteID: 368) to
create tricistronic control vector.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:
GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
Plasmid sequence verified (partially/completely) by IRESite (more details in Remarks): plasmid sequence confirmed by IRESite curators by restriction analysis + parts by PCR + sequencing
The total number of notable open-reading frames (ORFs): 3
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
The description of the protein encoded in this ORF: red-shifted variant of wild-type GFP optimized for brighter fluorescence and higher expression in mammalian
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 1782-2501
The plasmid was used as the negative control for experiments in article Firefly luciferase gene contains a
cryptic promoter (Vopalensky et al., 2008; PMID:).
IRESite notes about verification of plasmid sequence:
The complete sequence of L270 region was determined by sequencing using DsRed1-C Sequencing Primer )
(Clontech; #6483-1; 5'-AGCTGGACATCACCTCCCACAACG-3').