Description of the plasmid (facultative for promoter-less plasmid records): Promoter-less variant of pRG-HCV1 vector (IRESite ID:) was prepared by excising the VspI/NheI fragment
containing the CMV IE promoter, filling-in the recessed 3'-termini by Klenow fragment of the DNA polymerase I
and by subsequent re-ligation of blunt ended vector.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested