The name of the promoter used to express this mRNA: T3
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing the luciferase coding region (luc version of the luciferase gene) between the promoter for
T3 RNA polymerase and the poly(A) tract.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:
GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
A promoter reported in cDNA corresponding to IRES sequence: not tested
The total number of notable open-reading frames (ORFs): 1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
The description of the protein encoded in this ORF: firefly luciferase
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 38-1690
Remarks:
The plasmid was used as the negative control for experiments in article Enhancement of IRES-Mediated
Translation of the c-myc and BiP mRNAs by the Poly(A) Tail Is Independent of Intact eIF4G and PABP (Thoma et
al., 2004; PMID: 15383282)