The name of the promoter used to express this mRNA: T3
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing 5' UTR of human c-myc transcribed from major P2 promoter (nt from -407 to -2) and firefly
luciferase coding region (luc version of the luciferase gene) with the poly(A)tract.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid: c-myc
The description of the protein encoded in this ORF: firefly luciferase
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 500-2155
IRESite notes about verification of plasmid sequence:
The complete sequence of IRES element was determined by sequencing using Fluc_seq_reverse
For detailed verification of whole plasmid sequence see item IRESite Id: 413.
The translation method used to study IRES function: in vitro
The in vitro translation system: HeLa cell lysate
The organism used for translation:
The temperature (in degrees of Celsia): 37
The relative translation efficiency in % of this IRES: 100.000
Name of the plasmid used as the negative control. pMiL-p(A)
IRESite Id of the plasmid used as negative control. 400
The relative translation efficiency in % of the negative control: 21.000
The effect of 5'-cap analogs on translation: no
Rapamycin affects translation: not tested
Type of RNA subject to translation: exogenous_RNA_with_ApppG_cap_with_polyA_tail
The translation of c-myc.IRES-pA RNA is about 3-fold more efficient than the translation of the
non-polyadenylated counterpart c-myc.IRES RNA (IRESite ID: 400).
Translational data are derived from Fig. 1A.