The nucleic acid data:
IRESite Id: 416 Version: 1
Originaly submitted by: Václav Vopálenský
Reviewed by: Martin Mokrejš Last change: 2008-06-26 16:01:57
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  our_best_guess
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  AEV
The genetic origin of this natural mRNA/+RNA:
  viral
The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
21389214
The mRNA/+RNA description: 
Avian encephalomyelitis virus, complete genome.
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  end-to-end_sequence_reverse_engineered_and_should_match_experiment
The organism containing this mRNA with IRES segment in its genome:
Avian encephalomyelitis virus Calnek
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
  1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 0
Originaly submitted by: Václav Vopálenský Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
polyprotein
The description of the protein encoded in this ORF:
Avian encephalomyelitis virus polyprotein.
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  no
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  495-6899
OPTIONAL: The function of the encoded protein
NP_653151.1 aev polyprotein [Avian encephalomyelitis virus]
NP_705595.1 putative VP4
NP_705596.1 putative VP2
NP_705597.1 putative VP3
NP_705598.1 putative VP1
NP_705599.1 H-rev107 homolog
NP_705600.1 membrane-associated protein
NP_705601.1 ATPase
NP_705602.1 membrane-associated protein
NP_705603.1 VPg
NP_705604.1 Chymotrypsin-like cysteine proteinase
NP_705605.1 RNA-dependent RNA polymerase
Citations:
Bakhshesh M., Groppelli E., Willcocks M. M., Royall E., Belsham G. J., Roberts L. O. (2008) The picornavirus avian encephalomyelitis virus possesses a hepatitis C virus-like internal ribosome entry site element. J. Virol. 82(4):1993-2003
IRESs:
IRES:
Version: 0
Originaly submitted by: Václav Vopálenský Reviewed by: Martin Mokrejš
The IRES name:
  AEV
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  1-494
Conclusion:
  strongly_supported_IRES
How IRES boundaries were determined:
experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
This IRES represents full-length 5'-UTR (1-494 bp) of AEV mRNA.

The AEV IRES element displays resistance to an inhibitor of eIF4A and functions well in the presence of
cleaved eIF4G.

Mutational analyses of AEV IRES shows similarities of AEV with HCV and PTV-1 IRES in domains IIIE and IIIf.

Deletion of 100 nt from the 5' end of the 5' UTR (mutant AEVm3) had some effect on IRES activity in cells,
reducing the activity to 50% of the activity of the wild type (wt).
Citations:
Bakhshesh M., Groppelli E., Willcocks M. M., Royall E., Belsham G. J., Roberts L. O. (2008) The picornavirus avian encephalomyelitis virus possesses a hepatitis C virus-like internal ribosome entry site element. J. Virol. 82(4):1993-2003
Last change to the database: 2015-04-16 16:45:23 GMT+1