IRESite record type: plasmid_with_promoter_and_putative_IRES_without_translational_characterization
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: hopefully_full-length_mRNA
The mRNA/+RNA description:
In vitro T7 run-off transcript containing full (except very 5'-most Adenosine) gypsy 5' UTR (nt from -841 to
+5 of the original sequence) mRNA cloned upstream of the truncated version of the beta-galactosidase
(linearized the AflIII site at position 3074 within pMC-WT; see 1393.gb below).
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: end-to-end_sequence_reverse_engineered_and_should_match_experiment
The name of the promoter used to express this mRNA: T7
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing full (except very 5'-most Adenosine) gypsy 5' UTR (nt from -841 to +5 of the original
sequence) and beta-galactosidase coding region.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid: gypsy
The IRES absolute position (the range includes START and STOP codons or their equivalents): 43-888
How IRES boundaries were determined: experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):
Gypsy IRES represents the full-length (except very 5'-most Adenosine) gypsy 5' UTR (841 nucleotides) plus
first 5 nucleotides from the viral polyprotein.
Translation of this monocistronic RNA in the RRL revealed that capping of the transcript did not increase
protein synthesis; the presence of a cap structure at the 5' end of the mRNA did not enhance expression driven
by the gypsy 5' UTR in the RRL system (see Fig. 2A).