A promoter reported in cDNA corresponding to IRES sequence: no
The total number of notable open-reading frames (ORFs): 1
Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:
Summary of experiments studying integrity of the in vivo transcripts in a particular host:
Integrity (uniformity) of mRNA tested using Northern-blot: homogeneous_population_of_molecules_confirmed
Integrity (uniformity) of mRNA tested using RNase protection: not_tested
Integrity (uniformity) of mRNA tested using 5'-RACE: not_tested
Integrity (uniformity) of mRNA tested using primer extension : not_tested
Integrity (uniformity) of mRNA tested using RT-PCR: not_tested
Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR): not_tested
Integrity (uniformity) of mRNA tested using RNAi: not_tested
Integrity (uniformity) of mRNA tested using S1 nuclease mapping: not_tested
Cryptic promoter presence was confirmed by expression from a promoter-less plasmid: no_promoter_confirmed
Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter: not_tested
Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks: not_tested
The abbreviated name of this ORF/gene: p58_PITSLRE
The description of the protein encoded in this ORF: PITSLRE alpha 2-2
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 1126-2445
Remarks:
The p58 protein is produced by IRES-mediated translation initiation whereas p110 is initiated by cap-dependent
manner. Survey of the ATG codons within 5'-UTR was shown in Cornelis et al. (2000), Table 1.
Promoter-less plasmid was used to show at least 20x less-active promoter could be located in 745-1125 PITSLRE
IRES as compared to SV40 early promoter (Tinton et al. (2005), Figure 1D).
The IRES absolute position (the range includes START and STOP codons or their equivalents): 745-1125
Conclusion: putative_IRES
How IRES boundaries were determined: experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):
Remarks:
Sequence 6 from patent US 6764852. Originally IRES has been mapped to 121-1125 and with finer mapping
published in Tinton et al. (2005) to 745-1125 in Di-4 plasmid and 976-1125 (Di-4 mut C).