IRESite record type: negative_control_plasmid_with_promoter_and_without_putative_IRES
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: hopefully_full-length_mRNA
The mRNA/+RNA description:
Putative spliced in vivo SV40 promoter-derived transcript produced from bicistronic plasmid pstemRL which
comprises renilla and firefly luciferases as the first and the second cistron, respectively with a hairpin in
front of the first cistron.
The sequence ends at its 3'-end right after the poly(A) signal from SV40 mRNA and thus the 3'-UTR might be
The mRNA/+RNA sequence represented in the +DNA notation:
Warning: mRNA sequence when devoid of trailing 'A's is still not a substring of the plasmid sequence. Is it because an intron is spliced out? Stay calm then. :-)
Credibility of mRNA sequence: end-to-end_sequence_completely_same_as_in_the_experiment
The name of the promoter used to express this mRNA: SV40
Description of the plasmid (facultative for promoter-less plasmid records): Plasmid containing renilla and firefly luciferase reporter genes. A synthetic DNA fragment was inserted into
the bicistronic constructs to introduce a stable stem loop structure (deltaG = - 55 Kcal/mol) 169 nt
downstream from transcription start site.
Plasmid pstemRF was constructed by excising the c-myc 5' UTR from pstemRmycF (IRESite ID: 355) by digesting
with SpeI/NcoI, blunt-ending with T4 DNA polymerase and ligating shut.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:
GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
A promoter reported in cDNA corresponding to IRES sequence: not tested
The total number of notable open-reading frames (ORFs): 2
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence: