The nucleic acid data:
IRESite Id: 46 Version: 6
Originaly submitted by: Martin Pospíšek Submission date: 2005-07-19 00:00:00
Reviewed by: Václav Vopálenský Last change: 2009-09-01 16:39:00
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  our_best_guess
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  Hsp101
The genetic origin of this natural mRNA/+RNA:
  nuclear
The GenBankId GI:# number of the most similar mRNA/+RNA sequence to this one.
4928487 
The mRNA/+RNA description: 
Zea mays heat shock protein 101 (HSP101) gene. Poly(A) sequence was dropped.
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  only_fragment_published_or_from_author
The organism containing this mRNA with IRES segment in its genome:
Zea mays
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
  1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 2 Last change: 2009-09-01 16:37:24
Originaly submitted by: Martin Pospíšek Reviewed by: Václav Vopálenský
The abbreviated name of this ORF/gene:
Hsp101
The description of the protein encoded in this ORF:
heat shock protein 101
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  no
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  145-2883
Remarks:
The underlying mRNA sequence used for this record has start codon at base 145 instead of 147. It also lacks
the region of 60nt upstream the transcription start which has been included in the bicistronic tests. See
GI:68160574 for sequence of the relevant Zea mays chromosome region. Also, a full-length cDNA under
GI:223975968 has only 68bp as the 5'-UTR. A third mRNA sequence available has 114bp as the 5'-UTR
(GI:40060484).
Please refer also to sequence 39 from Patent WO03020928.
Citations:
Dinkova T. D., Zepeda H., Martinez-Salas E., Martinez L. M., Nieto-Sotelo J., de Jimenez E. S. (2005) Cap-independent translation of maize Hsp101. Plant J. 41(5):722-731
IRESs:
IRES:
Version: 9 Last change: 2007-01-12 00:00:00
Originaly submitted by: Martin Pospíšek Reviewed by: Václav Vopálenský
The IRES name:
  Hsp101
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  1-161
Conclusion:
  putative_IRES
How IRES boundaries were determined:
guessed
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
In described experiment is Hsp101 5' UTR cloned in bicistronic plasmids contained chloramphenicol acetyl
transferase as first cistron and firefly luciferase as second cistron. The maize "Hsp101 5' UTR" used in the
bicistronic constructs included 60 nt upstream of the transcription start site, the entire 5' UTR (146 bp),
and the first 15 bp of the coding region in frame with the LUC ORF. Here is shown mRNA (UTR (146 nt) and open
reading frame (15 nt)) only. Therefore, T3/T7 capped transcripts used for in vitro translations contained in
roughly the first 60nt of a plant promoter. In vitro translation was let to proceed for 1 hour at 30
oC. FMDV Lb protease was supplemented to rabbit reticulocyte lysates used for translation of the bicistronic
mRNAs. Protein products were detected by antibodies. Alternatively, wheat germ extracts (and also rabbit
reticulocyte lysates) were depleted of eIF4E because plant eIF4G proteins are not susceptible to FMDV Lb
protease.
Citations:
Dinkova T. D., Zepeda H., Martinez-Salas E., Martinez L. M., Nieto-Sotelo J., de Jimenez E. S. (2005) Cap-independent translation of maize Hsp101. Plant J. 41(5):722-731
Last change to the database: 2015-04-16 16:45:23 GMT+1