IRESite_Id:472 promoter-less plasmid (deltaCMV)betaGAL/5'(-162)/CAT used to evaluate potential promoter activity in XIAP IRES

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `472`	Version: `0`
Originaly submitted by: Václav Vopálenský
Reviewed by: Martin Mokrejš

IRESite record type:

promoter-less_plasmid_with_putative_IRES

The name of the plasmid:
(deltaCMV)betaGAL/5'(-162)/CAT

Description of the plasmid (facultative for promoter-less plasmid records):
Part of the 5' UTR from human XIAP mRNA (from nt -165 to nt -1 of the original sequence) has been cloned between the beta galactosidase and chloramphenicol acetyl transferase reporter genes. This promoter-less variant of pbetaGAL/5'(-162)/CAT (IRESite ID: 486) was prepared by removing the CMV promoter using NruI and HindIII restriction sites.

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

A promoter reported in cDNA corresponding to IRES sequence:

not tested

The origin of IRES in the plasmid:

nuclear

The donor organism of the IRES segment:

Homo sapiens

The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
XIAP

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gaatctgctt agggttaggc gttttgcgct gcttcgagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc tgcagatatc 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

catcacactg gcggccgcaa ttcccgggga tcgaaagagc ctgctaaagc aaaaaagaag tcaccatgtc gtttactttg accaacaaga acgtgatttt 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

cgttgccggt ctgggaggca ttggtctgga caccagcaag gagctgctca agcgcgatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

gctgcgttgg agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattaca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

caaaaataaT AATAACCGGG CAGGCCATGT CTGCCCGTAT TTCGCGTAAG GAAATCCATT ATGTACTATT TAAAAAACAC AAACTTTTGG ATGTTCGGTT 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

TATTCTTTTT CTTTTACTTT TTTATCATGG GAGCCTACTT CCCGTTTTTC CCGATTTGGC TACATGACAT CAACCATATC AGCAAAAGTG ATACGGGTAT 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

TATTTTTGCC GCTATTTCTC TGTTCTCGCT ATTATTCCAA CCGCTGTTTG GTCTGCTTTC TGACAAACTC GGCCTCGACT CTAGGCGGCC GCTCGAGAAT 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

TAGAATGTTT CTTAGCGGTC GTGTAGTTAT TTTTATGTCA TAAGTGGATA ATTTGTTAGC TCCTATAACA AAAGTCTGTT GCTTGTGTTT CACATTTTGG 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

ATTTCCTAAT ATAATGTTCT CTTTTTAGAA AAGGTGGACA AGTCCTATTT TCAAGAGAAG CTCGAGCTAG AGGATCTGAG CTTGGCGAGA TTTTCAGGAG 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

CTAAGGAAGC TAAAatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg ccggatcttt gtgaaggaac cttacttctg tggtgtgaca 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

atcatgtctg cctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
^8001      ^8011      ^8021      ^8031      ^8041      ^8051      ^8061      ^8071      ^8081      ^8091      

gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
^8101      ^8111      ^8121      ^8131      ^8141      ^8151      ^8161      ^8171      ^8181      ^8191      

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
^8201      ^8211      ^8221      ^8231      ^8241      ^8251      ^8261      ^8271      ^8281      ^8291      

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
^8301      ^8311      ^8321      ^8331      ^8341      ^8351      ^8361      ^8371      ^8381      ^8391      

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
^8401      ^8411      ^8421      ^8431      ^8441      ^8451      ^8461      ^8471      ^8481      ^8491      

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
^8501      ^8511      ^8521      ^8531      ^8541      ^8551      ^8561      ^8571      ^8581      ^8591      

tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
^8601      ^8611      ^8621      ^8631      ^8641      ^8651      ^8661      ^8671      ^8681      ^8691      

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
^8701      ^8711      ^8721      ^8731      ^8741      ^8751      ^8761      ^8771      ^8781      ^8791      

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
^8801      ^8811      ^8821      ^8831      ^8841      ^8851      ^8861      ^8871      ^8881      ^8891      

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
^8901      ^8911      ^8921      ^8931      ^8941      ^8951      ^8961      ^8971      ^8981      ^8991      

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
^9001      ^9011      ^9021      ^9031      ^9041      ^9051      ^9061      ^9071      ^9081      ^9091      

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
^9101      ^9111      ^9121      ^9131      ^9141      ^9151      ^9161      ^9171      ^9181      ^9191      

cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
^9201      ^9211      ^9221      ^9231      ^9241      ^9251      ^9261      ^9271      ^9281      ^9291      

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
^9301      ^9311      ^9321      ^9331      ^9341      ^9351      ^9361      ^9371      ^9381      ^9391      

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
^9401      ^9411      ^9421      ^9431      ^9441      ^9451      ^9461      ^9471      ^9481      ^9491      

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
^9501      ^9511      ^9521      ^9531      ^9541      ^9551      ^9561      ^9571      ^9581      ^9591      

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
^9601      ^9611      ^9621      ^9631      ^9641      ^9651      ^9661      ^9671      ^9681      ^9691      

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
^9701      ^9711      ^9721      ^9731      ^9741      ^9751      ^9761      ^9771      ^9781      ^9791      

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
^9801      ^9811      ^9821      ^9831      ^9841      ^9851      ^9861      ^9871      ^9881      ^9891      

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
^9901      ^9911      ^9921      ^9931      ^9941      ^9951      ^9961      ^9971      ^9981      ^9991      

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcc
^10001     ^10011     ^10021     ^10031     ^10041

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

The total number of notable open-reading frames (ORFs):

2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
LacZ

The description of the protein encoded in this ORF:
beta galactosidase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

466-3609

ORF

ORF position:

2

Version: `1`	Last change: `2009-09-03 20:03:16`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
CAT

The description of the protein encoded in this ORF:
chloramphenicol acetyl transferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

4115-4774

Remarks:

The plasmid was used as the negative control for experiments in article A search for structurally similar
cellular internal ribosome entry sites (Baird et al., 2007; PMID:17591613).

Citations:

Baird S. D., Lewis S. M., Turcotte M., Holcik M. (2007) A search for structurally similar cellular internal ribosome entry sites. Nucleic. Acids. Res. 35(14):4664-4677

IRESs:

IRES:

Version: `1`	Last change: `2009-09-03 20:03:16`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš
The IRES name: `XIAP`	The functional status of IRES: `functional`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

3896-4060

How IRES boundaries were determined:
experimentally_determined

5'-end of IRES relative to last base of the STOP codon of the upstream ORF: `287`	3'-end of IRES relative to last base of the STOP codon of the upstream ORF: `451`
5'-end of IRES relative to first base of the START codon of the downstream ORF: `-219`	3'-end of IRES relative to first base of the START codon of the downstream ORF: `-55`

The sequence of IRES region aligned to its secondary structure (if available):

AGAATTAGAA TGTTTCTTAG CGGTCGTGTA GTTATTTTTA TGTCATAAGT GGATAATTTG TTAGCTCCTA TAACAAAAGT CTGTTGCTTG TGTTTCACAT 
^3896      ^3906      ^3916      ^3926      ^3936      ^3946      ^3956      ^3966      ^3976      ^3986      

TTTGGATTTC CTAATATAAT GTTCTCTTTT TAGAAAAGGT GGACAAGTCC TATTTTCAAG AGAAG
^3996      ^4006      ^4016      ^4026      ^4036      ^4046      ^4056

Remarks:

XIAP IRES represents part of XIAP 5' UTR (nt from -165 to nt -1 of the original sequence). This region
retained full IRES activity comparable with the whole 5' UTR (from nt -993 to nt -1) of XIAP mRNA.

Citations:

Baird S. D., Lewis S. M., Turcotte M., Holcik M. (2007) A search for structurally similar cellular internal ribosome entry sites. Nucleic. Acids. Res. 35(14):4664-4677

Last change to the database: 2019-03-18 09:32:49 GMT+1