Description of the plasmid (facultative for promoter-less plasmid records): Part of the 5' UTR from human XIAP mRNA (from nt -165 to nt -1 of the original sequence) has been cloned
between the beta galactosidase and chloramphenicol acetyl transferase reporter genes.
This promoter-less variant of pbetaGAL/5'(-162)/CAT (IRESite ID: 486) was prepared by removing the CMV
promoter using NruI and HindIII restriction sites.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested
The IRES absolute position (the range includes START and STOP codons or their equivalents): 3896-4060
How IRES boundaries were determined: experimentally_determined
5'-end of IRES relative to last base of the STOP codon of the upstream ORF: 287
3'-end of IRES relative to last base of the STOP codon of the upstream ORF: 451
5'-end of IRES relative to first base of the START codon of the downstream ORF: -219
3'-end of IRES relative to first base of the START codon of the downstream ORF: -55
The sequence of IRES region aligned to its secondary structure (if available):
XIAP IRES represents part of XIAP 5' UTR (nt from -165 to nt -1 of the original sequence). This region
retained full IRES activity comparable with the whole 5' UTR (from nt -993 to nt -1) of XIAP mRNA.