Description of the plasmid (facultative for promoter-less plasmid records): Almost the full length 5' UTR from human AQP4 mRNA (from nt -275 to nt -1 of the original sequence) and the
first 7 nts (+1 to +7) from AQP4 ORF has been cloned between the beta galactosidase and chloramphenicol acetyl
transferase reporter genes.
This promoter-less variant of pBiCAQP4 (IRESite ID: 488) was prepared by removing the CMV promoter using NruI
and HindIII restriction sites.
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?): not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing: not tested
A promoter reported in cDNA corresponding to IRES sequence: not tested