Putative spliced in vivo transcript containing synthetic KMI1 IRES.
The synthetic IRES was also cloned into pBRRF promoter-less vector and no cryptic promoter was found in that
setup (Figure 1F).
Synthetic IRES sequence from Figure 1 forming hairpin loop structure crucial for its activity.
Data from Figure 1C. In addition, in the Figure 1E is show that RNAi knock-down of PTB-1 to 10% of the normal
level drops KMI2 IRES activity to 38%.