The nucleic acid data:
IRESite Id: 49 Version: 5
Originaly submitted by: Václav Vopálenský Submission date: 2005-07-20 00:00:00
Reviewed by: Václav Vopálenský Last change: 2008-07-12 17:53:30
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  possibly_wrong
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  MYT2
The genetic origin of this natural mRNA/+RNA:
  nuclear
The GenBankId GI:# number of the most similar mRNA/+RNA sequence to this one.
2246660 
The mRNA/+RNA description: 
Homo sapiens myelin transcription factor 2 (MYT2) mRNA
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  guessed_as_the_sequence_was_never_published_by_authors_nor_described_in_sufficient_detail
The organism containing this mRNA with IRES segment in its genome:
Homo sapiens
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
  1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 0
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The abbreviated name of this ORF/gene:
MYT2
The description of the protein encoded in this ORF:
Myelin transcription factor 2
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  no
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  1156-2283
OPTIONAL: The function of the encoded protein
GO:0005615;  Cellular component: extracellular space
GO:0005634;  Cellular component: nucleus
GO:0003697;  Molecular function: single-stranded DNA binding
GO:0003700;  Molecular function: transcription factor activity
GO:0008270;  Molecular function: zinc ion binding
GO:0008283;  Biological process: cell proliferation
Remarks:
MYT2 mRNA can only be translated efficiently in vitro in rabbit reticulocyte lysates only when upstream ATGs
(and possibly secondary structures) are removed. This mRNA is said to be low-abundant and undetectable using
Northern blot or RNase-protection assay in total cellular RNA. It seems the MYT2 gene contains an intron. The
IRES activity was studied only in vivo using bicistronic constructs. The underlying sequence probably differs
from the clone used by the authors. RT-PCR analysis of mouse mRNA has shown 2 types of MYT2 mRNA. One
full-length as expected and one shorter by 40 bases in 5'-UTR (missing bases 948-987, but not resembling
splice junction).
Citations:
Kim J. G., Armstrong R. C., Berndt J. A., Kim N. W., Hudson L. D. (1998) A secreted DNA-binding protein that is translated through an internal ribosome entry site (IRES) and distributed in a discrete pattern in the central nervous system. Mol. Cell. Neurosci. 12(3):119-140
IRESs:
IRES:
Version: 6 Last change: 2008-07-12 17:53:30
Originaly submitted by: Václav Vopálenský Reviewed by: Václav Vopálenský
The IRES name:
  MYT2_997-1152
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  997-1152
Conclusion:
  putative_IRES
How IRES boundaries were determined:
guessed
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
Fragment of 5' UTR nt 997 to nt 1152 (determined by primer location) is 156-nt IRES module, which is
functional as IRES when it is tested in bicistronic vector. The sequence of the IRES regions differs from the
sequence shown in Fig. 1 which claims complete 5' UTR was tested [shown underlined].
Citations:
Kim J. G., Armstrong R. C., Berndt J. A., Kim N. W., Hudson L. D. (1998) A secreted DNA-binding protein that is translated through an internal ribosome entry site (IRES) and distributed in a discrete pattern in the central nervous system. Mol. Cell. Neurosci. 12(3):119-140
Last change to the database: 2015-04-16 16:45:23 GMT+1