The nucleic acid data:
IRESite Id: 543 Version: 1
Originaly submitted by: Martin Mokrejš Submission date: 2008-12-19 17:36:45
Reviewed by: Martin Mokrejš Last change: 2009-04-13 18:16:18
IRESite record type:
  plasmid_with_promoter_and_putative_IRES_without_translational_characterization
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  hopefully_full-length_mRNA
The mRNA/+RNA description: 
Putative spliced in vivo CMV IE promoter-derived transcript up to the end of the SV40 late poly(A) signal.
The mRNA/+RNA sequence represented in the +DNA notation:


Warning: mRNA sequence when devoid of trailing 'A's is still not a substring of the plasmid sequence. Is it because an intron is spliced out? Stay calm then. :-)
Credibility of mRNA sequence:
  reverse_engineered_sequence_and_should_match_experiment_maybe_except_3UTR
The name of the plasmid:
pIRES
The name of the promoter used to express this mRNA:
  CMV_IE
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):
  not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing:
  not tested
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
EMCV-R
The origin of IRES in the plasmid:
  viral
The donor organism of the IRES segment:
Encephalomyocarditis virus Rueckert
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:


GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
pIRES.jpg
The total number of notable open-reading frames (ORFs):
  1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
fragment
The description of the protein encoded in this ORF:
fragment of the viral polyprotein
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  828-860
Remarks:
The empty vector as provided by Promega/Clontech/BD Biosciences lacking reporter proteins immediately
preceding the EMCV IRES and immediately behind it. There is a remnant of the viral polyprotein CDS so we
annotated that small ORF along this record although it is not much meaningful as virtually nobody uses the empty
vector for "protein expression".

Original vendor information: http://www.clontech.com/products/detail.asp?tabno=2&product_id=10480
IRESs:
IRES:
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The IRES name:
  EMCV-R+12_mut
The functional status of IRES:
  functional
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  261-847
How IRES boundaries were determined:
experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
The EMCV-R IRES with first 12 nucleotides of the viral polyprotein. Please note this is the variant of EMCV
IRES highly PTB protein (ITAF) dependent. For more details refer to the summary record on EMCV IRES in
IRESite.
Last change to the database: 2015-04-16 16:45:23 GMT+1