The nucleic acid data:
IRESite Id: 544 Version: 1
Originaly submitted by: Martin Mokrejš Submission date: 2008-12-20 16:26:53
Reviewed by: Martin Mokrejš Last change: 2011-04-08 22:11:58
IRESite record type:
  plasmid_with_promoter_and_putative_IRES_without_translational_characterization
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  3UTR_incomplete
The mRNA/+RNA description: 
Putative in vitro T7 promoter derived transcript of the empty vector up to the linearization PstI site.
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  reverse_engineered_sequence_and_should_match_experiment_except_3UTR
The name of the plasmid:
pCITE-2a
The name of the promoter used to express this mRNA:
  T7
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):
  not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing:
  not tested
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
EMCV-R
The origin of IRES in the plasmid:
  viral
The donor organism of the IRES segment:
Encephalomyocarditis virus Rueckert
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:


GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
pCITE-2a.jpg
The total number of notable open-reading frames (ORFs):
  1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
fusion_protein
The description of the protein encoded in this ORF:
fusion protein spanning the MCS site and His tag
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  519-626
Remarks:
This is the ORF encoded by the empty vector and annotated here for completeness. However, it is always
destroyed by insertion of a reporter protein into the multiple cloning site of the vector. The initiator ATG
codon is overlapped by NcoI site.

Original information from Novagen: http://www.emdbiosciences.com/Products/ProductDisplay.asp?catno=69290
IRESs:
IRES:
Version: 1 Last change: 2011-04-08 22:11:58
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The IRES name:
  EMCV-R_335-834
The functional status of IRES:
  functional
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  17-516
How IRES boundaries were determined:
experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):


Last change to the database: 2015-04-16 16:45:23 GMT+1