IRESite record type: negative_control_plasmid_with_promoter_and_without_putative_IRES
The shape of the nucleic acid molecule translated: linear
The quality of the mRNA/+RNA sequence: our_best_guess
The mRNA/+RNA description:
Putative in vivo transcript driven from CMV IE promoter and terminated after the SV40 late poly(A) signal
containing GLuc (Gaussia luciferase) and GFP cistrons separated by presumed non-IRES containing sequence from
gamma-actin 5'-UTR.
The mRNA/+RNA sequence represented in the +DNA notation:
Credibility of mRNA sequence: end-to-end_sequence_reverse_engineered_and_should_match_experiment
The description of the protein encoded in this ORF: green fluorescent protein
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 738-1457
Remarks:
The non-IRES sequence is the complete 5'-UTR of gamma-actin (-74 to -1). It is located in the mRNA in
positions 664-737. The "activity" was about 25.7% of EMCV in HeLa cells (23.2% in NIH3T3 cells) and in
general, close to BiP, eIF4G, NRF, Rbm3, see Figures 1 B and C.