The nucleic acid data:
IRESite Id: 580 Version: 1
Originaly submitted by: Martin Mokrejš Submission date: 2009-04-09 02:50:48
Reviewed by: Martin Mokrejš Last change: 2009-07-16 15:27:48
IRESite record type:
  plasmid_with_promoter_and_putative_IRES_translationally_characterized
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  only_IRES_fragment
The mRNA/+RNA description: 
FeLV-Notch2 IRES fragment 580-814.
The mRNA/+RNA sequence represented in the +DNA notation:


Warning: available plasmid sequence is exactly same as the mRNA sequence. It seems we never managed to reconstruct the original plasmid sequence used to express the studied mRNA.
Credibility of mRNA sequence:
  only_fragment_published_or_from_author
The name of the plasmid:
pSV-CAT/FeLV-Notch2-580_814/LUC
The name of the promoter used to express this mRNA:
  SV40
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):
  not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing:
  not tested
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
FeLV-Notch2
The origin of IRES in the plasmid:
  viral
The donor organism of the IRES segment:
Felis silvestris 9683
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:


GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
pSV-CAT/FeLV-Notch2-580_814/LUC.jpg
The total number of notable open-reading frames (ORFs):
  0
Remarks:
Please note we do not have sequence of the vector not even of the ORFs flanking the putative IRES.
Citations:
Lauring A. S., Overbaugh J. (2000) Evidence that an IRES within the Notch2 coding region can direct expression of a nuclear form of the protein. Mol. Cell. 6(4):939-945
IRESs:
IRES:
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The IRES name:
  FeLV-Notch2_580-814
The functional status of IRES:
  functional
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  1-233
How IRES boundaries were determined:
experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):


Citations:
Lauring A. S., Overbaugh J. (2000) Evidence that an IRES within the Notch2 coding region can direct expression of a nuclear form of the protein. Mol. Cell. 6(4):939-945
The translation experiments:
Translation results:
IRESite Translation Id: 617
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The translation method used to study IRES function:
in vivo
The organism used for translation:
Homo sapiens HeLa (ATCC CCL-2)
The temperature (in degrees of Celsia):
37
The relative translation efficiency in % of this IRES:
  288.000
Name of IRES used as the positive control:
  PV
Name of the plasmid used as the positive control.
pSV-CAT/polio/LUC
Name of the plasmid used as the negative control.
pSV-CAT/mutEMCV/LUC
The relative translation efficiency in % of the positive control:
  100.000
The relative translation efficiency in % of the negative control:
  13.000
The effect of 5'-cap analogs on translation:
not tested
Rapamycin affects translation:
not tested
Type of RNA subject to translation:
  endogenous_nuclear_RNA_Pol_II_transcript
Remarks:
Figure 3B.
Citations:
Lauring A. S., Overbaugh J. (2000) Evidence that an IRES within the Notch2 coding region can direct expression of a nuclear form of the protein. Mol. Cell. 6(4):939-945
Last change to the database: 2015-04-16 16:45:23 GMT+1