The nucleic acid data:
IRESite Id: 589 Version: 0
Originaly submitted by: Martin Mokrejš Submission date: 2009-04-14 17:45:58
Reviewed by: Martin Mokrejš
IRESite record type:
The shape of the nucleic acid molecule translated:
The quality of the mRNA/+RNA sequence:
The mRNA/+RNA description: 
In vivo spliced monocistronic CMV IE promoter driven transcript containing firefly luciferase terminated after
the SV40 poly(A) signal.
The mRNA/+RNA sequence represented in the +DNA notation:

Warning: mRNA sequence when devoid of trailing 'A's is still not a substring of the plasmid sequence. Is it because an intron is spliced out? Stay calm then. :-)
Credibility of mRNA sequence:
The name of the plasmid:
The name of the promoter used to express this mRNA:
The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):
  not tested
The in vivo produced heterogeneous transcripts occur due to alternative splicing:
  not tested
The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF position:   1
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
The description of the protein encoded in this ORF:
Firefly luciferase
The translational frameshift (ribosome slippage) involved:
The ribosome read-through involved:
The alternative forms of this protein occur by the alternative initiation of translation:
  not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
Petz M., Kozina D., Huber H., Siwiec T., Seipelt J., Sommergruber W., Mikulits W. (2007) The leader region of Laminin B1 mRNA confers cap-independent translation. Nucleic Acids Res. 35(8):2473-2482
Last change to the database: 2019-03-18 09:32:49 GMT+1