The relative translation efficiency in % of this IRES: 829.000
Name of IRES used as the positive control: EMCV-R
Name of the plasmid used as the positive control. pEMCV-F
Name of the plasmid used as the negative control. pF
IRESite Id of the plasmid used as positive control. 590
IRESite Id of the plasmid used as negative control. 589
The relative translation efficiency in % of the positive control: 100.000
The relative translation efficiency in % of the negative control: 120.000
The effect of 5'-cap analogs on translation: not tested
Rapamycin affects translation: not tested
Type of RNA subject to translation: endogenous_nuclear_RNA_Pol_II_transcript
Data from Figure 3A. The values were normalized to beta-galactosidase (co-transfection control).
Additional notes from M Petz:
Concerning Fig. 3A, I have checked the data again. The experiment was repeated several times and the ß-Gal
activities of the cotransfected plamid were always quite equal. The normalisation to the ß-gal values did
not affect the ratio of the firefly activities in any significant way.