The nucleic acid data:
IRESite Id: 602 Version: 1
Originaly submitted by: Martin Mokrejš Submission date: 2009-08-21 19:36:02
Reviewed by: Martin Mokrejš Last change: 2009-08-21 21:07:01
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  our_best_guess
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  crTMV
The genetic origin of this natural mRNA/+RNA:
  viral
The GenBankId GI:# number of the most similar mRNA/+RNA sequence to this one.
488713 
The mRNA/+RNA description: 
Bicistronic, uncapped subgenomic I2 RNA (sgRNA) of crTMV. Only the movement protein (MP) is produced while
coat protein (CP) is not.
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  only_fragment_published_or_from_author_and_the_rest_is_a_guess
The organism containing this mRNA with IRES segment in its genome:
Crucifer tobamovirus
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
  1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
MP
The description of the protein encoded in this ORF:
movement protein
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  76-879
Remarks:
The 5'-UTR region was inferred from TRSIG database record 'EXPERIMENT E0074'
(http://dna01.bse.kyutech.ac.jp/jouhou/trsig/anotates.html?-page+LibInfo+-lib+TRSIG_OB).
Citations:
Ivanov P. A., Karpova O. V., Skulachev M. V., Tomashevskaya O. L., Rodionova N. P., Dorokhov YuL, Atabekov J. G. (1997) A tobamovirus genome that contains an internal ribosome entry site functional in vitro. Virology. 232(1):32-43
Dorokhov YuL, Ivanov PA, Novikov VK, Agranovsky AA, Morozov SYu, Efimov VA, Casper R, Atabekov JG (1994) Complete nucleotide sequence and genome organization of a tobamovirus infecting cruciferae plants. FEBS Lett. 1(350):5-8
IRESs:
IRES:
Version: 1 Last change: 2009-08-25 10:41:07
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The IRES name:
  crTMV_IREScp
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  655-802
Conclusion:
  putative_IRES
How IRES boundaries were determined:
experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
The IRES is not functional in this context (I2 sgRNA) although it was reported functional in in vitro assays.
More details in IRESiteId:36.
Citations:
Ivanov P. A., Karpova O. V., Skulachev M. V., Tomashevskaya O. L., Rodionova N. P., Dorokhov YuL, Atabekov J. G. (1997) A tobamovirus genome that contains an internal ribosome entry site functional in vitro. Virology. 232(1):32-43
Last change to the database: 2015-04-16 16:45:23 GMT+1