The nucleic acid data:
IRESite Id: 616 Version: 0
Originaly submitted by: Martin Mokrejš Submission date: 2009-08-29 17:15:07
Reviewed by: Martin Mokrejš
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  hopefully_full-length_mRNA
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  HiPV
The genetic origin of this natural mRNA/+RNA:
  viral
The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
3493357
The mRNA/+RNA description: 
Himetobi P virus genomic RNA, complete sequence.
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  end-to-end_sequence_reverse_engineered_and_should_match_experiment
The organism containing this mRNA with IRES segment in its genome:
Himetobi P virus
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
  2
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
NS
The description of the protein encoded in this ORF:
non-structural protein precursor
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  964-6297
ORF
ORF position:   2
Version: 0
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
CP
The description of the protein encoded in this ORF:
capsid protein precursor (36.5 kDa)
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  6473-9097
Remarks:
The virus infects Laodelphax striatellus. It is presumed the capsid protein precursor is initiated at a CUA
codon and that the IRES-region contains several loop structures forming pseudoknots. In PSIV the capsid
protein precursor is initiated from a CUU codon.
Citations:
Nakashima N, Sasaki J, Toriyama S (1999) Determining the nucleotide sequence and capsid-coding region of himetobi P virus: a member of a novel group of RNA viruses that infect insects. Arch. Virol. 10(144):2051-2058
IRESs:
IRES:
Version: 2 Last change: 2009-08-29 21:31:37
Originaly submitted by: Martin Mokrejš Reviewed by: Martin Mokrejš
The IRES name:
  HiPV_IGRpred
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  6286-6473
Conclusion:
  putative_IRES
How IRES boundaries were determined:
experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
The IRES was NOT characterized experimentally.
Citations:
Nishiyama T, Yamamoto H, Uchiumi T, Nakashima N (2007) Eukaryotic ribosomal protein RPS25 interacts with the conserved loop region in a dicistroviral intergenic internal ribosome entry site. Nucleic Acids Res. 5(35):1514-1521
Last change to the database: 2015-04-16 16:45:23 GMT+1