The nucleic acid data:
IRESite Id: 67 Version: 2
Originaly submitted by: Petra Sekyrová Submission date: 2005-08-08 00:00:00
Reviewed by: Martin Mokrejš Last change: 2006-04-28 00:00:00
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  possibly_wrong
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  Hsp70Aa
The genetic origin of this natural mRNA/+RNA:
  nuclear
The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
24646134
Synonyms of the gene name:
Synonym: 87A
Synonym: hs70
Synonym: Hsp 70
Synonym: Hsp70A
Synonym: dhsp70
Synonym: hsp-70
Synonym: CG31366
Synonym: hsp70Aa
Synonym: hsp70 87A
Synonym: Protein 70
Synonym: hsp70(87A)
Synonym: heat shock 70
The mRNA/+RNA description: 
Drosophila melanogaster CG31366-PA (Hsp70Aa) mRNA, complete cds.
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  guessed_as_the_sequence_was_never_published_by_authors_nor_described_in_sufficient_detail
The organism containing this mRNA with IRES segment in its genome:
Drosophila melanogaster
A promoter reported in cDNA corresponding to IRES sequence:
  not tested
The total number of notable open-reading frames (ORFs):
  1
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 0
Originaly submitted by: Petra Sekyrová Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
Hsp70Aa
The description of the protein encoded in this ORF:
Drosophila heat shock protein 70
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  no
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  504-2432
OPTIONAL: The function of the encoded protein
Drosophila heat shock protein 70
Remarks:
From the reference (Hernandez et al. 2004) is not clear, which isoform of Drosophila hsp-70 was used, thus
mRNA of hsp-70Aa isoform has been taken because of the longest 5'UTR sequence (all of 5'UTRs share strong
similarity).
Citations:
Hernandez G., Vazquez-Pianzola P., Sierra J. M., Rivera-Pomar R. (2004) Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos. RNA. 10(11):1783-1797
IRESs:
IRES:
Version: 6 Last change: 2008-05-13 21:43:16
Originaly submitted by: Petra Sekyrová Reviewed by: Martin Mokrejš
The IRES name:
  hsp70
Warning: please make ires_name same as the gene_name and optionally append to it coordinates. E.g. when gene/virus name is EMCV-R use EMCV-R_-222_to_-1 or EMCV-R_1-456, etc. but not Emcv-R-... or EMCV-222_to_-1. Please keep case of letters as well. This rewards when searching through the database.
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  1-503
Conclusion:
  putative_IRES
How IRES boundaries were determined:
guessed
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
Authors have not specified (except Fig. 3) the range of 5'-UTR tested, thus whole sequence 1-503 is annotated
here as putative IRES sequence. Possible promoter existence was eliminated for reaper 5'-UTR, which is similar
to hsp70 and thus authors extrapolated the conclusion also to hsp70.

Integrity of bicistronic constructs used in vivo was tested by Northern blot. Integrity of the in vitro
transcripts was monitored using traces of 32P-labeled co-reporter RNA. Most of the experiments were performed
using reaper and not hsp70 UTR and utilized in vitro translation of capped/uncapped monocistronic mRNAs.
Citations:
Hernandez G., Vazquez-Pianzola P., Sierra J. M., Rivera-Pomar R. (2004) Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos. RNA. 10(11):1783-1797
Last change to the database: 2015-04-16 16:45:23 GMT+1