The nucleic acid data:
IRESite Id: 83 Version: 5
Originaly submitted by: Václav Vopálenský Submission date: 2005-10-26 00:00:00
Reviewed by: Martin Mokrejš Last change: 2006-08-10 00:00:00
IRESite record type:
  natural_transcript
The shape of the nucleic acid molecule translated:
  linear
The quality of the mRNA/+RNA sequence:
  end-to-end_full-length_mRNA
The abbreviated name of the virus/gene coding for this mRNA/+RNA molecule:
  hAT1R-A
The genetic origin of this natural mRNA/+RNA:
  nuclear
The GenBankId GI:# number of exactly this mRNA/+RNA sequence:
14043067
Synonyms of the gene name:
Synonym: AGTR1
Synonym: AG2S
Synonym: AT1B
Synonym: AT2R1
Synonym: HAT1R
Synonym: AGTR1A
Synonym: AGTR1B
Synonym: AT2R1A
Synonym: AT2R1B
Synonym: AT1
The mRNA/+RNA description: 
Homo sapiens angiotensin II receptor, type 1 (AGTR1), transcript variant 2, mRNA.
The mRNA/+RNA sequence represented in the +DNA notation:


Credibility of mRNA sequence:
  guessed_as_the_sequence_was_never_published_by_authors_nor_described_in_sufficient_detail
The organism containing this mRNA with IRES segment in its genome:
Homo sapiens
A promoter reported in cDNA corresponding to IRES sequence:
  no
The total number of notable open-reading frames (ORFs):
  1
Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:
Summary of experiments studying integrity of the in vivo transcripts in a particular host:
Integrity (uniformity) of mRNA tested using Northern-blot:
homogeneous_population_of_molecules_confirmed
Integrity (uniformity) of mRNA tested using RNase protection:
not_tested
Integrity (uniformity) of mRNA tested using 5'-RACE:
not_tested
Integrity (uniformity) of mRNA tested using primer extension :
not_tested
Integrity (uniformity) of mRNA tested using RT-PCR:
not_tested
Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR):
not_tested
Integrity (uniformity) of mRNA tested using RNAi:
not_tested
Integrity (uniformity) of mRNA tested using S1 nuclease mapping:
not_tested
Cryptic promoter presence was confirmed by expression from a promoter-less plasmid:
no_promoter_confirmed
Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter:
not_tested
Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks:
not_tested
The organism used:
Homo sapiens NCI-H295 [H295] [H295-R] (ATCC CRL-10296)
Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:
ORF
ORF position:   1
Version: 2
Originaly submitted by: Václav Vopálenský Reviewed by: Martin Mokrejš
The abbreviated name of this ORF/gene:
AGTR1
The description of the protein encoded in this ORF:
angiotensin II receptor, type 1
The translational frameshift (ribosome slippage) involved:
  0
The ribosome read-through involved:
  no
The alternative forms of this protein occur by the alternative initiation of translation:
  no
The ORF absolute position (the base range includes START and STOP codons or their equivalents):
  298-1377
OPTIONAL: The function of the encoded protein
GO:0005886; Cellular component: plasma membrane
GO:0016021; Cellular component: integral to membrane
GO:0005887; Cellular component: integral to plasma membrane
GO:0004872; Molecular function: receptor activity
GO:0004947; Molecular function: bradykinin receptor activity
GO:0001584; Molecular function: rhodopsin-like receptor activity
GO:0016494; Molecular function: C-X-C chemokine receptor activity
GO:0004945; Molecular function: angiotensin type II receptor activity
GO:0007589; Biological process: fluid secretion
GO:0008015; Biological process: circulation
GO:0007165; Biological process: signal transduction
GO:0007204; Biological process: elevation of cytosolic calcium ion concentration
GO:0007186; Biological process: G-protein coupled receptor protein signaling pathway
GO:0007200; Biological process: G-protein signaling, coupled to IP3 second messenger (phospholipase C
activating)
Remarks:
Integrity of the bicistronic transcripts transcribed in vivo was verified by Northern blot analysis using cDNA
probes against firefly luciferase. Cryptic promoter existence within the AT1R 5'-UTR was ruled out using a
promoter-less plasmid and subsequent measurement of luciferase activity in cells (1/30 of the positive control
containing SV40 promoter and enhancer).
Citations:
Martin M. M., Garcia J. A., McFarland J. D., Duffy A. A., Gregson J. P., Elton T. S. (2003) Translation of the human angiotensin II type 1 receptor mRNA is mediated by a highly efficient internal ribosome entry site. Mol. Cell. Endocrinol. 212(1-2):51-61
IRESs:
IRES:
Version: 4 Last change: 2007-01-11 00:00:00
Originaly submitted by: Václav Vopálenský Reviewed by: Martin Mokrejš
The IRES name:
  AT1R_var2
Warning: please make ires_name same as the gene_name and optionally append to it coordinates. E.g. when gene/virus name is EMCV-R use EMCV-R_-222_to_-1 or EMCV-R_1-456, etc. but not Emcv-R-... or EMCV-222_to_-1. Please keep case of letters as well. This rewards when searching through the database.
The IRES absolute position (the range includes START and STOP codons or their equivalents):
  26-297
Conclusion:
  putative_IRES
How IRES boundaries were determined:
guessed
The sequence of IRES region aligned to its secondary structure (if available):


Remarks:
AGTR1 5' UTR from nt -272 to nt -1
Citations:
Martin M. M., Garcia J. A., McFarland J. D., Duffy A. A., Gregson J. P., Elton T. S. (2003) Translation of the human angiotensin II type 1 receptor mRNA is mediated by a highly efficient internal ribosome entry site. Mol. Cell. Endocrinol. 212(1-2):51-61
Last change to the database: 2015-04-16 16:45:23 GMT+1