IRESite_Id:216 promoter-less plasmid (deltaSV40)RSTF-LEF1-1.2 used to evaluate potential promoter activity in LEF1 IRES

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `216`	Version: `5`
Originaly submitted by: Martin Mokrejš
Reviewed by: Martin Mokrejš	Last change: `2007-01-23 00:00:00`

IRESite record type:

promoter-less_plasmid_with_putative_IRES

The name of the plasmid:
(deltaSV40)RSTF-LEF1-1.2

Description of the plasmid (facultative for promoter-less plasmid records):
The promoter-less plasmid was created by insertion of PCR-based product of 1.178kb of the LEF1 5'-UTR blunt-end ligated into (deltaSV40)pRSTF plasmid backbone linearized in the AfeI (Eco47III) site AGC^GCT.

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

no

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

no

A promoter reported in cDNA corresponding to IRES sequence:

no

Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:

Summary of experiments studying integrity of the in vivo transcripts in a particular host:

Integrity (uniformity) of mRNA tested using Northern-blot:
homogeneous_population_of_molecules_confirmed

Integrity (uniformity) of mRNA tested using RNase protection:
not_tested

Integrity (uniformity) of mRNA tested using 5'-RACE:
not_tested

Integrity (uniformity) of mRNA tested using primer extension :
not_tested

Integrity (uniformity) of mRNA tested using RT-PCR:
not_tested

Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR):
not_tested

Integrity (uniformity) of mRNA tested using RNAi:
not_tested

Integrity (uniformity) of mRNA tested using S1 nuclease mapping:
not_tested

Cryptic promoter presence was confirmed by expression from a promoter-less plasmid:
no_promoter_confirmed

Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter:
not_tested

Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks:
not_tested

The organism used:

Cercopithecus aethiops COS-1 (ATCC CRL-1650)

The origin of IRES in the plasmid:

nuclear

The donor organism of the IRES segment:

Homo sapiens

The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
LEF1

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

agatcgatct gcgcagcacc atgctagcca ccatGacttc gaaagtttat gatccagaac aaaggaaacg gatgataact ggtccgcagt ggtgggccag 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

atgtaaacaa atgaatgttc ttgattcatt tattaattat tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca gaccttattg gtatgggcaa atcaggcaaa tctggtaatg 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gttcttatag gttacttgat cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt gtcggccatg attggggtgc 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ttgtttggca tttcattata gctatgagca tcaagataag atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa aatggttttg gagaataact tcttcgtgga aaccatgttg ccatcaaaaa 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

tcatgagaaa gttagaacca gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

cccgttagta aaaggtggta aacctgacgt tgtacaaatt gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat gtttattgaa 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt tcctaatact gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

atgcacctga tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaaatGtc gaTCGACggg ggcccctagg AgatccAAGC 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

TTATCGATTT CGAACCCGGG GTACCGAATT CCTCGAGTCT AGAGGAgcat gcTCCTCTAG ACTCGAGGAA TTCGGTACCC CGGGTTCGAA ATCGATAAGC 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

TTgGATCTag cGCGCGGGAG GAGGAGAAGC AGTGGGGAGG CGCAGCCGCT CAcCTGCGGG GCAGGGCGCG GAGGAGGGAC CCGGNTGcGc GCTCTCGGGC 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

CGAGGAACCA GGACGcGCCC GGAGCCTCGC ACGCGGCCAA GCTCGGGCGT CCCCTCCCCT CGGCCGGGCG AACTCAAGGG GCGCAGCTCT TTGCTTTGAC 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

AGAGCTGGCC GGCGGAGGCG TGCAGAGCGG CGAGCCGGCG AGCCAGGCTG AGAAACTCGA CCCGGGAACA AAGAGGGGTC GGACTGAGTG TGTGTGTCGG 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

CTCGAGCTCC GGGNAGAGGC ATTTGCCCGA GGNCCCCGCT GTGACTCCCC GAGACTCCGC AGTGCCCTCC ACTGNGGAGT CCCCGCGCTT GCCGGNAAAA 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

ACTTTATTCT TGGCAAACTT CTCTTTCTCT TCCCCTCCTC CTCGGCCCCC ATCTTCTGCT CCTCCTCCTT CTCTAGCAGA TTAAATGAGC CTCGAGAAGA 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

AAAACCGAAG CGAAAGGGAA GAAAATAAGA AGATCTAAAA CGGACATCTC CAGCGTGGGT GGCTCCTTTT TCTTTTTCTT TTTTTCCCAC CCTTCAGGAA 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

GTGGACGTTT CGTTATCTTC TGATCCTTGC ACCTTCTTTT GGGGCAAACG GGGCCCTTCT GCCCAGATCC CCTCTCTTTT CTCGGAAAAC AAACTACTAA 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

GTCGGCATCC GGGGTAACTA CAGTGGAGAG GGTTTCCGCG GAGACGCGCC GCCGGACCCT CCTCTGCACT TTGGGGAGGC GTGCTCCCTC CAGAACCGGC 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

GTTCTCCGCG CGCAAATCCC GGCGACGCGG GGTCGCGGGG TGGCCGCCGG GGCAGCCTCG TCTAGCGCGC GCCGCGCAGA CGCCCCCGGA GTCGCCAGCT 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

ACCGCAGCCC TCGCCGCCCA GTGCCCTTCG GCCTCGGGGC GGGCGCCTGC GTCGGTCTCC GCGAAGCGGG AAAGCGCGGC GGCCGCCGGG ATTCGGGCGC 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

CGCGGCAGCT GCTCCGGCTG CCGGCCGGCG GCCCCGCGCT CGCCCGCCCC GCTTCCGCCC GCTGTCCTGC TGCACGAACC CTTCCAACTC TCCTTTCCTC 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

CCCCACCCTT GAGTTACCCC TCTGTCTTTC CTGCTGTTGC GCGGGTGCTC CCACAGCGGA GCGGAGATTA CAGAGCCGCC GGGATGCCCg ctGGATCCAT 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

GGaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta atTCTAGAgc ggccgcttcg agcagacatg ataagataca ttgatgagtt 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

ataagtttaa acagatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

ggccttttgc tggccttttg ctcacatggc tcgac
^6001      ^6011      ^6021      ^6031

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

The total number of notable open-reading frames (ORFs):

2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
RLuc

The description of the protein encoded in this ORF:
Renilla luciferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

33-989

ORF

ORF position:

2

Version: `0`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
FLuc-fusion

The description of the protein encoded in this ORF:
Firefly-luciferase protein with one additional amoniacid residues at its N-terminus

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

2299-3951

Remarks:

Authentic RNA pol II promoter was confirmed between bases +280 and +640 relative to the main start site of
transcription. Comment from J. Jimenez: Our original studies of the LEF1 5'UTR were based on characterizing
a third promoter in the 5'UTR that is responsible for generating a 3.0 kb transcript. Because we had evidence
of an authentic promoter, we performed N. blot analysis. We did also attempt RT-PCR, but had technical
problems due to the high GC content of the 5'UTR.

Citations:

Jimenez J., Jang G. M., Semler B. L., Waterman M. L. (2005) An internal ribosome entry site mediates translation of lymphoid enhancer factor-1. RNA. 11(9):1385-1399

IRESs:

IRES:

Version: `1`	Last change: `2007-01-06 00:00:00`
Originaly submitted by: Martin Mokrejš	Reviewed by: Martin Mokrejš
The IRES name: `LEF1`	The functional status of IRES: `functional`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

1112-2289

How IRES boundaries were determined:
experimentally_determined

5'-end of IRES relative to last base of the STOP codon of the upstream ORF: `123`	3'-end of IRES relative to last base of the STOP codon of the upstream ORF: `1300`
5'-end of IRES relative to first base of the START codon of the downstream ORF: `-1187`	3'-end of IRES relative to first base of the START codon of the downstream ORF: `-10`

The sequence of IRES region aligned to its secondary structure (if available):

GCGCGGGAGG AGGAGAAGCA GTGGGGAGGC GCAGCCGCTC AcCTGCGGGG CAGGGCGCGG AGGAGGGACC CGGNTGcGcG CTCTCGGGCC GAGGAACCAG 
^1112      ^1122      ^1132      ^1142      ^1152      ^1162      ^1172      ^1182      ^1192      ^1202      

GACGcGCCCG GAGCCTCGCA CGCGGCCAAG CTCGGGCGTC CCCTCCCCTC GGCCGGGCGA ACTCAAGGGG CGCAGCTCTT TGCTTTGACA GAGCTGGCCG 
^1212      ^1222      ^1232      ^1242      ^1252      ^1262      ^1272      ^1282      ^1292      ^1302      

GCGGAGGCGT GCAGAGCGGC GAGCCGGCGA GCCAGGCTGA GAAACTCGAC CCGGGAACAA AGAGGGGTCG GACTGAGTGT GTGTGTCGGC TCGAGCTCCG 
^1312      ^1322      ^1332      ^1342      ^1352      ^1362      ^1372      ^1382      ^1392      ^1402      

GGNAGAGGCA TTTGCCCGAG GNCCCCGCTG TGACTCCCCG AGACTCCGCA GTGCCCTCCA CTGNGGAGTC CCCGCGCTTG CCGGNAAAAA CTTTATTCTT 
^1412      ^1422      ^1432      ^1442      ^1452      ^1462      ^1472      ^1482      ^1492      ^1502      

GGCAAACTTC TCTTTCTCTT CCCCTCCTCC TCGGCCCCCA TCTTCTGCTC CTCCTCCTTC TCTAGCAGAT TAAATGAGCC TCGAGAAGAA AAACCGAAGC 
^1512      ^1522      ^1532      ^1542      ^1552      ^1562      ^1572      ^1582      ^1592      ^1602      

GAAAGGGAAG AAAATAAGAA GATCTAAAAC GGACATCTCC AGCGTGGGTG GCTCCTTTTT CTTTTTCTTT TTTTCCCACC CTTCAGGAAG TGGACGTTTC 
^1612      ^1622      ^1632      ^1642      ^1652      ^1662      ^1672      ^1682      ^1692      ^1702      

GTTATCTTCT GATCCTTGCA CCTTCTTTTG GGGCAAACGG GGCCCTTCTG CCCAGATCCC CTCTCTTTTC TCGGAAAACA AACTACTAAG TCGGCATCCG 
^1712      ^1722      ^1732      ^1742      ^1752      ^1762      ^1772      ^1782      ^1792      ^1802      

GGGTAACTAC AGTGGAGAGG GTTTCCGCGG AGACGCGCCG CCGGACCCTC CTCTGCACTT TGGGGAGGCG TGCTCCCTCC AGAACCGGCG TTCTCCGCGC 
^1812      ^1822      ^1832      ^1842      ^1852      ^1862      ^1872      ^1882      ^1892      ^1902      

GCAAATCCCG GCGACGCGGG GTCGCGGGGT GGCCGCCGGG GCAGCCTCGT CTAGCGCGCG CCGCGCAGAC GCCCCCGGAG TCGCCAGCTA CCGCAGCCCT 
^1912      ^1922      ^1932      ^1942      ^1952      ^1962      ^1972      ^1982      ^1992      ^2002      

CGCCGCCCAG TGCCCTTCGG CCTCGGGGCG GGCGCCTGCG TCGGTCTCCG CGAAGCGGGA AAGCGCGGCG GCCGCCGGGA TTCGGGCGCC GCGGCAGCTG 
^2012      ^2022      ^2032      ^2042      ^2052      ^2062      ^2072      ^2082      ^2092      ^2102      

CTCCGGCTGC CGGCCGGCGG CCCCGCGCTC GCCCGCCCCG CTTCCGCCCG CTGTCCTGCT GCACGAACCC TTCCAACTCT CCTTTCCTCC CCCACCCTTG 
^2112      ^2122      ^2132      ^2142      ^2152      ^2162      ^2172      ^2182      ^2192      ^2202      

AGTTACCCCT CTGTCTTTCC TGCTGTTGCG CGGGTGCTCC CACAGCGGAG CGGAGATTAC AGAGCCGCCG GGATGCCC
^2212      ^2222      ^2232      ^2242      ^2252      ^2262      ^2272      ^2282

Citations:

Jimenez J., Jang G. M., Semler B. L., Waterman M. L. (2005) An internal ribosome entry site mediates translation of lymphoid enhancer factor-1. RNA. 11(9):1385-1399

Last change to the database: 2019-03-18 09:32:49 GMT+1