Date Reason login IRESite Id Gene name IRES name IRES functional status mRNA status GI:# description recordtype ORF count IRES start IRES stop Translation method in-vitro transl. system Translation effic. chng. in % of new IRES Translation effic. chng. in % of positive control IRES Translation effic. chng. in % of negative control IRES Posit. control. IRES name mRNA characteristics IRESite Seq Id IRESite IRES Id IRESite IRES Seq Id IRESite IRES Struct Id translation id protein interaction id IRESite 2DExp Id rrna complementarity id
2019-03-18 09:32:49 changed ismaeil 640 HCV 4a ['HCV-IRES-4a-Egyptain-Patients-TBRI'] [None] only_IRES_fragment 1333190374 ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Hepacivirus. natural_transcript [1] [265] [] [] [] [] [] [] [] 2045 [760L] [2046L] [69L] [] [] [51L] []
2019-03-18 09:25:55 submitted ismaeil 640 HCV 4a ['HCV-IRES-4a-Egyptain-Patients-TBRI'] [None] only_IRES_fragment 981540 ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Hepacivirus. natural_transcript [1] [265] [] [] [] [] [] [] [] 2045 [760L] [2046L] [69L] [] [] [51L] []
2015-04-16 16:45:23 submitted jestall 639 [] [] promoter-less_plasmid_without_putative_IRES 2 [] [] [] [] [] [] [] [] [] [] [] [] [] [] [] []
2015-04-08 23:31:45 changed mmokrejs 110 Apaf-1 ['Apaf-1'] [None] hopefully_full-length_mRNA Homo sapiens apoptotic protease activating factor 1 (Apaf-1) mRNA, complete cds. Similar to C. elegans cell death gene ced-4. natural_transcript 1 [504] [734] [] [] [] [] [] [] [] 2008 [117L] [1130L] [43L] [] [33L] [26L] []
2011-04-08 22:43:22 changed mmokrejs 638 ['HCV_type_1b_1-341', 'EMCV-R_260-827'] ['functional', 'functional'] end-to-end_full-length_mRNA 5441834 Hepatitis C virus replicon I377/NS3-3'UTR. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1, 1190] [341, 1800] [] [] [] [] [] [] [] 2042 [758L, 759L] [301L, 2043L] [None, None] [] [] [] []
2011-04-08 22:34:23 changed mmokrejs 349 ['EMCV-R_260-832'] ['functional'] hopefully_full-length_mRNA Putative spliced bicistronic mRNA transcript derived from pRF based plasmid with EMCV-R IRES in the intercistronic region encoding Fluc-fusion protein extended by 2 aminoacid residues. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1168] [1745] [] [] [] [] [] [] [] 1175 [496L] [1177L] [None] [] [] [] []
2011-04-08 22:32:43 changed mmokrejs 230 ['EMCV-R_260-832'] ['functional'] our_best_guess Putative bicistronic in vitro T7 transcript (till the cleaved BamHI site) containing EMCV-R IRES located between RLuc and FLuc genes. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1026] [1603] [] [] [] [] [] [] [] 1174 [382L] [782L] [None] [] [] [] []
2011-04-08 22:31:31 changed mmokrejs 227 ['EMCV-R_260-832'] ['functional'] our_best_guess Putative spliced bicistronic mRNA transcript derived from pRF based plasmid with EMCV-R IRES in the intercistronic region. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1168] [1745] [] [] [] [] [] [] [] 1172 [379L] [782L] [None] [] [] [] []
2011-04-08 22:29:43 changed mmokrejs 382 ['EMCV-R_260-845'] ['functional'] our_best_guess One of the two putative in vivo transcripts of mammalian expression vector pNBioSec: this one is from minimal CMV promoter down to beta-globin poly(A) signal from rabbit. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 1 [359] [945] [] [] [] [] [] [] [] 1273 [520L] [1271L] [None] [] [] [] []
2011-04-08 22:28:06 changed mmokrejs 384 ['EMCV-R_260-845'] ['functional'] our_best_guess One of the two putative in vivo transcripts of mammalian expression vector pNBio: this one is from minimal CMV promoter down to beta-globin poly(A) signal from rabbit. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 1 [282] [868] [] [] [] [] [] [] [] 1275 [521L] [1271L] [None] [] [] [] []
2011-04-08 22:26:36 changed mmokrejs 379 ['EMCV-R_260-845'] ['functional'] both_UTRs_possibly_incomplete Putative in vivo transcript of cloning vector pLXRN from Mo-MuLV down to the end of R in right LTR. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 1 [717] [1303] [] [] [] [] [] [] [] 1266 [518L] [1268L] [None] [] [] [] []
2011-04-08 22:24:46 changed mmokrejs 386 ['EMCV-R_260-845'] ['functional'] possibly_wrong Putative in vivo spliced transcript of pIREShyg plasmid from CLONTECH with synthetic intron spliced out. The intron boundaries do not conform the GT-AG consensus. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 1 [146] [732] [] [] [] [] [] [] [] 1277 [522L] [1268L] [None] [] [] [] []
2011-04-08 22:22:20 changed mmokrejs 582 ['EMCV-R_245-832'] ['functional'] our_best_guess Incomplete T3 promoter driven monocistronic transcript of pIRES-lacZ plasmid preceded by EMCV-R IRES. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 1 [71] [658] [] [] [] [] [] [] [] 1861 [706L] [1863L] [None] [] [] [] []
2011-04-08 22:21:15 changed mmokrejs 638 ['HCV_type_1b', 'EMCV-R_260-827'] ['functional', 'functional'] end-to-end_full-length_mRNA 5441834 Hepatitis C virus replicon I377/NS3-3'UTR. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1, 1190] [341, 1800] [] [] [] [] [] [] [] 2042 [758L, 759L] [301L, 2043L] [None, None] [] [] [] []
2011-04-08 22:19:39 changed mmokrejs 547 ['EMCV-R_281-833'] ['functional'] hopefully_full-length_mRNA In vitro T7 promoter based HpaI site run-off transcript of pF/R-wt plasmid. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1741] [2293] [] [] [] [] [] [] [] 1762 [679L] [1761L] [None] [] [] [] []
2011-04-08 22:18:55 changed mmokrejs 546 ['EMCV-R_281-833'] ['functional'] 3UTR_possibly_incomplete Putative in vivo CMV IE promoter-derived spliced transcript contain firefly luciferase and renilla luciferase separated by a wild-type EMCV IRES up to the SV40 poly(A) signal. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1948] [2500] [] [] [] [] [] [] [] 1759 [678L] [1761L] [None] [] [] [] []
2011-04-08 22:17:34 changed mmokrejs 460 ['EMCV-R_259-825'] ['functional'] hopefully_full-length_mRNA In vivo CMV transcript produced from bicistronic plasmid pDC-EMCV which encodes neomycin phosphotransferase and beta-galactosidase as the first and the second cistron respectively and the EMCV-R IRES (nt from -575 to nt -9 of the original sequence) inserted in between reporters. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1110] [1676] [] [] [] [] [] [] [] 1509 [594L] [1385L] [None] [] [] [] []
2011-04-08 22:15:35 changed mmokrejs 428 ['EMCV-R_259-825'] ['functional'] hopefully_full-length_mRNA In vitro T7 run-off transcript produced from bicistronic plasmid pDC-EMCV which encodes neomycin phosphotransferase and truncated version of the beta-galactosidase (linearized at the Ssp1 site at position 3744 within pDC-EMCV; see 1384.gb below) as the first and the second cistron respectively and the EMCV-R IRES (nt from -575 to nt -9 of the original sequence) inserted between reporters. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1059] [1625] [] [] [] [] [] [] [] 1383 [561L] [1385L] [None] [] [] [] []
2011-04-08 22:14:11 changed mmokrejs 292 ['EMCV-R_281-827'] ['functional'] hopefully_full-length_mRNA Putative T7 promoter-derived transcript transcribed either in vivo or in vitro in BHK-21 cells infected by recombinant vaccinia virus vTF7-3 containing 5'-EMCV-R_IRES-CAT-EMCV_type_R_IRES_eGFP-3'. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1289] [1835] [] [] [] [] [] [] [] 948 [441L] [950L] [None] [] [] [] []
2011-04-08 22:13:01 changed mmokrejs 545 ['EMCV-R_335-834'] ['functional'] hopefully_full-length_mRNA Putative in vitro transcript from an empty vector from T7 promoter up to Tphi transcription terminator containing 30 nt long poly(A) stretch after the ORF but not at the physical 3'-end of the transcript. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 1 [17] [516] [] [] [] [] [] [] [] 1756 [677L] [1758L] [None] [] [] [] []
2011-04-08 22:11:58 changed mmokrejs 544 ['EMCV-R_335-834'] ['functional'] 3UTR_incomplete Putative in vitro T7 promoter derived transcript of the empty vector up to the linearization PstI site. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 1 [17] [516] [] [] [] [] [] [] [] 1753 [676L] [1755L] [None] [] [] [] []
2011-04-08 22:10:28 changed mmokrejs 380 ['EMCV-R_260-845'] ['functional'] both_UTRs_possibly_incomplete One of the two putative in vivo transcripts of mammalian expression vector pCBio: this one is from minimal CMV promoter down to beta-globin poly(A) signal from rabbit. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 1 [221] [807] [] [] [] [] [] [] [] 1269 [519L] [1271L] [None] [] [] [] []
2011-04-08 22:08:17 changed mmokrejs 263 ['EMCV-R_261-845'] ['functional'] our_best_guess Putative in vivo CMV transcript of pCAT-EMCV-eGFP including the SV40 early poly(A) signal. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [814] [1400] [] [] [] [] [] [] [] 868 [413L] [870L] [None] [] [] [] []
2011-04-08 22:03:26 changed mmokrejs 245 ['EMCV-R_271-831'] ['functional'] 3UTR_incomplete Putative bicistronic mRNA transcript derived from pBSRLucEFluc plasmid through SV40 promoter. The plasmid has in the intercistronic region T7 promoter. The FLuc ORF has one additional aminoacid and one putative sequencing error. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1188] [1749] [] [] [] [] [] [] [] 829 [395L] [831L] [None] [] [] [] []
2011-04-08 22:02:13 changed mmokrejs 326 ['EMCV-R_272-831'] ['functional'] 3UTR_incomplete Putative bicistronic mRNA transcript derived from pBSRLucEFluc plasmid through SV40 promoter. The plasmid has in the intercistronic region T7 promoter. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [2072] [2633] [] [] [] [] [] [] [] 1054 [478L] [1056L] [None] [] [] [] []
2011-04-08 21:56:24 changed mmokrejs 585 ['EMCV-R_260-831'] ['functional'] our_best_guess In vivo spliced transcript derived through CMV IE promoter containing Renilla and firefly luciferases and terminated after the SV40 late poly(A) signal. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [1250] [1829] ['in vivo', 'in vivo'] [None, None] ['100.000', '100.000'] ['None', 'None'] ['25.000', '18.000'] [None, None] ['endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 1868 [708L] [1870L] [None] [619L, 620L] [] [] []
2011-04-08 21:51:24 changed mmokrejs 353 ['EMCV-R_260-841'] ['functional'] end-to-end_full-length_mRNA In vitro T3 run-off transcript containing 5' region of EMCV-R containing the IRES (-574 to -1) plus the first 8 nucleotides from the viral polyprotein cloned upstream of the luciferase reporter RNA lacking a poly(A) tail. plasmid_with_promoter_and_putative_IRES_translationally_characterized 1 [62] [644] ['in vitro'] ['HeLa cell lysate'] ['100.000'] ['None'] ['0.003'] [None] ['exogenous_RNA_with_ApppG_cap_without_polyA_tail'] 1192 [500L] [1187L] [None] [424L] [] [] []
2011-04-08 21:49:13 changed mmokrejs 354 ['EMCV-R_260-841'] ['functional'] end-to-end_full-length_mRNA In vitro T3 run-off transcript containing 5' region of EMCV-R containing the IRES (-574 to -1) plus the first 8 nucleotides from the viral polyprotein cloned upstream of the luciferase reporter RNA with a poly(A) tail. plasmid_with_promoter_and_putative_IRES_translationally_characterized 1 [62] [644] ['in vitro'] ['HeLa cell lysate'] ['100.000'] ['None'] ['0.050'] [None] ['exogenous_RNA_with_ApppG_cap_with_polyA_tail'] 1193 [501L] [1187L] [None] [425L] [] [] []
2011-04-08 21:47:14 changed mmokrejs 585 ['EMCV-R_260-831'] ['functional'] our_best_guess In vivo spliced transcript derived through CMV IE promoter containing Renilla and firefly luciferases and terminated after the SV40 late poly(A) signal. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [1250] [1829] ['in vivo', 'in vivo'] [None, None] ['100.000', '100.000'] ['None', 'None'] ['25.000', '18.000'] [None, None] ['endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 1868 [708L] [1870L] [None] [619L, 620L] [] [] []
2011-04-08 21:41:58 changed mmokrejs 590 ['EMCV-R_260-831'] ['functional'] hopefully_full-length_mRNA In vivo spliced monocistronic CMV IE promoter driven transcript containing EMCV-R IRES in its 5'-UTR and terminated after the SV40 poly(A) signal. plasmid_with_promoter_and_putative_IRES_translationally_characterized 1 [254] [833] ['in vivo'] [None] ['100.000'] ['None'] ['120.000'] [None] ['endogenous_nuclear_RNA_Pol_II_transcript'] 1877 [711L] [1870L] [None] [623L] [] [] []
2011-04-08 21:40:56 changed mmokrejs 590 ['EMCV-R_260-831'] ['functional'] hopefully_full-length_mRNA In vivo spliced monocistronic CMV IE promoter driven transcript containing EMCV-R IRES in its 5'-UTR and terminated after the SV40 poly(A) signal. plasmid_with_promoter_and_putative_IRES_translationally_characterized 1 [254] [833] ['in vivo'] [None] ['100.000'] ['None'] ['120.000'] [None] ['endogenous_nuclear_RNA_Pol_II_transcript'] 1877 [711L] [1870L] [None] [623L] [] [] []
2011-04-08 21:37:11 changed mmokrejs 608 ['EMCV-R_315-845'] ['functional'] both_UTRs_incomplete Part of the T7-derived transcript studied in in vitro assays. The 5'-UTR could have been longer but do not know the necessary details. The intercistronic region contains 5-UTR sequence of EMCV-R virus (bp 315-843). plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [785] [1315] ['in vivo', 'in vitro', 'in vitro'] [None, 'rabbit reticulocytes lysate', 'wheat germ lysate'] ['100.000', '279.000', '100.000'] ['None', 'None', 'None'] ['0.860', '1.000', '80.000'] [None, None, None] ['endogenous_cytoplasmic_uncapped_T7_transcript_without_polyA_tail', 'exogenous_RNA_without_cap_without_polyA_tail', 'exogenous_RNA_without_cap_without_polyA_tail'] 1930 [726L] [1931L] [None] [634L, 635L, 636L] [] [] []
2010-11-30 21:33:39 changed mmokrejs 57 AML1/RUNX1 ['AML1/RUNX1'] [None] 5UTR_possibly_incomplete 49574545 Homo sapiens runt-related transcription factor 1 (acute myeloid leukemia 1; aml1 oncogene) (RUNX1), transcript variant 2, mRNA. Poly(A)-tail sequence dropped. natural_transcript 1 [39] [1599] [] [] [] [] [] [] [] 2001 [57L] [147L] [None] [] [] [] []
2010-03-03 23:22:41 changed mmokrejs 596 SV40 ['SV40_661-830'] [None] end-to-end_full-length_mRNA The SV40 late 19S long-spliced mRNA derived from transcription start site in genomic position 325. This is the most abundant variant (11% relative of all early and late mRNAs) of the 19S late transcripts. natural_transcript 4 [153] [322] [] [] [] [] [] [] [] 1895 [717L] [1896L] [None] [] [] [] []
2010-01-12 21:46:05 changed mmokrejs 55 n-MYC ['n-MYC'] [None] our_best_guess Homo sapiens v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN), mRNA. Poly(A) tail dropped. natural_transcript 1 [8] [327] [] [] [] [] [] [] [] 2000 [55L] [143L] [None] [] [] [] []
2009-10-13 13:41:30 changed mmokrejs 574 eIF4GII ['eIF4GII-long'] [None] hopefully_full-length_mRNA Eukaryotic translation initiation factor 4 gamma, isoform II, long. Poly(A)-tail sequence was dropped. natural_transcript 1 [288] [614] [] [] [] [] [] [] [] 2023 [699L] [1840L] [None] [] [] [] []
2009-10-13 13:40:28 changed mmokrejs 573 eIF4GII ['eIF4GII'] [None] hopefully_full-length_mRNA 9967556 Homo sapiens eIF4GII mRNA, complete cds. Poly(A)-tail sequence was dropped. natural_transcript 1 [1] [256] [] [] [] [] [] [] [] 2020 [698L] [1838L] [None] [] [] [] []
2009-10-13 13:22:20 changed mmokrejs 574 eIF4GII ['eIF4GII-long'] [None] hopefully_full-length_mRNA Eukaryotic translation initiation factor 4 gamma, isoform II, long. Poly(A)-tail sequence was dropped. natural_transcript 1 [288] [614] [] [] [] [] [] [] [] 2023 [699L] [1840L] [None] [] [] [] []
2009-10-13 13:22:14 changed mmokrejs 573 eIF4GII ['eIF4GII'] [None] hopefully_full-length_mRNA 9967556 Homo sapiens eIF4GII mRNA, complete cds. Poly(A)-tail sequence was dropped. natural_transcript 1 [1] [256] [] [] [] [] [] [] [] 2020 [698L] [1838L] [None] [] [] [] []
2009-10-13 11:39:08 changed mmokrejs 548 eIF4G ['eIF4G'] [None] hopefully_full-length_mRNA 219612 Homo sapiens mRNA for eukaryotic initiation factor 4 gamma (eIF4G), as derived from the gamma promoter. natural_transcript 1 [1] [357] [] [] [] [] [] [] [] 1763 [680L] [1764L] [None] [] [] [] []
2009-10-13 11:13:32 changed mmokrejs 548 eIF4G ['eIF4G'] [None] hopefully_full-length_mRNA 219612 Homo sapiens mRNA for eukaryotic initiation factor 4 gamma (eIF4G), as derived from the gamma promoter. natural_transcript 1 [1] [357] [] [] [] [] [] [] [] 1763 [680L] [1764L] [None] [] [] [] []
2009-10-13 11:13:20 changed mmokrejs 576 eIF4GI ['eIF4GI-ext'] [None] hopefully_full-length_mRNA 156523974 Homo sapiens eukaryotic translation initiation factor 4 gamma (eIF4GI-ext), transcript variant 1, as derived from the beta promoter. Poly(A)-tail sequence was dropped. natural_transcript 1 [464] [659] [] [] [] [] [] [] [] 2019 [700L] [1843L] [None] [] [] [] []
2009-10-13 11:12:55 changed mmokrejs 574 eIF4GII ['eIF4GII-long'] [None] hopefully_full-length_mRNA Eukaryotic translation initiation factor 4 gamma, isoform II, long. Poly(A)-tail sequence was dropped. natural_transcript 1 [288] [614] [] [] [] [] [] [] [] 2023 [699L] [1840L] [None] [] [] [] []
2009-10-11 23:26:25 changed mmokrejs 110 Apaf-1 ['Apaf-1'] [None] hopefully_full-length_mRNA Homo sapiens apoptotic protease activating factor 1 (Apaf-1) mRNA, complete cds. Similar to C. elegans cell death gene ced-4. natural_transcript 1 [504] [734] [] [] [] [] [] [] [] 2008 [117L] [1130L] [43L] [] [33L] [26L] []
2009-10-10 02:32:48 changed mmokrejs 573 eIF4GII ['eIF4GII'] [None] hopefully_full-length_mRNA 9967556 Homo sapiens eIF4GII mRNA, complete cds. Poly(A)-tail sequence was dropped. natural_transcript 1 [1] [256] [] [] [] [] [] [] [] 2020 [698L] [1838L] [None] [] [] [] []
2009-10-10 02:09:05 changed mmokrejs 491 AQP4 ['AQP4'] [None] our_best_guess Human mercurial-insensitive water channel mRNA, form 1, complete cds. natural_transcript 1 [10] [293] [] [] [] [] [] [] [] 1608 [625L] [1606L] [41L] [] [] [24L] []
2009-09-18 13:13:41 changed mmokrejs 321 FMDV ['FMDV_type_C'] [None] end-to-end_full-length_mRNA 6318187 Foot-and-mouth disease virus (FMDV) strain C, isolate c-s8c1, genomic RNA. natural_transcript 1 [578] [1038] [] [] [] [] [] [] [] 1036 [471L] [1037L] [None] [] [40L, 24L, 41L, 42L, 43L, 44L, 45L, 46L] [45L] []
2009-09-17 18:07:52 submitted mmokrejs 638 ['HCV_type_1b', 'EMCV-R'] ['functional', 'functional'] end-to-end_full-length_mRNA 5441834 Hepatitis C virus replicon I377/NS3-3'UTR. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [1, 1190] [341, 1800] [] [] [] [] [] [] [] 2042 [758L, 759L] [301L, 2043L] [None, None] [] [] [] []
2009-09-17 18:03:35 reviewed mmokrejs 438 Cat-1 ['Cat-1_224'] [None] our_best_guess Rattus norvegicus cationic amino acid transporter-1 (Cat1) gene, merged from GI:18542255 and GI:33943114. natural_transcript 1 [47] [270] [] [] [] [] [] [] [] 1411 [571L] [1415L] [None] [] [] [] []
2009-09-17 15:12:51 changed mmokrejs 438 Cat-1 ['Cat-1_224'] [None] our_best_guess Rattus norvegicus cationic amino acid transporter-1 (Cat1) gene, merged from GI:18542255 and GI:33943114. natural_transcript 1 [47] [270] [] [] [] [] [] [] [] 1411 [571L] [1415L] [None] [] [] [] []
2009-09-17 12:50:48 changed mmokrejs 140 EMCV-R ['EMCV-R'] [None] end-to-end_full-length_mRNA Encephalomyocarditis virus, complete genome natural_transcript 1 [257] [832] [] [] [] [] [] [] [] 684 [156L] [685L] [None] [] [] [40L] []
2009-09-17 03:50:16 reviewed mmokrejs 637 EV71 ['EV71_1-748'] [None] end-to-end_full-length_mRNA 66967945 Human enterovirus 71 strain pinf7-54A from Taiwan, complete genome. natural_transcript 1 [1] [748] [] [] [] [] [] [] [] 2040 [757L] [2041L] [None] [] [] [] []
2009-09-17 03:48:52 submitted mmokrejs 637 EV71 ['EV71_1-748'] [None] end-to-end_full-length_mRNA 66967945 Human enterovirus 71 strain pinf7-54A from Taiwan, complete genome. natural_transcript 1 [1] [748] [] [] [] [] [] [] [] 2040 [757L] [2041L] [None] [] [] [] []
2009-09-17 02:20:30 changed mmokrejs 596 SV40 ['SV40_661-830'] [None] end-to-end_full-length_mRNA The SV40 late 19S long-spliced mRNA derived from transcription start site in genomic position 325. This is the most abundant variant (11% relative of all early and late mRNAs) of the 19S late transcripts. natural_transcript 4 [153] [322] [] [] [] [] [] [] [] 1895 [717L] [1896L] [None] [] [] [] []
2009-09-17 00:00:41 changed mmokrejs 337 IGF2 ['IGF2_leader2'] [None] both_UTRs_incomplete Homo sapiens insulin-like growth factor 2, leader 2 mRNA produced from P2 promoter (5.0 kb) in fetal tissues and transformed cells (possibly incomplete UTRs). natural_transcript 1 [200] [320] [] [] [] [] [] [] [] 1091 [486L] [1092L] [46L] [] [] [14L] []
2009-09-16 23:59:29 changed mmokrejs 337 IGF2_leader2 ['IGF2_leader2'] [None] both_UTRs_incomplete Homo sapiens insulin-like growth factor 2, leader 2 mRNA produced from P2 promoter (5.0 kb) in fetal tissues and transformed cells (possibly incomplete UTRs). natural_transcript 1 [200] [320] [] [] [] [] [] [] [] 1091 [486L] [1092L] [46L] [] [] [14L] []
2009-09-16 23:42:47 changed mmokrejs 337 IGF2 ['IGF2'] [None] both_UTRs_incomplete Homo sapiens insulin-like growth factor 2, leader 2 mRNA produced from P2 promoter (5.0 kb) in fetal tissues and transformed cells (possibly incomplete UTRs). natural_transcript 1 [200] [320] [] [] [] [] [] [] [] 1091 [486L] [1092L] [46L] [] [] [14L] []
2009-09-13 22:14:20 changed mmokrejs 40 CrPV ['CrPV_5NCR', 'CrPV_IGR'] [None, None] end-to-end_full-length_mRNA 21321708 Cricket paralysis virus (Dicistroviridae) nonstructural polyprotein and structural polyprotein genes, complete genome. natural_transcript 2 [1, 6025] [708, 6216] [] [] [] [] [] [] [] 101 [40L, 119L] [109L, 692L] [None, None] [] [] [44L] []
2009-09-13 21:50:58 changed mmokrejs 370 [] [] promoter-less_plasmid_without_putative_IRES 1 [] [] [] [] [] [] [] [] [] [] [] [] [] [] [] []
2009-09-05 13:13:08 changed mmokrejs 596 SV40 ['SV40_661-830'] [None] end-to-end_full-length_mRNA The SV40 late 19S long-spliced mRNA derived from transcription start site in genomic position 325. This is the most abundant variant (11% relative to all early and late mRNAs) of the 19S late transcripts. natural_transcript 4 [153] [322] [] [] [] [] [] [] [] 1895 [717L] [1896L] [None] [] [] [] []
2009-09-05 13:11:04 changed mmokrejs 577 FeLV-Notch2 ['FeLV-Notch2'] [None] 3UTR_incomplete Spliced subgenomic FeLV-Notch2 transcript with env gene replaced with part of Notch2 gene transcribed from the FeLV retroviral promoter. The transcript does not contain complete CDS region of the Notch2 protein but only the intracellular domain (ICD). natural_transcript 1 [582] [819] [] [] [] [] [] [] [] 1860 [701L] [1856L] [None] [] [] [] []
2009-09-05 13:07:58 changed mmokrejs 598 PV ['PV_type3_Leon'] [None] hopefully_full-length_mRNA 332895 Poliovirus P3/Leon/37 (type 3), complete genome. natural_transcript 1 [1] [742] [] [] [] [] [] [] [] 1910 [719L] [1912L] [None] [] [] [43L] []
2009-09-04 15:04:05 changed mmokrejs 25 ['c-myc_1-395'] ['functional'] both_UTRs_incomplete Full-length 5'-UTR (1-395 bp) of c-myc mRNA transcribed from major P2 promoter inserted between renilla and firefly luciferases as PvuII-NcoI insert plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [1178] [1571] ['in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo'] [None, None, None, None, None, None, None] ['100.000', '769.000', '0', '100.000', '100.000', '667.000', '165.000'] ['None', '100.000', 'None', 'None', 'None', '100.000', '100.000'] ['1.700', '10.000', '0', '1.400', '0', '11.000', '2.500'] [None, 'HRV-2', None, None, None, 'EMCV-R', 'EMCV-R'] ['endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_cytoplasmic_uncapped_T7_transcript_without_polyA_tail', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 1157 [25L] [1159L] [None] [41L, 418L, 419L, 427L, 516L, 520L, 525L] [] [] []
2009-09-04 15:01:31 changed mmokrejs 231 [] [] our_best_guess Putative in vitro derived T7 run-off transcript from BamHI linearized pRF plasmid. negative_control_plasmid_with_promoter_and_without_putative_IRES 2 [] [] [] [] [] [] [] [] [] 1136 [] [] [] [] [] [] []
2009-09-04 14:58:55 changed mmokrejs 184 [] [] 3UTR_incomplete Putative in vivo transcript from an empty vector pRF/pGL3R used as a negative control in many experiments. Although the plasmid sequence is known the exact location of the transcribed region is unknown. Our guess is based on the pDsRed2-C1 vector description from Clontech (Catalog #6974-1) and we conclude the major transcription start points could be at positions: 146, 184, 190, 195. The mRNA sequence used here starts from the most downstream putative transcription start site (5'-TATTCCAGAAGTAGTGA...) and continues to the end of the late SV40 poly(A) signal sequence (...AGTAAAACCTCTACAAATGTGGTA-3'). The chimeric intron in the transcribed region 276-408 is omitted from this mRNA sequence (information derived from GI:56089682). negative_control_plasmid_with_promoter_and_without_putative_IRES 2 [] [] [] [] [] [] [] [] [] 1134 [] [] [] [] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 595 ['rc_Apaf-1'] ['defective'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/rc_Apaf1/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human Apaf-1 5' UTR (nt from +3 to nt -577 of the original sequence) mRNA cloned between them in reverse complement orientation. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [3621] [4200] [] [] [] [] [] [] [] 1892 [716L] [1995L] [None] [] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 594 ['rc_DAP5'] ['defective'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/rc_DAP5/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the part of human DAP5 5' UTR (nt from -1 to nt -305 of the original sequence) mRNA cloned between them in reverse complement orientation. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [3621] [3925] [] [] [] [] [] [] [] 1889 [715L] [1891L] [None] [] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 636 [] [] hopefully_full-length_mRNA Bicistronic in vivo transcript mRNA with beta galactosidase and chloramphenicol acetyl transferase genes. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. negative_control_plasmid_with_promoter_and_without_putative_IRES 2 [] [] [] [] [] [] [] [] [] 2034 [] [] [] [] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 630 ['DAP5'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/DAP5/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human DAP5 5' UTR (nt from -305 to nt -1 of the original sequence) mRNA cloned between them. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3621] [3925] ['in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo'] [None, None, None, None, None, None] ['4.880', '2.300', '1.270', '1.000', '0.800', '0.070'] ['100.000', '100.000', '100.000', '100.000', '100.000', '100.000'] ['1.250', '1.000', '0.770', '0.350', '0.350', '0.290'] ['EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV'] ['endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 1990 [751L] [1845L] [None] [648L, 649L, 650L, 651L, 652L, 653L] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 631 ['Apaf-1'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/Apaf1/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human Apaf-1 5' UTR (nt from -577 to nt +3 of the original sequence) mRNA cloned between them. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3621] [4200] ['in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo'] [None, None, None, None, None, None] ['4.370', '2.100', '0.730', '0.230', '0.530', '0.070'] ['100.000', '100.000', '100.000', '100.000', '100.000', '100.000'] ['1.250', '1.000', '0.770', '0.350', '0.350', '0.290'] ['EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV'] ['endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 1997 [752L] [1999L] [None] [654L, 655L, 656L, 657L, 658L, 659L] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 593 ['BiP'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/BiP/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human BiP 5' UTR (nt from -222 to nt -1 of the original sequence) mRNA cloned between them. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3621] [3842] ['in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo'] [None, None, None, None, None, None] ['3.000', '3.000', '1.310', '0.310', '0.350', '0.490'] ['100.000', '100.000', '100.000', '100.000', '100.000', '100.000'] ['1.250', '1.000', '0.770', '0.350', '0.350', '0.290'] ['EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV'] ['endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 1886 [714L] [1888L] [None] [625L, 626L, 627L, 628L, 629L, 630L] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 592 ['rc_BiP'] ['defective'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/rc_BiP/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human BiP 5' UTR (nt from -1 to nt -222 of the original sequence) mRNA cloned between them in reverse complement orientation. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [3621] [3842] [] [] [] [] [] [] [] 1883 [713L] [1885L] [None] [] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 632 ['rc_XIAP'] ['defective'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/rc_XIAP/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human XIAP 5' UTR (nt from -1 to nt -993 of the original sequence) mRNA cloned between them in reverse complement orientation. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [3621] [4613] [] [] [] [] [] [] [] 2005 [753L] [2007L] [None] [] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 635 ['c-myc'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/myc/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the part of human c-myc 5' UTR (nt from -350 to nt +2 of the original sequence) mRNA cloned between them. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3621] [3972] ['in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo'] [None, None, None, None, None, None] ['3.060', '5.950', '3.230', '1.220', '0.350', '0.820'] ['100.000', '100.000', '100.000', '100.000', '100.000', '100.000'] ['1.250', '1.000', '0.770', '0.350', '0.350', '0.290'] ['EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV'] ['endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 2028 [756L] [2030L] [None] [667L, 668L, 669L, 670L, 671L, 672L] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 634 ['rc_c-myc'] ['defective'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/rc_myc/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the part of human c-myc 5' UTR (nt from +2 to nt -350 of the original sequence) mRNA cloned between them in reverse complement orientation. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_without_translational_characterization 2 [3621] [3972] [] [] [] [] [] [] [] 2028 [755L] [2030L] [None] [] [] [] []
2009-09-04 12:19:56 reviewed mmokrejs 244 ['XIAP'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/XIAP/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human XIAP 5' UTR (nt from -993 to nt -1 of the original sequence) mRNA cloned between them. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3621] [4613] ['in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo'] [None, None, None, None, None, None, None, None, None] ['100.000', '100.000', '37.500', '22.000', '12.100', '6.610', '11.000', '1.130', '100.000'] ['None', 'None', '100.000', '100.000', '100.000', '100.000', '100.000', '100.000', 'None'] ['0.019', '1.140', '1.250', '1.000', '0.770', '0.350', '0.350', '0.290', '0.880'] [None, None, 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', None] ['exogenous_RNA_with_GpppG_cap_with_polyA_tail', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 2004 [394L] [828L] [None] [299L, 660L, 661L, 662L, 663L, 664L, 665L, 666L, 673L] [] [] []
2009-09-03 20:25:57 changed vasek 244 ['XIAP'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/XIAP/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human XIAP 5' UTR (nt from -993 to nt -1 of the original sequence) mRNA cloned between them. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3621] [4613] ['in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo'] [None, None, None, None, None, None, None, None, None] ['100.000', '100.000', '37.500', '22.000', '12.100', '6.610', '11.000', '1.130', '100'] ['None', 'None', '100.000', '100.000', '100.000', '100.000', '100.000', '100.000', 'None'] ['0.019', '1.140', '1.250', '1.000', '0.770', '0.350', '0.350', '0.290', '0.88'] [None, None, 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', None] ['exogenous_RNA_with_GpppG_cap_with_polyA_tail', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 2004 [394L] [828L] [None] [299L, 660L, 661L, 662L, 663L, 664L, 665L, 666L, 673L] [] [] []
2009-09-03 20:03:16 changed vasek 472 ['XIAP'] ['functional'] promoter-less_plasmid_with_putative_IRES 2 [3896] [4060] [] [] [] [] [] [] [] [606L] [2039L] [None] [] [] [] []
2009-09-03 19:21:43 changed vasek 486 ['XIAP'] ['functional'] 3UTR_possibly_incomplete Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/5'(-162)/CAT which comprises beta galactosidase and chloramphenicol acetyl transferase as the first and the second cistron, respectively and the part of human XIAP mRNA (from nt -162 to nt -1 of the original sequence) mRNA. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3621] [3782] ['in vivo', 'in vivo'] [None, None] ['100.000', '100.000'] ['None', 'None'] ['1.000', '0.300'] [None, None] ['endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 1592 [620L] [1543L] [38L] [537L, 538L] [] [23L] []
2009-09-03 19:20:56 changed vasek 487 ['ELG1'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pBiCELG1 which comprises beta galactosidase and chloramphenicol acetyl transferase as the first and the second cistron, respectively and the part (from nt -803 to nt -461 of the original sequence)of 5' UTR from human ELG1 mRNA. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3626] [4085] ['in vivo'] [None] ['7.300'] ['100.000'] ['0.300'] ['XIAP'] ['endogenous_nuclear_RNA_Pol_II_transcript'] 1594 [621L] [1545L] [None] [539L] [] [] []
2009-09-03 19:20:08 changed vasek 488 ['AQP4'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pBiCAQP4 which comprises beta galactosidase and chloramphenicol acetyl transferase as the first and the second cistron, respectively and almost the full length 5' UTR from human AQP4 mRNA (from nt -275 to nt -1 of the original sequence) and the first 7 nts (+1 to +7) from AQP4 ORF. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3626] [3911] ['in vivo'] [None] ['2.000'] ['100.000'] ['0.300'] ['XIAP'] ['endogenous_nuclear_RNA_Pol_II_transcript'] 1596 [622L] [1551L] [None] [540L] [] [] []
2009-09-03 19:15:30 changed vasek 244 ['XIAP'] ['functional'] hopefully_full-length_mRNA Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/XIAP/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the first and the second cistron respectively and the whole part of human XIAP 5' UTR (nt from -993 to nt -1 of the original sequence) mRNA cloned between them. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. plasmid_with_promoter_and_putative_IRES_translationally_characterized 2 [3621] [4613] ['in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo', 'in vivo'] [None, None, None, None, None, None, None, None, None] ['100.000', '100.000', '37.500', '22.000', '12.100', '6.610', '11.000', '1.130', '150.000'] ['None', 'None', '100.000', '100.000', '100.000', '100.000', '100.000', '100.000', 'None'] ['0.019', '1.140', '1.250', '1.000', '0.770', '0.350', '0.350', '0.290', '1.000'] [None, None, 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', 'EMCV', None] ['exogenous_RNA_with_GpppG_cap_with_polyA_tail', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript', 'endogenous_nuclear_RNA_Pol_II_transcript'] 2004 [394L] [828L] [None] [299L, 660L, 661L, 662L, 663L, 664L, 665L, 666L, 673L] [] [] []
2009-09-03 19:11:16 changed vasek 237 [] [] hopefully_full-length_mRNA Bicistronic in vivo transcript mRNA with beta galactosidase and chloramphenicol acetyl transferase genes. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. negative_control_plasmid_with_promoter_and_without_putative_IRES 2 [] [] [] [] [] [] [] [] [] 2031 [] [] [] [] [] [] []
2009-09-03 19:01:37 submitted vasek 636 [] [] hopefully_full-length_mRNA Bicistronic in vivo transcript mRNA with beta galactosidase and chloramphenicol acetyl transferase genes. The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be slightly wrong. negative_control_plasmid_with_promoter_and_without_putative_IRES 2 [] [] [] [] [] [] [] [] [] 2034 [] [] [] [] [] [] []
2009-09-03 14:55:13 changed mmokrejs 35 c-myc ['c-myc'] [None] 3UTR_incomplete Homo sapiens cDNA of c-myc proto-oncogene. Poly(A) tail dropped. natural_transcript 2 [1] [393] [] [] [] [] [] [] [] 1993 [35L] [1131L] [20L] [] [2L, 8L, 9L, 10L, 11L, 12L] [1L] []
2009-09-03 14:14:10 changed mmokrejs 109 XIAP ['XIAP_5-464', 'XIAP_305-466'] [None, None] nonexisting_chimera_of_GenBank_record_and_tested_IRES_fragment Assembled chimeric sequence created by utilization of the 5'-UTR from GI:28290426 with the remaining sequence from GI:32528298 natural_transcript 1 [5, 305] [464, 466] [] [] [] [] [] [] [] 681 [116L, 589L] [281L, 1543L] [None, 38L] [] [] [15L] []
2009-09-03 14:00:17 changed mmokrejs 35 c-myc ['c-myc'] [None] 3UTR_incomplete Homo sapiens cDNA of c-myc proto-oncogene. Poly(A) tail dropped. natural_transcript 2 [1] [393] [] [] [] [] [] [] [] 1993 [35L] [1131L] [20L] [] [2L, 8L, 9L, 10L, 11L, 12L] [1L] []
2009-09-03 13:54:41 changed mmokrejs 571 VEGF-A ['VEGF-A_-133_-1'] [None] our_best_guess 160358806 Mus musculus vascular endothelial growth factor A (Vegfa), transcript variant 3, mRNA. Poly(A)-tail sequence was dropped. natural_transcript 2 [889] [1021] [] [] [] [] [] [] [] 2018 [696L] [1834L] [None] [] [] [] []
2009-09-03 13:50:39 changed mmokrejs 109 XIAP ['XIAP_5-464', 'XIAP_305-466'] [None, None] nonexisting_chimera_of_GenBank_record_and_tested_IRES_fragment Assembled chimeric sequence created by utilization of the 5'-UTR from GI:28290426 "BX119811 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGp998G014639 ; IMAGE:1893144" with the remaining sequence from Refseq sequence GI:32528298. natural_transcript 1 [5, 305] [464, 466] [] [] [] [] [] [] [] 681 [116L, 589L] [281L, 1543L] [None, 38L] [] [] [15L] []
2009-09-03 13:45:39 changed mmokrejs 109 XIAP ['XIAP_5-464', 'XIAP_305-466'] [None, None] nonexisting_chimera_of_GenBank_record_and_tested_IRES_fragment Assembled chimeric sequence created by utilization of the 5'-UTR from GI:28290426 "BX119811 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGp998G014639 ; IMAGE:1893144" with the remaining sequence from Refseq sequence GI:32528298. natural_transcript 1 [5, 305] [464, 466] [] [] [] [] [] [] [] 681 [116L, 589L] [281L, 1543L] [None, 38L] [] [] [15L] []
2009-09-03 13:40:02 changed mmokrejs 571 VEGF-A ['VEGF-A_-133_-1'] [None] our_best_guess 160358806 Mus musculus vascular endothelial growth factor A (Vegfa), transcript variant 3, mRNA. Poly(A)-tail sequence was dropped. natural_transcript 2 [889] [1021] [] [] [] [] [] [] [] 2018 [696L] [1834L] [None] [] [] [] []
2009-09-03 13:38:32 changed mmokrejs 1 Hif1a ['HIF1a'] [None] our_best_guess 7363432 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), mRNA. Poly(A) tail dropped. natural_transcript 1 [1] [257] [] [] [] [] [] [] [] 1992 [1L] [2L] [None] [] [] [] []
2009-09-03 13:37:27 changed mmokrejs 35 c-myc ['c-myc'] [None] 3UTR_incomplete Homo sapiens cDNA of c-myc proto-oncogene. Poly(A) tail dropped. natural_transcript 2 [1] [393] [] [] [] [] [] [] [] 1993 [35L] [1131L] [20L] [] [2L, 8L, 9L, 10L, 11L, 12L] [1L] []
2009-09-03 13:33:06 changed mmokrejs 1 Hif1a ['HIF1a'] [None] our_best_guess 7363432 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), mRNA. Poly(A) tail dropped. natural_transcript 1 [1] [257] [] [] [] [] [] [] [] 1992 [1L] [2L] [None] [] [] [] []
2009-09-03 13:29:04 changed mmokrejs 140 EMCV-R ['EMCV-R'] [None] end-to-end_full-length_mRNA Encephalomyocarditis virus, complete genome natural_transcript 1 [257] [832] [] [] [] [] [] [] [] 684 [156L] [685L] [None] [] [] [40L] []
2009-09-03 13:15:01 changed mmokrejs 1 Hif1a ['HIF1a'] [None] our_best_guess 7363432 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), mRNA. Poly(A) tail dropped. natural_transcript 1 [1] [257] [] [] [] [] [] [] [] 1992 [1L] [2L] [None] [] [] [] []
2009-09-03 13:12:41 changed mmokrejs 140 EMCV-R ['EMCV-R'] [None] end-to-end_full-length_mRNA Encephalomyocarditis virus, complete genome natural_transcript 1 [257] [832] [] [] [] [] [] [] [] 684 [156L] [685L] [None] [] [] [40L] []
2009-09-03 13:05:58 changed mmokrejs 571 VEGF-A ['VEGF-A_-133_-1'] [None] our_best_guess 160358806 Mus musculus vascular endothelial growth factor A (Vegfa), transcript variant 3, mRNA. Poly(A)-tail sequence was dropped. natural_transcript 2 [889] [1021] [] [] [] [] [] [] [] 2018 [696L] [1834L] [None] [] [] [] []
2009-09-03 12:47:06 changed mmokrejs 570 BiP ['BiP_-222_-3'] [None] hopefully_full-length_mRNA 1143491 H.sapiens mRNA for BiP protein mRNA. Poly(A)-tail sequence was dropped. natural_transcript 1 [1] [222] [] [] [] [] [] [] [] 2017 [693L] [1816L] [None] [] [] [] []
2009-09-03 12:33:11 changed mmokrejs 1 Hif1a ['HIF1a'] [None] our_best_guess 7363432 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), mRNA. Poly(A) tail dropped. natural_transcript 1 [1] [257] [] [] [] [] [] [] [] 1992 [1L] [2L] [None] [] [] [] []
Last change to the database: 2019-03-18 09:32:49 GMT+1