A promoter reported in cDNA corresponding to IRES sequence: yes
The total number of notable open-reading frames (ORFs): 2
Summary of possible issues when IRES cDNA is experimentally transcribed in vivo:
Summary of experiments studying integrity of the in vivo transcripts in a particular host:
Integrity (uniformity) of mRNA tested using Northern-blot: not_tested
Integrity (uniformity) of mRNA tested using RNase protection: not_tested
Integrity (uniformity) of mRNA tested using 5'-RACE: not_tested
Integrity (uniformity) of mRNA tested using primer extension : not_tested
Integrity (uniformity) of mRNA tested using RT-PCR: not_tested
Integrity (uniformity) of mRNA tested using real-time quantitative polymerase chain reaction (rtqPCR): not_tested
Integrity (uniformity) of mRNA tested using RNAi: not_tested
Integrity (uniformity) of mRNA tested using S1 nuclease mapping: not_tested
Cryptic promoter presence was confirmed by expression from a promoter-less plasmid: weak_promoter_confirmed
Cryptic promoter presence was confirmed in an experimental setup involving inducible promoter: not_tested
Integrity (uniformity) of mRNA molecules or possible promoter presence expressed in vivo was tested using another method, please specify in Remarks: not_tested
The abbreviated name of this ORF/gene: polyprotein
The description of the protein encoded in this ORF: structural polyprotein
The translational frameshift (ribosome slippage) involved: 0
The ribosome read-through involved: no
The alternative forms of this protein occur by the alternative initiation of translation: not tested
The ORF absolute position (the base range includes START and STOP codons or their equivalents): 7107-9560
Remarks:
The second ORF is not initiated by a +1 frameshift as proposed originally but the first two aminoacid residues
come from 'CCTGCA' in Domier et al. (2000), Figure 6.
The cryptic promoter activity in Spodoptera frugiperda Sf21 cells was shown by Wu et al. (2008), and
postulated already in Wu et al. (2007).
Please refer also to patent no. WO 02/12522.
The IRES absolute position (the range includes START and STOP codons or their equivalents): 1-579
Conclusion: putative_IRES
How IRES boundaries were determined: experimentally_determined
The sequence of IRES region aligned to its secondary structure (if available):
Remarks:
Sequence 1 from Patent WO0212522. The IRES is also functional in wheat-germ lysates (Woolaway et al., (2001),
Figure 2), also in Drosophila SL2 cell extracts (Woolaway et al., (2001), Figure 3), in Spodoptera frugiperda
Sf21 cells (Royall et al., (2004)). In contrast, EMCV IRES is functional only in mammalian cells or cell
extracts.
Robert and Groppeli (2009) reported that the 5'-UTR IRES is largely unstructured.
Wu et al. (2007) reported that the 5NTR IRES was functional in in baculovirus-infected Sf21 cells.
The IRES absolute position (the range includes START and STOP codons or their equivalents): 6875-7106
Conclusion: strongly_supported_IRES
How IRES boundaries were determined: experimentally_determined
5'-end of IRES relative to last base of the STOP codon of the upstream ORF: 299
3'-end of IRES relative to last base of the STOP codon of the upstream ORF: 530
5'-end of IRES relative to first base of the START codon of the downstream ORF: -232
3'-end of IRES relative to first base of the START codon of the downstream ORF: -1
The sequence of IRES region aligned to its secondary structure (if available):
Remarks:
The IRES is followed by CCU translation initiation codon. Localization of RhPV_IGR IRES containing RNA in
80S ribosomal fraction was shown in Domier et al. (2003), Figure 3.
ITAF description (long): eIF4G is the scaffolding protein bridging together the cap-binding protein eIF4E with other proteins of the
initiating ribosome
3.5.2. Organisms or in vitro systems where this ITAF was functionally studied:
Organism or in vitro system where ITAF was shown:
Necessity of ITAF for translation in this particular organism or system: stimulatory
Method used to demonstrate ITAF effect: in_vitro
In vitro system used to demonstrate ITAF effect: other