IRESite_Id:522 characteristics of c-IAP1_-150/-1 IRES studied in hp-pbetaGAL/HIAP2(1073-1222)/CAT plasmid

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `522`	Version: `4`
Originaly submitted by: Václav Vopálenský
Reviewed by: Martin Mokrejš	Last change: `2008-10-27 23:32:39`

IRESite record type:

plasmid_with_promoter_and_putative_IRES_translationally_characterized

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
3UTR_possibly_incomplete

The mRNA/+RNA description:

Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid
hp-pbetaGAL/HIAP2(1073-1222)/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as
the first and the second cistron respectively and the part of human c-IAP1 5' UTR (nt from -150 to nt -1 of
the original sequence) mRNA mRNA cloned between them, with a hairpin in front of the first cistron.
The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be
slightly wrong.

The mRNA/+RNA sequence represented in the +DNA notation:

agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc ccggccggcc ggccggccgg ccaaaggccg 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

gccggccggc cggccggcta gcggtacggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgca gatatccatc acactggcgg 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

ccgcaattcc cggggatcga aagagcctgc taaagcaaaa aagaagtcac catgtcgttt actttgacca acaagaacgt gattttcgtt gccggtctgg 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gaggcattgg tctggacacc agcaaggagc tgctcaagcg cgatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

ttggtggcga cgactcctgg agcccgtcag tatcggcgga attacagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa aaacacaaac ttttggatgt tcggtttatt ctttttcttt 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

tactttttta tcatgggagc ctacttcccg tttttcccga tttggctaca tgacatcaac catatcagca aaagtgatac gggtattatt tttgccgcta 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

tttctctgtt ctcgctatta ttccaaccgc tgtttggtct gctttctgac aaactcggcc tcgactctag gcggccgcct cgagtgattt ctttttgtgg 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

taaaaatctt agttcatgtg aagaaatttc atgtgaatgt tttagctatc aaacagtact gtcacctact catacacaaa actgcctccc aaagactttt 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

cccaggtccc tcgtatcaaa acattaagag tatactcgag atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgccgg atctttgtga 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

tttgtccaaa ctcatcaatg tatcttatca tgtctgccta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581

Credibility of mRNA sequence:

reverse_engineered_sequence_and_should_match_experiment_except_3UTR

The name of the plasmid:
hp-pbetaGAL/HIAP2(1073-1222)/CAT The name of the promoter used to express this mRNA:
CMV

Description of the plasmid (facultative for promoter-less plasmid records):
Plasmid containing part of human c-IAP1 5' UTR (nt from -150 to nt -1 of the original sequence) inserted between beta galactosidase and chloramphenicol acetyltransferase reporter genes. A synthetic DNA fragment was inserted into the bicistronic constructs to introduce a stable stem loop structure 72 nt downstream from CMV transcription start site.

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

A promoter reported in cDNA corresponding to IRES sequence:

not tested

The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
c-IAP1

The origin of IRES in the plasmid:

nuclear

The donor organism of the IRES segment:

Homo sapiens Jurkat

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

ccgatcccct atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta ccccggccgg ccggccggcc 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

ggccaaaggc cggccggccg gccggccggc tagcggtacg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattctg cagatatcca 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

tcacactggc ggccgcaatt cccggggatc gaaagagcct gctaaagcaa aaaagaagtc accatgtcgt ttactttgac caacaagaac gtgattttcg 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

ttgccggtct gggaggcatt ggtctggaca ccagcaagga gctgctcaag cgcgatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

gttgcagtgc acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattacagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

aaaataataa taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa acttttggat gttcggttta 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

ttctttttct tttacttttt tatcatggga gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat acgggtatta 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg acaaactcgg cctcgactct aggcggccgc ctcgagtgat 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

ttctttttgt ggtaaaaatc ttagttcatg tgaagaaatt tcatgtgaat gttttagcta tcaaacagta ctgtcaccta ctcatacaca aaactgcctc 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

ccaaagactt ttcccaggtc cctcgtatca aaacattaag agtatactcg agatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgcc 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgcc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 
^8001      ^8011      ^8021      ^8031      ^8041      ^8051      ^8061      ^8071      ^8081      ^8091      

tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 
^8101      ^8111      ^8121      ^8131      ^8141      ^8151      ^8161      ^8171      ^8181      ^8191      

gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 
^8201      ^8211      ^8221      ^8231      ^8241      ^8251      ^8261      ^8271      ^8281      ^8291      

agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg 
^8301      ^8311      ^8321      ^8331      ^8341      ^8351      ^8361      ^8371      ^8381      ^8391      

ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 
^8401      ^8411      ^8421      ^8431      ^8441      ^8451      ^8461      ^8471      ^8481      ^8491      

cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
^8501      ^8511      ^8521      ^8531      ^8541      ^8551      ^8561      ^8571      ^8581      ^8591      

atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
^8601      ^8611      ^8621      ^8631      ^8641      ^8651      ^8661      ^8671      ^8681      ^8691      

cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
^8701      ^8711      ^8721      ^8731      ^8741      ^8751      ^8761      ^8771      ^8781      ^8791      

cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
^8801      ^8811      ^8821      ^8831      ^8841      ^8851      ^8861      ^8871      ^8881      ^8891      

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
^8901      ^8911      ^8921      ^8931      ^8941      ^8951      ^8961      ^8971      ^8981      ^8991      

tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
^9001      ^9011      ^9021      ^9031      ^9041      ^9051      ^9061      ^9071      ^9081      ^9091      

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
^9101      ^9111      ^9121      ^9131      ^9141      ^9151      ^9161      ^9171      ^9181      ^9191      

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
^9201      ^9211      ^9221      ^9231      ^9241      ^9251      ^9261      ^9271      ^9281      ^9291      

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
^9301      ^9311      ^9321      ^9331      ^9341      ^9351      ^9361      ^9371      ^9381      ^9391      

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
^9401      ^9411      ^9421      ^9431      ^9441      ^9451      ^9461      ^9471      ^9481      ^9491      

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
^9501      ^9511      ^9521      ^9531      ^9541      ^9551      ^9561      ^9571      ^9581      ^9591      

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
^9601      ^9611      ^9621      ^9631      ^9641      ^9651      ^9661      ^9671      ^9681      ^9691      

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
^9701      ^9711      ^9721      ^9731      ^9741      ^9751      ^9761      ^9771      ^9781      ^9791      

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
^9801      ^9811      ^9821      ^9831      ^9841      ^9851      ^9861      ^9871      ^9881      ^9891      

ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
^9901      ^9911      ^9921      ^9931      ^9941      ^9951      ^9961      ^9971      ^9981      ^9991      

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
^10001     ^10011     ^10021     ^10031     ^10041     ^10051     ^10061     ^10071     ^10081     ^10091     

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
^10101     ^10111     ^10121     ^10131     ^10141     ^10151     ^10161     ^10171     ^10181     ^10191     

cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
^10201     ^10211     ^10221     ^10231     ^10241     ^10251     ^10261     ^10271     ^10281     ^10291     

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
^10301     ^10311     ^10321     ^10331     ^10341     ^10351     ^10361     ^10371     ^10381     ^10391     

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
^10401     ^10411     ^10421     ^10431     ^10441     ^10451     ^10461     ^10471     ^10481     ^10491     

cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
^10501     ^10511     ^10521     ^10531     ^10541     ^10551     ^10561     ^10571     ^10581     ^10591     

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
^10601     ^10611     ^10621     ^10631     ^10641     ^10651     ^10661     ^10671     ^10681     ^10691     

aagcatttat cagggttatt gtctcatgag
^10701     ^10711     ^10721

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

The total number of notable open-reading frames (ORFs):

2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
LacZ

The description of the protein encoded in this ORF:
beta galactosidase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

252-3395

ORF

ORF position:

2

Version: `1`	Last change: `2008-10-27 23:32:39`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
CAT

The description of the protein encoded in this ORF:
chloramphenicol acetyltransferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

3841-4392

Citations:

Warnakulasuriyarachchi D., Cerquozzi S., Cheung H. H., Holcik M. (2004) Translational induction of the inhibitor of apoptosis protein HIAP2 during endoplasmic reticulum stress attenuates cell death and is mediated via an inducible internal ribosome entry site element. J. Biol. Chem. 279(17):17148-17157

IRESs:

IRES:

Version: `2`	Last change: `2008-10-23 13:13:42`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš
The IRES name: `c-IAP1_-150/-1`	The functional status of IRES: `functional`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

3685-3834

How IRES boundaries were determined:
experimentally_determined

5'-end of IRES relative to last base of the STOP codon of the upstream ORF: `290`	3'-end of IRES relative to last base of the STOP codon of the upstream ORF: `439`
5'-end of IRES relative to first base of the START codon of the downstream ORF: `-156`	3'-end of IRES relative to first base of the START codon of the downstream ORF: `-7`

The sequence of IRES region aligned to its secondary structure (if available):

tgatttcttt ttgtggtaaa aatcttagtt catgtgaaga aatttcatgt gaatgtttta gctatcaaac agtactgtca cctactcata cacaaaactg 
^3685      ^3695      ^3705      ^3715      ^3725      ^3735      ^3745      ^3755      ^3765      ^3775      

cctcccaaag acttttccca ggtccctcgt atcaaaacat taagagtata
^3785      ^3795      ^3805      ^3815      ^3825

Remarks:

c-IAP1 IRES represents part of the human c-IAP1 5' UTR (nt from -150 to nt -1).

Citations:

The translation experiments:

Translation results:

IRESite Translation Id: 572

Version: `5`	Last change: `2008-10-23 13:13:42`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The translation method used to study IRES function:
in vivo

The organism used for translation:

Homo sapiens HEK 293T (ATCC 293tsA1609neo)

The temperature (in degrees of Celsia):
37

The relative translation efficiency in % of this IRES:

100.000

	Name of the plasmid used as the negative control. `hp-pbetaGAL/CAT`
	`IRESite Id` of the plasmid used as negative control. `521`
	The relative translation efficiency in % of the negative control: `13.100`
	The size (length) of intercistronic region in the negative control: `337`

The effect of 5'-cap analogs on translation:
not tested

Rapamycin affects translation:
not tested

Type of RNA subject to translation:

endogenous_nuclear_RNA_Pol_II_transcript

Remarks:

Translational efficiency was determined in thapsigargin (resuspended in DMSO)-treated cells.
DMSO-alone treated cells have about 10 times higher CAT production from hp-pbetaGAL/HIAP2/CAT plasmid compared
with empty hp-pbetaGAL/CAT vector.
Translational data are derived from Fig. 2C.

When rabbit reticulocyte lysates were primed with the hairpin-containing c-IAP1 IRES reporter construct
(hp-pbetaGAL/HIAP2(1073-1222)/CAT) and either the FLAGp97- or FLAG-p86-expressing plasmids, the addition of
p86 fragment resulted in the activation of c-IAP1 IRES (approximately 900 times higher production of CAT
protein, compared with the bicistronic construct that lacks the 5' UTR insert (hp-pbetaGAL/CAT)). See Figure
4D.

Citations:

Last change to the database: 2019-03-18 09:32:49 GMT+1