IRESite_Id:526 characteristics of c-IAP1_-603/-1 IRES studied in pbetaGAL/HIAP2(620-1222)/CAT plasmid

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `526`	Version: `4`
Originaly submitted by: Václav Vopálenský
Reviewed by: Martin Mokrejš	Last change: `2008-10-27 23:40:20`

IRESite record type:

plasmid_with_promoter_and_putative_IRES_translationally_characterized

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
3UTR_possibly_incomplete

The mRNA/+RNA description:

Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid
pbetaGAL/HIAP2(620-1222)/CAT which comprises beta galactosidase and chloramphenicol acetyltransferase as the
first and the second cistron respectively and the part of human c-IAP1 5' UTR (nt from -603 to nt -1 of the
original sequence) mRNA cloned between them.
The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be
slightly wrong.

The mRNA/+RNA sequence represented in the +DNA notation:

agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc gagctcggat ccactagtaa cggccgccag 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

tgtgctggaa ttctgcagat atccatcaca ctggcggccg caattcccgg ggatcgaaag agcctgctaa agcaaaaaag aagtcaccat gtcgtttact 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

ttgaccaaca agaacgtgat tttcgttgcc ggtctgggag gcattggtct ggacaccagc aaggagctgc tcaagcgcga tcccgtcgtt ttacaacgtc 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt acagctgagc gccggtcgct 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttaaaaaa 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

cacaaacttt tggatgttcg gtttattctt tttcttttac ttttttatca tgggagccta cttcccgttt ttcccgattt ggctacatga catcaaccat 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc caaccgctgt ttggtctgct ttctgacaaa ctcggcctcg 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

actctaggcg gccgcctcga gaattacttt tgtttattgg aataaaatga gattttttgg gttgtcatgt taaagtgctt atagggaaag aagcctgcat 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

ataatttttt accttgtggc ataatcagta attggtctgt tattcaggct tcatagcttg taaccaaata taaataaaag gcataattta ggtattctat 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

agttgcttag aattttgtta atataaatct ctgtgaaaaa tcaaggagtt ttaatatttt cagaagtgca tccacctttc agggctttaa gttagtatta 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

ctcaagatta tgaacaaata gcacttaggt tacctgaaag agttactaca accccaaaga gttgtgttct aagtagtatc ttggtaattc agagagatac 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

tcatcctacc tgaatataaa ctgagataaa tccagtaaag aaagtgtagt aaattctaca taagagtcta tcattgattt ctttttgtgg taaaaatctt 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

agttcatgtg aagaaatttc atgtgaatgt tttagctatc aaacagtact gtcacctact catgcacaaa actgcctccc aaagactttt cccaggtccc 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

tcgtatcaaa acattaagag tatactcgag atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgccgg atctttgtga aggaacctta 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

ctcatcaatg tatcttatca tgtctgccta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971

Credibility of mRNA sequence:

reverse_engineered_sequence_and_should_match_experiment_except_3UTR

The name of the plasmid:
pbetaGAL/HIAP2(620-1222)/CAT The name of the promoter used to express this mRNA:
CMV

Description of the plasmid (facultative for promoter-less plasmid records):
Plasmid containing part of human c-IAP1 5' UTR (nt from -603 to nt -1 of the original sequence) inserted between beta galactosidase and chloramphenicol acetyltransferase reporter genes.

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

A promoter reported in cDNA corresponding to IRES sequence:

not tested

The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
c-IAP1

The origin of IRES in the plasmid:

nuclear

The donor organism of the IRES segment:

Homo sapiens Jurkat

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

ccgatcccct atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta ccgagctcgg atccactagt 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

aacggccgcc agtgtgctgg aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct aaagcaaaaa agaagtcacc 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

atgtcgttta ctttgaccaa caagaacgtg attttcgttg ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

ctatttaaaa aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt ttttcccgat ttggctacat 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

gacatcaacc atatcagcaa aagtgatacg ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

aactcggcct cgactctagg cggccgcctc gagaattact tttgtttatt ggaataaaat gagatttttt gggttgtcat gttaaagtgc ttatagggaa 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

agaagcctgc atataatttt ttaccttgtg gcataatcag taattggtct gttattcagg cttcatagct tgtaaccaaa tataaataaa aggcataatt 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

taggtattct atagttgctt agaattttgt taatataaat ctctgtgaaa aatcaaggag ttttaatatt ttcagaagtg catccacctt tcagggcttt 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

aagttagtat tactcaagat tatgaacaaa tagcacttag gttacctgaa agagttacta caaccccaaa gagttgtgtt ctaagtagta tcttggtaat 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

tcagagagat actcatccta cctgaatata aactgagata aatccagtaa agaaagtgta gtaaattcta cataagagtc tatcattgat ttctttttgt 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

ggtaaaaatc ttagttcatg tgaagaaatt tcatgtgaat gttttagcta tcaaacagta ctgtcaccta ctcatgcaca aaactgcctc ccaaagactt 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

ttcccaggtc cctcgtatca aaacattaag agtatactcg agatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgcc ggatctttgt 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

ggtttgtcca aactcatcaa tgtatcttat catgtctgcc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 
^8001      ^8011      ^8021      ^8031      ^8041      ^8051      ^8061      ^8071      ^8081      ^8091      

gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 
^8101      ^8111      ^8121      ^8131      ^8141      ^8151      ^8161      ^8171      ^8181      ^8191      

gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 
^8201      ^8211      ^8221      ^8231      ^8241      ^8251      ^8261      ^8271      ^8281      ^8291      

cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
^8301      ^8311      ^8321      ^8331      ^8341      ^8351      ^8361      ^8371      ^8381      ^8391      

cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 
^8401      ^8411      ^8421      ^8431      ^8441      ^8451      ^8461      ^8471      ^8481      ^8491      

gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 
^8501      ^8511      ^8521      ^8531      ^8541      ^8551      ^8561      ^8571      ^8581      ^8591      

ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 
^8601      ^8611      ^8621      ^8631      ^8641      ^8651      ^8661      ^8671      ^8681      ^8691      

agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 
^8701      ^8711      ^8721      ^8731      ^8741      ^8751      ^8761      ^8771      ^8781      ^8791      

gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 
^8801      ^8811      ^8821      ^8831      ^8841      ^8851      ^8861      ^8871      ^8881      ^8891      

ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 
^8901      ^8911      ^8921      ^8931      ^8941      ^8951      ^8961      ^8971      ^8981      ^8991      

gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 
^9001      ^9011      ^9021      ^9031      ^9041      ^9051      ^9061      ^9071      ^9081      ^9091      

gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 
^9101      ^9111      ^9121      ^9131      ^9141      ^9151      ^9161      ^9171      ^9181      ^9191      

gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
^9201      ^9211      ^9221      ^9231      ^9241      ^9251      ^9261      ^9271      ^9281      ^9291      

gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
^9301      ^9311      ^9321      ^9331      ^9341      ^9351      ^9361      ^9371      ^9381      ^9391      

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
^9401      ^9411      ^9421      ^9431      ^9441      ^9451      ^9461      ^9471      ^9481      ^9491      

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
^9501      ^9511      ^9521      ^9531      ^9541      ^9551      ^9561      ^9571      ^9581      ^9591      

ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
^9601      ^9611      ^9621      ^9631      ^9641      ^9651      ^9661      ^9671      ^9681      ^9691      

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
^9701      ^9711      ^9721      ^9731      ^9741      ^9751      ^9761      ^9771      ^9781      ^9791      

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
^9801      ^9811      ^9821      ^9831      ^9841      ^9851      ^9861      ^9871      ^9881      ^9891      

atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
^9901      ^9911      ^9921      ^9931      ^9941      ^9951      ^9961      ^9971      ^9981      ^9991      

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
^10001     ^10011     ^10021     ^10031     ^10041     ^10051     ^10061     ^10071     ^10081     ^10091     

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
^10101     ^10111     ^10121     ^10131     ^10141     ^10151     ^10161     ^10171     ^10181     ^10191     

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
^10201     ^10211     ^10221     ^10231     ^10241     ^10251     ^10261     ^10271     ^10281     ^10291     

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
^10301     ^10311     ^10321     ^10331     ^10341     ^10351     ^10361     ^10371     ^10381     ^10391     

ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
^10401     ^10411     ^10421     ^10431     ^10441     ^10451     ^10461     ^10471     ^10481     ^10491     

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
^10501     ^10511     ^10521     ^10531     ^10541     ^10551     ^10561     ^10571     ^10581     ^10591     

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
^10601     ^10611     ^10621     ^10631     ^10641     ^10651     ^10661     ^10671     ^10681     ^10691     

actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
^10701     ^10711     ^10721     ^10731     ^10741     ^10751     ^10761     ^10771     ^10781     ^10791     

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
^10801     ^10811     ^10821     ^10831     ^10841     ^10851     ^10861     ^10871     ^10881     ^10891     

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
^10901     ^10911     ^10921     ^10931     ^10941     ^10951     ^10961     ^10971     ^10981     ^10991     

aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
^11001     ^11011     ^11021     ^11031     ^11041     ^11051     ^11061     ^11071     ^11081     ^11091     

cagggttatt gtctcatgag
^11101     ^11111

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

The total number of notable open-reading frames (ORFs):

2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
LacZ

The description of the protein encoded in this ORF:
beta galactosidase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

189-3332

ORF

ORF position:

2

Version: `1`	Last change: `2008-10-27 23:40:20`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
CAT

The description of the protein encoded in this ORF:
chloramphenicol acetyltransferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

4231-4890

Citations:

Warnakulasuriyarachchi D., Cerquozzi S., Cheung H. H., Holcik M. (2004) Translational induction of the inhibitor of apoptosis protein HIAP2 during endoplasmic reticulum stress attenuates cell death and is mediated via an inducible internal ribosome entry site element. J. Biol. Chem. 279(17):17148-17157

IRESs:

IRES:

Version: `1`	Last change: `2008-10-23 13:18:45`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš
The IRES name: `c-IAP1_-603/-1`	The functional status of IRES: `functional`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

3622-4224

How IRES boundaries were determined:
experimentally_determined

5'-end of IRES relative to last base of the STOP codon of the upstream ORF: `290`	3'-end of IRES relative to last base of the STOP codon of the upstream ORF: `892`
5'-end of IRES relative to first base of the START codon of the downstream ORF: `-609`	3'-end of IRES relative to first base of the START codon of the downstream ORF: `-7`

The sequence of IRES region aligned to its secondary structure (if available):

aattactttt gtttattgga ataaaatgag attttttggg ttgtcatgtt aaagtgctta tagggaaaga agcctgcata taatttttta ccttgtggca 
^3622      ^3632      ^3642      ^3652      ^3662      ^3672      ^3682      ^3692      ^3702      ^3712      

taatcagtaa ttggtctgtt attcaggctt catagcttgt aaccaaatat aaataaaagg cataatttag gtattctata gttgcttaga attttgttaa 
^3722      ^3732      ^3742      ^3752      ^3762      ^3772      ^3782      ^3792      ^3802      ^3812      

tataaatctc tgtgaaaaat caaggagttt taatattttc agaagtgcat ccacctttca gggctttaag ttagtattac tcaagattat gaacaaatag 
^3822      ^3832      ^3842      ^3852      ^3862      ^3872      ^3882      ^3892      ^3902      ^3912      

cacttaggtt acctgaaaga gttactacaa ccccaaagag ttgtgttcta agtagtatct tggtaattca gagagatact catcctacct gaatataaac 
^3922      ^3932      ^3942      ^3952      ^3962      ^3972      ^3982      ^3992      ^4002      ^4012      

tgagataaat ccagtaaaga aagtgtagta aattctacat aagagtctat cattgatttc tttttgtggt aaaaatctta gttcatgtga agaaatttca 
^4022      ^4032      ^4042      ^4052      ^4062      ^4072      ^4082      ^4092      ^4102      ^4112      

tgtgaatgtt ttagctatca aacagtactg tcacctactc atgcacaaaa ctgcctccca aagacttttc ccaggtccct cgtatcaaaa cattaagagt 
^4122      ^4132      ^4142      ^4152      ^4162      ^4172      ^4182      ^4192      ^4202      ^4212      

ata
^4222

Remarks:

c-IAP1_-603/-1 IRES represents part of the human c-IAP1 5' UTR (nt from -603 to nt -1).

Citations:

The translation experiments:

Translation results:

IRESite Translation Id: 574

Version: `4`	Last change: `2008-10-23 13:18:45`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The translation method used to study IRES function:
in vivo

The organism used for translation:

Homo sapiens HEK 293T (ATCC 293tsA1609neo)

The temperature (in degrees of Celsia):
37

The relative translation efficiency in % of this IRES:

97.000

Name of IRES used as the positive control:

cIAP_-1222/-1

Name of the plasmid used as the positive control. `pbetaGAL/HIAP2(-1222/-1)/CAT`
`IRESite Id` of the plasmid used as positive control. `524`
The relative translation efficiency in % of the positive control: `100.000`
The size (length) of intercistronic region in the positive control: `1565`

The effect of 5'-cap analogs on translation:
not tested

Rapamycin affects translation:
not tested

Type of RNA subject to translation:

endogenous_nuclear_RNA_Pol_II_transcript

Remarks:

The relative IRES activity was determined in p86-cotrasfected HEK293T cells because the caspase-mediated
cleavage fragment of p97/DAP5/NAT1, p86, selectively enhances translation of c-IAP1 IRES element both in vivo
and in vitro in a manner that is independent of the translation of the upstream cistron
(Warnakulasuriyarachchi et al., 2004).

Translational data are derived from Fig. 5A.

Citations:

Last change to the database: 2019-03-18 09:32:49 GMT+1