IRESite_Id:540 characteristics of c-IAP1_-1222/-233 IRES studied in pbetaGAL/HIAP2(1-990)/CAT plasmid

IRESite: The database of experimentally verified IRES structures

The nucleic acid data:

IRESite Id: `540`	Version: `3`
Originaly submitted by: Václav Vopálenský
Reviewed by: Martin Mokrejš	Last change: `2008-10-27 23:30:37`

IRESite record type:

plasmid_with_promoter_and_putative_IRES_translationally_characterized

The shape of the nucleic acid molecule translated:
linear The quality of the mRNA/+RNA sequence:
3UTR_possibly_incomplete

The mRNA/+RNA description:

Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/HIAP2(1-990)/CAT
which comprises beta galactosidase and chloramphenicol acetyl transferase as the first and the second cistron
respectively and the part of human c-IAP1 5' UTR (nt from -1222 to nt -233 of the original sequence) mRNA
cloned between them.
The sequence ends at its 3'-end right after the poly(A) signal from BGH mRNA and thus the 3'-UTR might be
slightly wrong.

The mRNA/+RNA sequence represented in the +DNA notation:

agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc gagctcggat ccactagtaa cggccgccag 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

tgtgctggaa ttctgcagat atccatcaca ctggcggccg caattcccgg ggatcgaaag agcctgctaa agcaaaaaag aagtcaccat gtcgtttact 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

ttgaccaaca agaacgtgat tttcgttgcc ggtctgggag gcattggtct ggacaccagc aaggagctgc tcaagcgcga tcccgtcgtt ttacaacgtc 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt acagctgagc gccggtcgct 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttaaaaaa 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

cacaaacttt tggatgttcg gtttattctt tttcttttac ttttttatca tgggagccta cttcccgttt ttcccgattt ggctacatga catcaaccat 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc caaccgctgt ttggtctgct ttctgacaaa ctcggcctcg 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

actctaggcg gccgcctcga ggaattctat ggagtgtaat tttgtgtatg aattatattt ttaaaacatt gaagagtttt cagaaagaag gctagtagag 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

ttgattactg atactttatg ctaagcagta cttttttggt agtacaatat tttgttaggc gtttctgata acactagaaa ggacaagttt tatcttgtga 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

taaattgatt aatgtttaca acatgactga taattatagc tgaatagtcc ttaaatgatg aacaggttat ttagttttta aatgcagtgt aaaaagtgtg 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

ctgtggaaat tttatggcta actaagttta tggagaaaat accttcagtt gatcaagaat aatagtggta tacaaagtta ggaagaaagt caacatgatg 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

ctgcaggaaa tggaaacaaa tacaaatgat atttaacaaa gatagagttt acagtttttg aactttaagc caaattcatt tgacatcaag cactatagca 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

ggcacaggtt caacaaagct tgtgggtatt gacttccccc aaaagttgtc agctgaagta atttagccca cttaagtaaa tactatgatg ataagctgtg 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

tgaacttagc ttttaaatag tgtgaccata tgaaggtttt aattactttt gtttattgga ataaaatgag attttttggg ttgtcatgtt aaagtgctta 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

tagggaaaga agcctgcata taatttttta ccttgtggca taatcagtaa ttggtctgtt attcaggctt catagcttgt aaccaaatat aaataaaagg 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

cataatttag gtattctata gttgcttaga attttgttaa tataaatctc tgtgaaaaat caaggagttt taatattttc agaagtgcat ccacctttca 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

gggctttaag ttagtattac tcaagattat gaacaaatag cacttaggtt acctgaaaga gttactacaa ccccaaagag ttgtgttcta agtagtatct 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

tggtaattca gctcgagatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt ctgtggtgtg 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

cttatcatgt ctgcctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataa
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361

Credibility of mRNA sequence:

reverse_engineered_sequence_and_should_match_experiment_except_3UTR

The name of the plasmid:
pbetaGAL/HIAP2(1-990)/CAT The name of the promoter used to express this mRNA:
CMV

Description of the plasmid (facultative for promoter-less plasmid records):
Plasmid containing part of human c-IAP1 5' UTR (nt from -1222 to nt -233 of the original sequence) inserted between beta galactosidase and chloramphenicol acetyltransferase reporter genes.

The in vivo produced transcripts are heterogeneous (due to any of promoter?/splicing?/cleavage?/breakage?):

not tested

The in vivo produced heterogeneous transcripts occur due to alternative splicing:

not tested

A promoter reported in cDNA corresponding to IRES sequence:

not tested

The abbreviated name of the donor gene or virus from which this IRES was excised and inserted into the plasmid:
c-IAP1

The origin of IRES in the plasmid:

nuclear

The donor organism of the IRES segment:

Homo sapiens Jurkat

The DNA sequence of the plasmid in (+) orientation annotated by its secondary structure:

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc 
^1         ^11        ^21        ^31        ^41        ^51        ^61        ^71        ^81        ^91        

ccgatcccct atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
^101       ^111       ^121       ^131       ^141       ^151       ^161       ^171       ^181       ^191       

cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg 
^201       ^211       ^221       ^231       ^241       ^251       ^261       ^271       ^281       ^291       

gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat 
^301       ^311       ^321       ^331       ^341       ^351       ^361       ^371       ^381       ^391       

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 
^401       ^411       ^421       ^431       ^441       ^451       ^461       ^471       ^481       ^491       

ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 
^501       ^511       ^521       ^531       ^541       ^551       ^561       ^571       ^581       ^591       

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 
^601       ^611       ^621       ^631       ^641       ^651       ^661       ^671       ^681       ^691       

tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 
^701       ^711       ^721       ^731       ^741       ^751       ^761       ^771       ^781       ^791       

ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 
^801       ^811       ^821       ^831       ^841       ^851       ^861       ^871       ^881       ^891       

ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta ccgagctcgg atccactagt 
^901       ^911       ^921       ^931       ^941       ^951       ^961       ^971       ^981       ^991       

aacggccgcc agtgtgctgg aattctgcag atatccatca cactggcggc cgcaattccc ggggatcgaa agagcctgct aaagcaaaaa agaagtcacc 
^1001      ^1011      ^1021      ^1031      ^1041      ^1051      ^1061      ^1071      ^1081      ^1091      

atgtcgttta ctttgaccaa caagaacgtg attttcgttg ccggtctggg aggcattggt ctggacacca gcaaggagct gctcaagcgc gatcccgtcg 
^1101      ^1111      ^1121      ^1131      ^1141      ^1151      ^1161      ^1171      ^1181      ^1191      

ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 
^1201      ^1211      ^1221      ^1231      ^1241      ^1251      ^1261      ^1271      ^1281      ^1291      

caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc 
^1301      ^1311      ^1321      ^1331      ^1341      ^1351      ^1361      ^1371      ^1381      ^1391      

gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca 
^1401      ^1411      ^1421      ^1431      ^1441      ^1451      ^1461      ^1471      ^1481      ^1491      

atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt 
^1501      ^1511      ^1521      ^1531      ^1541      ^1551      ^1561      ^1571      ^1581      ^1591      

tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt 
^1601      ^1611      ^1621      ^1631      ^1641      ^1651      ^1661      ^1671      ^1681      ^1691      

ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc 
^1701      ^1711      ^1721      ^1731      ^1741      ^1751      ^1761      ^1771      ^1781      ^1791      

gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga 
^1801      ^1811      ^1821      ^1831      ^1841      ^1851      ^1861      ^1871      ^1881      ^1891      

agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt 
^1901      ^1911      ^1921      ^1931      ^1941      ^1951      ^1961      ^1971      ^1981      ^1991      

gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc 
^2001      ^2011      ^2021      ^2031      ^2041      ^2051      ^2061      ^2071      ^2081      ^2091      

gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct 
^2101      ^2111      ^2121      ^2131      ^2141      ^2151      ^2161      ^2171      ^2181      ^2191      

gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc 
^2201      ^2211      ^2221      ^2231      ^2241      ^2251      ^2261      ^2271      ^2281      ^2291      

ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg 
^2301      ^2311      ^2321      ^2331      ^2341      ^2351      ^2361      ^2371      ^2381      ^2391      

tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat 
^2401      ^2411      ^2421      ^2431      ^2441      ^2451      ^2461      ^2471      ^2481      ^2491      

ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa 
^2501      ^2511      ^2521      ^2531      ^2541      ^2551      ^2561      ^2571      ^2581      ^2591      

tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc 
^2601      ^2611      ^2621      ^2631      ^2641      ^2651      ^2661      ^2671      ^2681      ^2691      

agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg 
^2701      ^2711      ^2721      ^2731      ^2741      ^2751      ^2761      ^2771      ^2781      ^2791      

taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt 
^2801      ^2811      ^2821      ^2831      ^2841      ^2851      ^2861      ^2871      ^2881      ^2891      

aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg 
^2901      ^2911      ^2921      ^2931      ^2941      ^2951      ^2961      ^2971      ^2981      ^2991      

accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct 
^3001      ^3011      ^3021      ^3031      ^3041      ^3051      ^3061      ^3071      ^3081      ^3091      

gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt 
^3101      ^3111      ^3121      ^3131      ^3141      ^3151      ^3161      ^3171      ^3181      ^3191      

aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag 
^3201      ^3211      ^3221      ^3231      ^3241      ^3251      ^3261      ^3271      ^3281      ^3291      

aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag 
^3301      ^3311      ^3321      ^3331      ^3341      ^3351      ^3361      ^3371      ^3381      ^3391      

cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg 
^3401      ^3411      ^3421      ^3431      ^3441      ^3451      ^3461      ^3471      ^3481      ^3491      

ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct 
^3501      ^3511      ^3521      ^3531      ^3541      ^3551      ^3561      ^3571      ^3581      ^3591      

ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca 
^3601      ^3611      ^3621      ^3631      ^3641      ^3651      ^3661      ^3671      ^3681      ^3691      

gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca 
^3701      ^3711      ^3721      ^3731      ^3741      ^3751      ^3761      ^3771      ^3781      ^3791      

ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc 
^3801      ^3811      ^3821      ^3831      ^3841      ^3851      ^3861      ^3871      ^3881      ^3891      

ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga 
^3901      ^3911      ^3921      ^3931      ^3941      ^3951      ^3961      ^3971      ^3981      ^3991      

attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg 
^4001      ^4011      ^4021      ^4031      ^4041      ^4051      ^4061      ^4071      ^4081      ^4091      

cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga 
^4101      ^4111      ^4121      ^4131      ^4141      ^4151      ^4161      ^4171      ^4181      ^4191      

gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta 
^4201      ^4211      ^4221      ^4231      ^4241      ^4251      ^4261      ^4271      ^4281      ^4291      

ctatttaaaa aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt ttttcccgat ttggctacat 
^4301      ^4311      ^4321      ^4331      ^4341      ^4351      ^4361      ^4371      ^4381      ^4391      

gacatcaacc atatcagcaa aagtgatacg ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca 
^4401      ^4411      ^4421      ^4431      ^4441      ^4451      ^4461      ^4471      ^4481      ^4491      

aactcggcct cgactctagg cggccgcctc gaggaattct atggagtgta attttgtgta tgaattatat ttttaaaaca ttgaagagtt ttcagaaaga 
^4501      ^4511      ^4521      ^4531      ^4541      ^4551      ^4561      ^4571      ^4581      ^4591      

aggctagtag agttgattac tgatacttta tgctaagcag tacttttttg gtagtacaat attttgttag gcgtttctga taacactaga aaggacaagt 
^4601      ^4611      ^4621      ^4631      ^4641      ^4651      ^4661      ^4671      ^4681      ^4691      

tttatcttgt gataaattga ttaatgttta caacatgact gataattata gctgaatagt ccttaaatga tgaacaggtt atttagtttt taaatgcagt 
^4701      ^4711      ^4721      ^4731      ^4741      ^4751      ^4761      ^4771      ^4781      ^4791      

gtaaaaagtg tgctgtggaa attttatggc taactaagtt tatggagaaa ataccttcag ttgatcaaga ataatagtgg tatacaaagt taggaagaaa 
^4801      ^4811      ^4821      ^4831      ^4841      ^4851      ^4861      ^4871      ^4881      ^4891      

gtcaacatga tgctgcagga aatggaaaca aatacaaatg atatttaaca aagatagagt ttacagtttt tgaactttaa gccaaattca tttgacatca 
^4901      ^4911      ^4921      ^4931      ^4941      ^4951      ^4961      ^4971      ^4981      ^4991      

agcactatag caggcacagg ttcaacaaag cttgtgggta ttgacttccc ccaaaagttg tcagctgaag taatttagcc cacttaagta aatactatga 
^5001      ^5011      ^5021      ^5031      ^5041      ^5051      ^5061      ^5071      ^5081      ^5091      

tgataagctg tgtgaactta gcttttaaat agtgtgacca tatgaaggtt ttaattactt ttgtttattg gaataaaatg agattttttg ggttgtcatg 
^5101      ^5111      ^5121      ^5131      ^5141      ^5151      ^5161      ^5171      ^5181      ^5191      

ttaaagtgct tatagggaaa gaagcctgca tataattttt taccttgtgg cataatcagt aattggtctg ttattcaggc ttcatagctt gtaaccaaat 
^5201      ^5211      ^5221      ^5231      ^5241      ^5251      ^5261      ^5271      ^5281      ^5291      

ataaataaaa ggcataattt aggtattcta tagttgctta gaattttgtt aatataaatc tctgtgaaaa atcaaggagt tttaatattt tcagaagtgc 
^5301      ^5311      ^5321      ^5331      ^5341      ^5351      ^5361      ^5371      ^5381      ^5391      

atccaccttt cagggcttta agttagtatt actcaagatt atgaacaaat agcacttagg ttacctgaaa gagttactac aaccccaaag agttgtgttc 
^5401      ^5411      ^5421      ^5431      ^5441      ^5451      ^5461      ^5471      ^5481      ^5491      

taagtagtat cttggtaatt cagctcgaga tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 
^5501      ^5511      ^5521      ^5531      ^5541      ^5551      ^5561      ^5571      ^5581      ^5591      

atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg 
^5601      ^5611      ^5621      ^5631      ^5641      ^5651      ^5661      ^5671      ^5681      ^5691      

gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt 
^5701      ^5711      ^5721      ^5731      ^5741      ^5751      ^5761      ^5771      ^5781      ^5791      

gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 
^5801      ^5811      ^5821      ^5831      ^5841      ^5851      ^5861      ^5871      ^5881      ^5891      

ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat 
^5901      ^5911      ^5921      ^5931      ^5941      ^5951      ^5961      ^5971      ^5981      ^5991      

ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg 
^6001      ^6011      ^6021      ^6031      ^6041      ^6051      ^6061      ^6071      ^6081      ^6091      

ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg 
^6101      ^6111      ^6121      ^6131      ^6141      ^6151      ^6161      ^6171      ^6181      ^6191      

cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac 
^6201      ^6211      ^6221      ^6231      ^6241      ^6251      ^6261      ^6271      ^6281      ^6291      

ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc 
^6301      ^6311      ^6321      ^6331      ^6341      ^6351      ^6361      ^6371      ^6381      ^6391      

taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat 
^6401      ^6411      ^6421      ^6431      ^6441      ^6451      ^6461      ^6471      ^6481      ^6491      

gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg 
^6501      ^6511      ^6521      ^6531      ^6541      ^6551      ^6561      ^6571      ^6581      ^6591      

ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta 
^6601      ^6611      ^6621      ^6631      ^6641      ^6651      ^6661      ^6671      ^6681      ^6691      

tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa 
^6701      ^6711      ^6721      ^6731      ^6741      ^6751      ^6761      ^6771      ^6781      ^6791      

taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag 
^6801      ^6811      ^6821      ^6831      ^6841      ^6851      ^6861      ^6871      ^6881      ^6891      

ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 
^6901      ^6911      ^6921      ^6931      ^6941      ^6951      ^6961      ^6971      ^6981      ^6991      

tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
^7001      ^7011      ^7021      ^7031      ^7041      ^7051      ^7061      ^7071      ^7081      ^7091      

tcatcaatgt atcttatcat gtctgcctag agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg 
^7101      ^7111      ^7121      ^7131      ^7141      ^7151      ^7161      ^7171      ^7181      ^7191      

ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 
^7201      ^7211      ^7221      ^7231      ^7241      ^7251      ^7261      ^7271      ^7281      ^7291      

tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 
^7301      ^7311      ^7321      ^7331      ^7341      ^7351      ^7361      ^7371      ^7381      ^7391      

gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
^7401      ^7411      ^7421      ^7431      ^7441      ^7451      ^7461      ^7471      ^7481      ^7491      

gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 
^7501      ^7511      ^7521      ^7531      ^7541      ^7551      ^7561      ^7571      ^7581      ^7591      

ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
^7601      ^7611      ^7621      ^7631      ^7641      ^7651      ^7661      ^7671      ^7681      ^7691      

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 
^7701      ^7711      ^7721      ^7731      ^7741      ^7751      ^7761      ^7771      ^7781      ^7791      

ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 
^7801      ^7811      ^7821      ^7831      ^7841      ^7851      ^7861      ^7871      ^7881      ^7891      

gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 
^7901      ^7911      ^7921      ^7931      ^7941      ^7951      ^7961      ^7971      ^7981      ^7991      

gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
^8001      ^8011      ^8021      ^8031      ^8041      ^8051      ^8061      ^8071      ^8081      ^8091      

ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 
^8101      ^8111      ^8121      ^8131      ^8141      ^8151      ^8161      ^8171      ^8181      ^8191      

ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 
^8201      ^8211      ^8221      ^8231      ^8241      ^8251      ^8261      ^8271      ^8281      ^8291      

aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 
^8301      ^8311      ^8321      ^8331      ^8341      ^8351      ^8361      ^8371      ^8381      ^8391      

gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg 
^8401      ^8411      ^8421      ^8431      ^8441      ^8451      ^8461      ^8471      ^8481      ^8491      

ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 
^8501      ^8511      ^8521      ^8531      ^8541      ^8551      ^8561      ^8571      ^8581      ^8591      

tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 
^8601      ^8611      ^8621      ^8631      ^8641      ^8651      ^8661      ^8671      ^8681      ^8691      

agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 
^8701      ^8711      ^8721      ^8731      ^8741      ^8751      ^8761      ^8771      ^8781      ^8791      

ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 
^8801      ^8811      ^8821      ^8831      ^8841      ^8851      ^8861      ^8871      ^8881      ^8891      

tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 
^8901      ^8911      ^8921      ^8931      ^8941      ^8951      ^8961      ^8971      ^8981      ^8991      

ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 
^9001      ^9011      ^9021      ^9031      ^9041      ^9051      ^9061      ^9071      ^9081      ^9091      

ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 
^9101      ^9111      ^9121      ^9131      ^9141      ^9151      ^9161      ^9171      ^9181      ^9191      

ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 
^9201      ^9211      ^9221      ^9231      ^9241      ^9251      ^9261      ^9271      ^9281      ^9291      

aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt 
^9301      ^9311      ^9321      ^9331      ^9341      ^9351      ^9361      ^9371      ^9381      ^9391      

cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 
^9401      ^9411      ^9421      ^9431      ^9441      ^9451      ^9461      ^9471      ^9481      ^9491      

aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
^9501      ^9511      ^9521      ^9531      ^9541      ^9551      ^9561      ^9571      ^9581      ^9591      

cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
^9601      ^9611      ^9621      ^9631      ^9641      ^9651      ^9661      ^9671      ^9681      ^9691      

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
^9701      ^9711      ^9721      ^9731      ^9741      ^9751      ^9761      ^9771      ^9781      ^9791      

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
^9801      ^9811      ^9821      ^9831      ^9841      ^9851      ^9861      ^9871      ^9881      ^9891      

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
^9901      ^9911      ^9921      ^9931      ^9941      ^9951      ^9961      ^9971      ^9981      ^9991      

tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
^10001     ^10011     ^10021     ^10031     ^10041     ^10051     ^10061     ^10071     ^10081     ^10091     

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
^10101     ^10111     ^10121     ^10131     ^10141     ^10151     ^10161     ^10171     ^10181     ^10191     

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 
^10201     ^10211     ^10221     ^10231     ^10241     ^10251     ^10261     ^10271     ^10281     ^10291     

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
^10301     ^10311     ^10321     ^10331     ^10341     ^10351     ^10361     ^10371     ^10381     ^10391     

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
^10401     ^10411     ^10421     ^10431     ^10441     ^10451     ^10461     ^10471     ^10481     ^10491     

ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
^10501     ^10511     ^10521     ^10531     ^10541     ^10551     ^10561     ^10571     ^10581     ^10591     

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
^10601     ^10611     ^10621     ^10631     ^10641     ^10651     ^10661     ^10671     ^10681     ^10691     

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
^10701     ^10711     ^10721     ^10731     ^10741     ^10751     ^10761     ^10771     ^10781     ^10791     

agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
^10801     ^10811     ^10821     ^10831     ^10841     ^10851     ^10861     ^10871     ^10881     ^10891     

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
^10901     ^10911     ^10921     ^10931     ^10941     ^10951     ^10961     ^10971     ^10981     ^10991     

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
^11001     ^11011     ^11021     ^11031     ^11041     ^11051     ^11061     ^11071     ^11081     ^11091     

cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
^11101     ^11111     ^11121     ^11131     ^11141     ^11151     ^11161     ^11171     ^11181     ^11191     

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
^11201     ^11211     ^11221     ^11231     ^11241     ^11251     ^11261     ^11271     ^11281     ^11291     

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
^11301     ^11311     ^11321     ^11331     ^11341     ^11351     ^11361     ^11371     ^11381     ^11391     

caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
^11401     ^11411     ^11421     ^11431     ^11441     ^11451     ^11461     ^11471     ^11481     ^11491     

tcatgag
^11501

GenBank formatted file with annotated plasmid sequence hyperlinked from vector image map:

The total number of notable open-reading frames (ORFs):

2

Notable Open-Reading Frames (ORFs; protein coding regions) in the mRNA/+RNA sequence:

ORF

ORF position:

1

Version: `0`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
LacZ

The description of the protein encoded in this ORF:
beta galactosidase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

189-3332

ORF

ORF position:

2

Version: `1`	Last change: `2008-10-27 23:30:37`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The abbreviated name of this ORF/gene:
CAT

The description of the protein encoded in this ORF:
chloramphenicol acetyltransferase

The translational frameshift (ribosome slippage) involved:
0 The ribosome read-through involved:
no

The alternative forms of this protein occur by the alternative initiation of translation:

not tested

The ORF absolute position (the base range includes START and STOP codons or their equivalents):

4618-5277

Citations:

Warnakulasuriyarachchi D., Cerquozzi S., Cheung H. H., Holcik M. (2004) Translational induction of the inhibitor of apoptosis protein HIAP2 during endoplasmic reticulum stress attenuates cell death and is mediated via an inducible internal ribosome entry site element. J. Biol. Chem. 279(17):17148-17157

IRESs:

IRES:

Version: `1`	Last change: `2008-10-23 13:11:27`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš
The IRES name: `c-IAP1_-1222/-233`	The functional status of IRES: `defective`

The IRES absolute position (the range includes START and STOP codons or their equivalents):

3622-4611

How IRES boundaries were determined:
experimentally_determined

5'-end of IRES relative to last base of the STOP codon of the upstream ORF: `290`	3'-end of IRES relative to last base of the STOP codon of the upstream ORF: `1279`
5'-end of IRES relative to first base of the START codon of the downstream ORF: `-996`	3'-end of IRES relative to first base of the START codon of the downstream ORF: `-7`

The sequence of IRES region aligned to its secondary structure (if available):

gaattctatg gagtgtaatt ttgtgtatga attatatttt taaaacattg aagagttttc agaaagaagg ctagtagagt tgattactga tactttatgc 
^3622      ^3632      ^3642      ^3652      ^3662      ^3672      ^3682      ^3692      ^3702      ^3712      

taagcagtac ttttttggta gtacaatatt ttgttaggcg tttctgataa cactagaaag gacaagtttt atcttgtgat aaattgatta atgtttacaa 
^3722      ^3732      ^3742      ^3752      ^3762      ^3772      ^3782      ^3792      ^3802      ^3812      

catgactgat aattatagct gaatagtcct taaatgatga acaggttatt tagtttttaa atgcagtgta aaaagtgtgc tgtggaaatt ttatggctaa 
^3822      ^3832      ^3842      ^3852      ^3862      ^3872      ^3882      ^3892      ^3902      ^3912      

ctaagtttat ggagaaaata ccttcagttg atcaagaata atagtggtat acaaagttag gaagaaagtc aacatgatgc tgcaggaaat ggaaacaaat 
^3922      ^3932      ^3942      ^3952      ^3962      ^3972      ^3982      ^3992      ^4002      ^4012      

acaaatgata tttaacaaag atagagttta cagtttttga actttaagcc aaattcattt gacatcaagc actatagcag gcacaggttc aacaaagctt 
^4022      ^4032      ^4042      ^4052      ^4062      ^4072      ^4082      ^4092      ^4102      ^4112      

gtgggtattg acttccccca aaagttgtca gctgaagtaa tttagcccac ttaagtaaat actatgatga taagctgtgt gaacttagct tttaaatagt 
^4122      ^4132      ^4142      ^4152      ^4162      ^4172      ^4182      ^4192      ^4202      ^4212      

gtgaccatat gaaggtttta attacttttg tttattggaa taaaatgaga ttttttgggt tgtcatgtta aagtgcttat agggaaagaa gcctgcatat 
^4222      ^4232      ^4242      ^4252      ^4262      ^4272      ^4282      ^4292      ^4302      ^4312      

aattttttac cttgtggcat aatcagtaat tggtctgtta ttcaggcttc atagcttgta accaaatata aataaaaggc ataatttagg tattctatag 
^4322      ^4332      ^4342      ^4352      ^4362      ^4372      ^4382      ^4392      ^4402      ^4412      

ttgcttagaa ttttgttaat ataaatctct gtgaaaaatc aaggagtttt aatattttca gaagtgcatc cacctttcag ggctttaagt tagtattact 
^4422      ^4432      ^4442      ^4452      ^4462      ^4472      ^4482      ^4492      ^4502      ^4512      

caagattatg aacaaatagc acttaggtta cctgaaagag ttactacaac cccaaagagt tgtgttctaa gtagtatctt ggtaattcag
^4522      ^4532      ^4542      ^4552      ^4562      ^4572      ^4582      ^4592      ^4602

Remarks:

c-IAP1_-1222/-233 IRES represents part of the human c-IAP1 5' UTR (nt from -1222 to nt -233).

Citations:

The translation experiments:

Translation results:

IRESite Translation Id: 588

Version: `2`	Last change: `2008-10-22 13:31:58`
Originaly submitted by: Václav Vopálenský	Reviewed by: Martin Mokrejš

The translation method used to study IRES function:
in vivo

The organism used for translation:

Homo sapiens HEK 293T (ATCC 293tsA1609neo)

The temperature (in degrees of Celsia):
37

The relative translation efficiency in % of this IRES:

2.000

Name of IRES used as the positive control:

cIAP1_-150/-1

Name of the plasmid used as the positive control. `pbetaGAL/HIAP2(1073-1222)/CAT`
`IRESite Id` of the plasmid used as positive control. `530`
The relative translation efficiency in % of the positive control: `100.000`
The size (length) of intercistronic region in the positive control: `445`

The effect of 5'-cap analogs on translation:
not tested

Rapamycin affects translation:
not tested

Type of RNA subject to translation:

endogenous_nuclear_RNA_Pol_II_transcript

Remarks:

Translational efficiency was determined in thapsigargin (resuspended in DMSO)-treated cells.

Translational data are derived from Fig. 5C.

Citations:

Last change to the database: 2019-03-18 09:32:49 GMT+1